Natural and synthetic compounds for treating cancer and other diseases

ABSTRACT

This invention provides a method of synthesizing new active compounds for pharmaceutical uses including cancer treatment, wherein the cancers comprise breast, leukocytic, liver, ovarian, bladder, prostatic, skin, bone, brain, leukemia, lung, colon, CNS, melanoma, renal, cervical, esophageal, testicular, spleenic, kidney, lymphatic, pancreatic, stomach and thyroid cancers. This invention is an anti-adhesion therapy which uses the compound as a mediator or inhibitor of adhesion proteins and angiopoietins. It inhibits excess adhesion and inhibits cell attachment. It modulates angiogenesis. The compounds also use as mediator of cell adhesion receptor, cell circulating, cell moving and inflammatory diseases. The compounds are attached with angeloyl, acetyl, tigloyl, senecioyl, Crotonoyl, 3,3-Dimethylartyloyl, Cinnamoyl, Pentenoyl, Hexanoyl, benzoyl, Ethylbutyryl, benzoyl, dibenzoyl, alkanoyl, alkenoyl, benzoyl alkyl substituted alkanoyl, ethanoyl, propanoyl, propenoyl, butanoyl, butenoyl, pentanoyl, hexenoyl, heptanoyl, heptenoyl, octanoyl, octenoyl, nonanoyl, nonenoyl, decanoyl, decenoyl, propionyl, 2-propenoyl, 2-butenoyl, Isobutyryl, 2-methylpropanoyl, 2-ethylbutyryl, ethylbutanoyl, 2-ethylbutanoyl, butyryl, (E)-2,3-Dimethylacryloyl, (E)-2-Methylcrotonoyl, 3-cis-Methyl-methacryloyl, 3-Methyl-2-butenoyl, 3-Methylcrotonoyl, 4-Pentenoyl, (2E)-2-pentenoyl, Caproyl, 5-Hexenoyl, Capryloyl, Lauroyl, Dodecanoyl, Myristoyl, Tetradecanoyl, Oleoyl, O—C(2-18) Acyl.

FIELD OF THE INVENTION

This invention provides methods of synthesing new compounds forpharmaceutical uses.

BACKGROUND OF THE INVENTION

This invention provides methods of synthesing new compounds forpharmaceutical uses. This invention provides methods, compounds andcompositions for treating cancer, inhibiting cancer invasion, cellinvasion, or cancer cell invasion, wherein the cancers comprise breast,leukocytic, liver, ovarian, bladder, prostatic, skin, bone, brain,leukemia, lung, colon, CNS, melanoma, renal, cervical, esophageal,testicular, spleenic, kidney, lymphatic, pancreatic, stomach and thyroidcancers.

SUMMARY OF THE INVENTION

This invention provides methods of synthesizing new compounds forpharmaceutical uses. This invention provides compounds, compositions,and methods for treating cancer, inhibiting cancer invasion, cellinvasion, macromolecular invasion, cancer cell invasion, and metastasis.This invention provides a use of compounds, compositions, formanufacturing medicament for treating cancer, inhibiting cancerinvasion, macromolecular invasion, virus invasion and metastasis. Thisinvention provides compounds for use as mediator or inhibitor ofadhesion protein or angiopoietin, This invention provides compounds foruse in a method of modulating attachment or adhesion of cells orangiogenesis, by modulating or inhibiting adhesion proteinmacromolecules, or angiopoietin, The compounds comprise the structuresselected from the formulae in the present application, wherein thecompounds are synthesized or isolated, wherein the compounds comprisethe saponins, triterpenes, pentacyclic triterpenes, and compoundsselected from formulae in the present application, wherein the cancerscomprise breast, leukocytic, liver, ovarian, bladder, prostatic, skin,bone, brain, leukemia, lung, colon, CNS, melanoma, renal, cervical,esophageal, testicular, spleenic, kidney, lymphatic, pancreatic, stomachand thyroid cancers. This invention provides compounds for use as amediator for cell circulating, cell moving cell homing and inflammatorydiseases. This invention provides compounds for improving bloodcirculation; soothing stroke; preventing plaque formation and promotetheir dissipated; improve blood viscosity; reducing cardiovascular;reducing cerebrovascular; reducing thrombosis, arteriosclerosis,coronary heart disease, hypertension, diabetes, thrombocytopeniapurpura, hemoptysis, hematemesis; treating blood in the stool, uterinebleeding, traumatic bleeding, abdominal irritation, swelling,fluttering, Blood circulation, swelling, pain; Treating bronchiectasis,tuberculosis and lung abscess caused by too hemoptysis; reducingbleeding, antitussive, expectorant and analgesic effect, dilate bloodvessels; reducing blood pressure and the treatment of cerebralarteriosclerosis; elevating blood lipids and reducing cholesterol.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1. HPLC profiles of esterification products of E4A with Tigloylchloride (A) from different times of esterification reaction. Reactionproducts obtained from each time of reaction (5 sec, 1 min, 2 min, 5min, and 10 min) were fractionated by HPLC. The profile is plottedaccording to HPLC elution time and optical density of fractions.Reaction was performed at Room temperature (Top row) and 0 C (bottomrow).

FIG. 2. HPLC profiles of esterification products of E4A with3,3-dimethylacryloly chloride (B) from different times of esterificationreaction. Reaction products obtained from each time of reaction (5 sec,1 min, 2 min, 5 min, and 10 min) were fractionated by HPLC. The profileis plotted according to HPLC elution time and optical density offractions. Reaction was performed at Room temperature (Top row) and 0 C(bottom row).

FIG. 3. MTT cytotoxic activity of times study at room temperature, A:E4A-Tigloyl(A); B: E4A-3,3-dimethylacryloly(B); C: E4A-4-pentenoyl(C).

FIG. 4. MTT cytotoxic activity of times study at 0 C, A: E4A-Tigloyl(A);B: E4A-3,3-dimethylacryloly(B); C: E4A-4-pentenoyl(C).

FIG. 5. MTT cytotoxic activity of times study, A: E4A-cinnamoyl(J); B:E4A-hexanoyl(D); C: E4A-2-ethylbutyryl(E); and D, controls: Tig controlis tigloyl chloride without E4A; AC control is acetyl chloride withoutE4A; H is acetyl chloride with E4A reaction 1 min.

FIG. 6. MTT cytotoxic activity of times study, A: E4A-acetyl(H); B:E4A-crotonoyl(I)

FIG. 7. HPLC profiles of E4A-Tig in 1 min and 2 hours

FIG. 8. MTT cytotoxic activity of times study for E4A-Tig. Results:E4A-Tigs from reaction of 5 sec to 1 min are most active. Activitydecrease after 1 min of reaction. Minimum to no activity was obtained at10 minutes or longer.

FIG. 9. Results of HPLC profiles of E4A-Tigs: E4A, E4A-ASAP (5 sec),E4A-1 min, E4A-2 min, E4A-5 min, E4A-10 min, E4A-30 min.

FIG. 10. Results of Activity order: M, N, O, P, Q, R, S, T, E4A; M=E4Ahas no activity.

FIG. 11. (A) The IC50 of Tig-S in KB cells is about 4 ug/ml; and thecorresponding IC50 in ES2 cells is less than 1 ug/ml; (B) The IC50 ofTig-S in ES2 cells, MTT assay with low doses of Tig-S, the IC50 of Tig-Sin ES2 cells is approximately equal to 0.1 ug/ml

FIG. 12. (A) Results: Swiss3T3 cells are mouse normal fibroblast whichwere used in this experiment to compare with ES2 (human ovarian cancer)in Tig-R cytotoxicity determination. The preliminary results indicatethat the IC50 of Tig-R in SW3T3 cells is above 20 ug/ml while thecorresponding IC50 in ES2 cells is about 2.8 ug/ml. (B) Effect of Tig-Ron Normal human lung fibroblast (W138). Results: The IC50 of Tig-R innormal human fibroblast cells (W138) is about 10-15 ug/ml. This IC50value is 3 times higher than those in ES2 (3 ug/ml).

FIG. 13. (A) Results: Tig-N, -Q, -R, -T -S and -V do not have hemolyticactivity up to 20 ug/ml. The graphs results are overlapped at the bottomof Figure. The original compound ES lyse 100% red blood cells (RBC) at 5ug/ml. (B) Results: compare to Y3, the ACH-Y3 is less potent inhemolytic activity. Tig-R has no hemolytic activity

FIG. 14. (A) Comparison of potency of compound Y in inhibiting growth ofovarian cancer cells. The IC50 for Compound Y is about 1.51 μg/ml. (B).Hemolytic activity of Xanifolia-Y, B-Escin, Xanifolia-X, ACH-Y andAKOH-Y

FIG. 15. Inhibition of W138 cells with Tig-S (6 days); Result: IC50=1.5ug/ml; At 10-20 ug/ml, about negative 10% cell growth.

FIG. 16. Inhibition of ES2 cells with Tig-S (2 days); Results: IC50=0.3ug/ml; At 5-10 ug/ml, negative 70% cells growth.

FIG. 17. A comparison of non-cancerous W138 with ES2 cancer cells. MTTOD is proportional to the amount of live cells. Here, the MTT OD in theno drug control represents 100% of cell growth. This study shows that at10 ug/ml of Tig-S, W138 cells maintain about 55% of the control cellgrowth, while ES2 cells have only 10% of the control cell growth.

FIG. 18. A comparison of non-cancerous W138 with cancer cells: In theseresults, the MTT OD from cells before and after treated with differentconcentrations of drug was plotted. The result shows that: For W138cells, the IC₁₀₀ value is about 10 ug/ml [IC₁₀₀ is defined as the MTT ODvalue after the drug-treatment equal to the original OD value before thedrug-treatment. At this condition (IC₁₀₀), it indicates there is 100%inhibition of growth, but there is no cell lost]. At 20 ug/ml, the ODdecrease to about 90% of the original value, indicating there is about10% cell lost or dead. For ES2 cells, the IC₁₀₀ value is about 0.16-0.3ug/ml. However, there is a big decrease of OD with higher drugconcentrations indicating there are cell dead. At 10 ug/ml, the OD is12% of the original value, indicating over 90% cells cell lost.

FIG. 19. Tig-S induces cell-death by the apoptosis mechanism.

FIG. 20-21. Leukemia K562 cells were treated with Tig-S for three days;The number inside charts is the Tig-S concentration in ug/ml; The firstpeak is the intensity of G0/G1 cells. The last peak is the G2/M cells.Between these two peaks represents the intensity of S-phase cells.

FIG. 22. Animal study result shows Group A Mice—Implanted tumor and nodrug, Died on day 27; Group B Mice—Implanted tumor and with (Tig-S) drug100 mg/kg, twice daily, 5 days.

FIG. 23. Animal study result shows Group A Mice—Implanted tumor and nodrug; Group B Mice—Implanted tumor and with (Tig-R) drug 100 mg/kg,twice daily, 5 days.

FIG. 24. Animal study result shows Group A Mice—Implanted tumor and nodrug; Group B Mice—Implanted tumor and with (Tig-V) drug 50 mg/kg, twicedaily, 10 days.

FIG. 25. Inhibition of lung H460 cells growth with Tig-S for one day.IC50=3.4 ug/ml

FIG. 26. Inhibition of lung H460 cells growth with Tig-S for 4 days.IC50=3 ug/ml

FIG. 27. Inhibition of Leukemia K562 cells by Tig-S: Tig-S inhibitsLeukemia K562 cells growth with IC50 about 0.6 ug/ml. No grow (IC100)was observed beginning on day 2 at 2.5 ug/ml or higher.

FIG. 28. Inhibition of cancer OVCAR3 cells by natural compounds escin,X, Y0, Y1, Y2, Y3, Y7, Y8, Y9, Y10

FIG. 29. Compare the activities of natural compound Y and syntheticcompound Tig-S and Tig-R with leukemia cancer. The synthetic compoundsincrease the potency and decrease the toxicity. A: Natural compound; B:synthetic compound Tig-S; C: synthetic compound Tig-R.

FIG. 30. Compare the activities of natural compound Y and syntheticcompound Tig-S and Tig-R with prostate cancer. The synthetic compoundsincrease the potency and decrease the toxicity. A: Natural compound; B:synthetic compound Tig-S; C: synthetic compound Tig-R.

FIG. 31-33. Drug-effects of Tig-R

FIG. 34-36. Drug-effects of Tig-S

FIG. 37. Inhibition of overian cancer (OCAR3) by Tig-s, Tig-S inhibitsOCAR3 cells' growth with an IC₅₀ value of 2.5 ug/ml; and inhibition ofpancreas cancer (Capan), Tig-S inhibits Capan cells' growth with an IC₅₀value of about 1 ug/ml.

DETAILED DESCRIPTION OF THE INVENTION

This invention provides a method of synthesising new active compoundsfor pharmaceutical uses. This invention provides an anti adhesiontherapy which uses the compound as a mediator or inhibitor of adhesionproteins and angiopoietins. It inhibits excessive adhesion and inhibitscell viral and macromolecular attachment. It modulates angiogenesis. Thecompounds also use as mediator of cell viral and macromolecular adhesionreceptor(s).

This invention provides compounds or a composition comprising thecompounds provided in the invention for treating cancers; for inhibitingcancer growth, for inhibiting viruses; for preventing cerebral aging;for improving memory; improving cerebral functions; for curing enuresis,frequent micturition, urinary incontinence; neurodegenerative diseases,dementia, Alzheimer's disease, autism, brain trauma, Parkinson's diseaseor other diseases caused by cerebral dysfunctions; for treatingarthritis, rheumatism, poor circulation, arteriosclerosis, Raynaud'ssyndrome, angina pectoris, cardiac disorder, coronary heart disease,headache, dizziness, kidney disorder; cerebrovascular diseasea;inhibiting NF-kappa B activation; for treating brain edema, severe acuterespiratory syndrome, respiratory viral diseases, chronic venousinsufficiency, hypertension, chronic venous disease, oedema,inflammation, hemonhoids, peripheral edema formation, varicose veindisease, flu, post traumatic edema and postoperative swelling; forinhibiting blood clots, for inhibiting ethanol absorption; for loweringblood sugar; for regulating adrenocorticotropin and corticosteronelevels. This invention provides a composition for Anti-MS,anti-aneurysm, anti-asthmatic, anti-oedematous, anti-inflammatory,anti-bradykinic, anti-capillarihemorrhagic, anti-cephalagic,anti-cervicobrachialgic, anti-eclamptic, anti-edemic, anti-encaphalitic,anti-epiglottitic, anti-exudative, anti-flu, anti-fracture,anti-gingivitic, anti-hematomic, anti-herpetic, anti-histaminic,anti-hydrathritic, anti-meningitic, antioxidant, anti-periodontic,anti-phlebitic, anti-pleuritic, anti-raucedo, anti-rhinitic,anti-tonsilitic, anti-ulcer, anti-varicose, anti-vertiginous,cancerostatic, corticosterogenic, diuretic, fungicide, hemolytic,hyaluronidase inhibitor, lymphagogue, natriuretic, pesticide, pituitarystimulant, thymolytic, vasoprotective, inhibiting leishmaniases,modulating adhesion or angiogenesis of cells, anti-parasitic; increasethe expression of the genes: ANGPT2, DDIT3, LIF and NFKB1Z, andmanufacturing an adjuvant composition and venotonic treatment.

This invention provides compounds, compositions and methods for treatingcancer diseases, inhibiting cancer invasion, for inhibiting cancergrowth or for inhibiting cancer metastasis, wherein the compoundscomprise the structures selected from the formulae of the presentapplication, wherein the compounds can be synthesized or isolated,wherein the compounds comprise the triterpenes, pentacyclic triterpenes,saponins, and compounds selected from formulae in this application,wherein the cancers comprise breast cancer, leukocytic cancer, livercancer, ovarian cancer, bladder cancer, prostatic cancer, skin cancer,bone cancer, brain cancer, leukemia cancer, lung cancer, colon cancer,CNS cancer, melanoma cancer, renal cancer, cervical cancer, esophagealcancer, testicular cancer, spleenic cancer, kidney cancer, lymphhaticcancer, pancreatic cancer, stomach cancer and thyroid cancer; whereinthe cells comprise breast cell, leukocytic cell, liver cell, ovariancell, bladder cell, prostatic cell, skin cell, bone cell, brain cell,leukemia cell, lung cell, colon cell, CNS cell, melanoma cell, renalcell, cervical cell, esophageal cell, testicular cell, spleenic cell,kidney cell, lymphhatic cell, pancreatic cell, stomach cell and thyroidcell.

This invention provides compounds for improving blood circulation;soothing stroke; preventing plaque formation and promote theirdissipated; improve blood viscosity; reducing cardiovascular; reducingcerebrovascular; reducing thrombosis, arteriosclerosis, coronary heartdisease, hypertension, diabetes, thrombocytopenia purpura, hemoptysis,hematemesis; treating blood in the stool, uterine bleeding, traumaticbleeding, abdominal irritation, swelling, fluttering, Blood circulation,swelling, pain; Treating bronchiectasis, tuberculosis and lung abscesscaused by too hemoptysis; reducing bleeding, antitussive, expectorantand analgesic effect, dilate blood vessels; reducing blood pressure andthe treatment of cerebral arteriosclerosis; elevating blood lipids andreducing cholesterol.

This invention shows that the presence of group selected from acetyl,angeloyl, tigloyl, senecioyl, Crotonoyl, 3,3-Dimethylartyloyl,Cinnamoyl, Pentenoyl, Hexanoyl, benzoyl, Ethylbutyryl, alkyl, dibenzoyl,benzoyl, methylbutanoyl, methylpropanoyl, alkanoyl, alkenoyl, benzoylalkyl substituted alkanoyl, alkanoyl substituted phenyl, alkenoylsubstituted phenyl, aryl, acyl, heterocylic, heteroraryl,alkenylcarbonyl, ethanoyl, propanoyl, propenoyl, butanoyl, butenoyl,pentanoyl, hexenoyl, heptanoyl, heptenoyl, octanoyl, octenoyl, nonanoyl,nonenoyl, decanoyl, decenoyl, propionyl, 2-propenoyl, 2-butenoyl,Isobutyryl, 2-methylpropanoyl, 2-ethylbutyryl, ethylbutanoyl,2-ethylbutanoyl, butyryl, (E)-2,3-Dimethylacryloyl,(E)-2-Methylcrotonoyl, 3-cis-Methyl-methacryloyl, 3-Methyl-2-butenoyl,3-Methylcrotonoyl, 4-Pentenoyl, (2E)-2-pentenoyl, Caproyl, 5-Hexenoyl,Capryloyl, Lauroyl, Dodecanoyl, Myristoyl, Tetradecanoyl, Oleoyl,C(2-18) Acyl, or sugar moiety substituted with acetyl, angeloyl,tigloyl, senecioyl, Crotonoyl, 3,3-Dimethylartyloyl, Cinnamoyl,Pentenoyl, Hexanoyl, benzoyl, Ethylbutyryl, alkyl, dibenzoyl, benzoyl,methylbutanoyl, methylpropanoyl, alkanoyl, alkenoyl, benzoyl alkylsubstituted alkanoyl, alkanoyl substituted phenyl, alkenoyl substitutedphenyl, aryl, acyl, heterocylic, heteroraryl, alkenylcarbonyl, ethanoyl,propanoyl, propenoyl, butanoyl, butenoyl, pentanoyl, hexenoyl,heptanoyl, heptenoyl, octanoyl, octenoyl, nonanoyl, nonenoyl, decanoyl,decenoyl, propionyl, 2-propenoyl, 2-butenoyl, Isobutyryl, 2-methylpropanoyl, 2-ethylbutyryl, ethylbutanoyl, 2-ethylbutanoyl, butyryl,(E)-2,3-Dimethylacryloyl, (E)-2-Methylcrotonoyl,3-cis-Methyl-methacryloyl, 3-Methyl-2-butenoyl, 3-Methylcrotonoyl,4-Pentenoyl, (2E)-2-pentenoyl, Caproyl, 5-Hexenoyl, Capryloyl, Lauroyl,Dodecanoyl, Myristoyl, Tetradecanoyl, Oleoyl, C(2-18) Acyl, at apentacyclic triterpene, triterpene, triterpeniod, triterpeniod saponin,terpene, isoprene or compound selected from formulae of the presentapplication, produces inhbition of cancer growth, cancer invasion, cellsinvasion, cancer cell invasion, cell adhesion, cell circulation or cellattachment, blocking the DNA synthesis of cancer cell, increase thepotency and decrease the toxicity.

This invention shows that the presence of group selected from acetyl,angeloyl, tigloyl, senecioyl, Crotonoyl, 3,3-Dimethylartyloyl,Cinnamoyl, Pentenoyl, Hexanoyl, benzoyl, Ethylbutyryl, alkyl, dibenzoyl,benzoyl, methylbutanoyl, methylpropanoyl, alkanoyl, alkenoyl, benzoylalkyl substituted alkanoyl, alkanoyl substituted phenyl, alkenoylsubstituted phenyl, aryl, acyl, heterocylic, heteroraryl,alkenylcarbonyl, ethanoyl, propanoyl, propenoyl, butanoyl, butenoyl,pentanoyl, hexenoyl, heptanoyl, heptenoyl, octanoyl, octenoyl, nonanoyl,nonenoyl, decanoyl, decenoyl, propionyl, 2-propenoyl, 2-butenoyl,Isobutyryl, 2-methylpropanoyl, 2-ethylbutyryl, ethylbutanoyl,2-ethylbutanoyl, butyryl, (E)-2,3-Dimethylacryloyl,(E)-2-Methylcrotonoyl, 3-cis-Methyl-methacryloyl, 3-Methyl-2-butenoyl,3-Methylcrotonoyl, 4-Pentenoyl, (2E)-2-pentenoyl, Caproyl, 5-Hexenoyl,Capryloyl, Lauroyl, Dodecanoyl, Myristoyl, Tetradecanoyl, Oleoyl,C(2-18) Acyl at carbon position 21, 22, 24 and/or 28 of a pentacyclictriterpene, triterpene, triterpeniod, triterpeniod saponin or compoundselected from formulae of the present application, produces inhibitionof cancer growth, cancer invasion, cells invasion, cancer cell invasionor macromolecular cell invasion. In an embodiment, the presence ofgroup(s) selected from acetyl, angeloyl, tigloyl, senecioyl, Crotonoyl,3,3-Dimethylartyloyl, Cinnamoyl, Pentenoyl, Hexanoyl, benzoyl,Ethylbutyryl, alkyl, dibenzoyl, benzoyl, methylbutanoyl,methylpropanoyl, alkanoyl, alkenoyl, benzoyl alkyl substituted alkanoyl,alkanoyl substituted phenyl, alkenoyl substituted phenyl, aryl, acyl,heterocylic, heteroraryl, alkenylcarbonyl, ethanoyl, propanoyl,propenoyl, butanoyl, butenoyl, pentanoyl, hexenoyl, heptanoyl,heptenoyl, octanoyl, octenoyl, nonanoyl, nonenoyl, decanoyl, decenoyl,propionyl, 2-propenoyl, 2-butenoyl, Isobutyryl, 2-methylpropanoyl,2-ethylbutyryl, ethylbutanoyl, 2-ethylbutanoyl, butyryl,(E)-2,3-Dimethylacryloyl, (E)-2-Methylcrotonoyl,3-cis-Methyl-methacryloyl, 3-Methyl-2-butenoyl, 3-Methylcrotonoyl,4-Pentenoyl, (2E)-2-pentenoyl, Caproyl, 5-Hexenoyl, Capryloyl, Lauroyl,Dodecanoyl, Myristoyl, Tetradecanoyl, Oleoyl, C(2-18) Acyl at carbonposition 2, 3, 8, 15, 21, 22, 23, 24 and/or 28 of a pentacyclictriterpene, triterpeniod, triterpeniod saponin or compound selected fromformulae of the present application produces activities includinginhibition of cancer growth, cancer invasion, cells invasion, cancercell invasion, cell adhesion, cell attachment or cell circulatingwherein the group may attached with an O, S, NH, CH2O to the carbon oftriterpene, triterpeniod, triterpeniod saponin or compound selected fromformulae of the present application; wherein the group may be selectedfrom group of CH2O-angeloyl, CH2O-tigloyl, CH2O-senecioyl, CH2O-acetyl,CH2O-Crotonoyl, CH2O-3,3-Dimethylartyloyl, CH2O-Cinnamoyl,CH2O-Pentenoyl, CH2O-Hexanoyl, CH2O-benzoyl, CH2O-Ethylbutyryl, CH3,CH2OH, CH2O-alkyl, CH2O-dibenzoyl, CH2O-benzoyl, CH2O-alkanoyl,CH2O-alkenoyl, CH2O-benzoyl alkyl substituted O-alkanoyl, CH2O-alkanoylsubstituted phenyl, CH2O-alkenoyl substituted phenyl, CH2O-aryl,CH2O-acyl, CH2O-heterocylic, CH2O-heteroraryl, CH2O-alkenylcarbonyl,CH2O-ethanoyl, CH2O-propanoyl, CH2O-propenoyl, CH2O-butanoyl,CH2O-butenoyl, CH2O-pentanoyl, CH2O-hexenoyl, CH2O-heptanoyl,CH2O-heptenoyl, CH2O-octanoyl, CH2O-octenoyl, CH2O-nonanoyl,CH2O-nonenoyl, CH2O-decanoyl, CH2O-decenoyl, CH2O-propionyl,CH2O-2-propenoyl, CH2O-2-butenoyl, CH2O-Isobutyryl,CH2O-2-methylpropanoyl, CH2O-2-ethylbutyryl, CH2O-ethylbutanoyl,CH2O-2-ethylbutanoyl, CH2O-butyryl, CH2O-(E)-2,3-Dimethylacryloyl,CH2O-(E)-2-Methylcrotonoyl, CH2O-3-cis-Methyl-methacryloyl,CH2O-3-Methyl-2-butenoyl, CH2O-3-Methylcrotonoyl, CH2O-4-Pentenoyl,CH2O-(2E)-2-pentenoyl, CH2O-Caproyl, CH2O-5-Hexenoyl, CH2O-Capryloyl,CH2O-Lauroyl, CH2O-Dodecanoyl, CH2O-Myristoyl, CH2O-Tetradecanoyl,CH2O-Oleoyl, CH2O—C(2-18) Acyl, (CnH2n)O-angeloyl, (CnH2n)O-tigloyl,(CnH2n)O-senecioyl, (CnH2n)O-acetyl, (CnH2n)O-Crotonoyl,(CnH2n)O-3,3-Dimethylartyloyl, (CnH2n)O-Cinnamoyl, (CnH2n)O-Pentenoyl,(CnH2n)O-Hexanoyl, (CnH2n)O-benzoyl, (CnH2n)O-Ethylbutyryl,(CnH2n)O-alkyl, (CnH2n)O-dibenzoyl, (CnH2n)O-benzoyl, (CnH2n)O-alkanoyl,(CnH2n)O-alkenoyl, (CnH2n)O-benzoyl alkyl substituted O-alkanoyl,(CnH2n)O-alkanoyl substituted phenyl, (CnH2n)O-alkenoyl substitutedphenyl, (CnH2n)O-aryl, (CnH2n)O-acyl, (CnH2n)O-heterocylic,(CnH2n)O-heteroraryl, (CnH2n)O-alkenylcarbonyl, (CnH2n)O-alkane,(CnH2n)O-alkene and (CnH2n)O-sugar moiety, wherein n is 1 or 2 or 3 or 4or over 5. In an embodiment, the presence of group at carbon position24, produces activities. In an embodiment, the presence of group atcarbon position 24 and 28 produces activities. In an embodiment, thepresence of group at carbon position 24 and 21 produces activities. Inan embodiment, the presence of group at carbon position 24, 28 and 21,produces activities. In an embodiment, the presence of group at carbonposition 24, 28 and 22 produces activities. In an embodiment, thepresence of group at carbon position 24, 28 and 3 produces activities.In an embodiment, the presence of group at carbon position 24, and 3produces activities. In an embodiment, the presence of group at carbonposition 28 and 3 produces activities. In an embodiment, the presence ofgroup at carbon position 3 produces activities. In an embodiment, thepresence of group at carbon position 21 and 22 produces activities. Inan embodiment, the hemolytic activity of the compound is reduced. Inembodiment, the compound is attached a sugar moiety(ies), acidmoiety(ies) or alduronic acid. In an embodiment, the presence of groupat carbon position 1, produces activities. In an embodiment, thepresence of group at carbon position 2, produces activities. In anembodiment, the presence of group at carbon position 3, producesactivities. In an embodiment, the presence of group at carbon position4, produces activities. In an embodiment, the presence of group atcarbon position 5, produces activities. In an embodiment, the presenceof group at carbon position 6, produces activities. In an embodiment,the presence of group at carbon position 7, produces activities. In anembodiment, the presence of group at carbon position 8, producesactivities. In an embodiment, the presence of group at carbon position9, produces activities. In an embodiment, the presence of group atcarbon position 10, produces activities. In an embodiment, the presenceof group at carbon position 11, produces activities. In an embodiment,the presence of group at carbon position 12, produces activities. In anembodiment, the presence of group at carbon position 13, producesactivities. In an embodiment, the presence of group at carbon position14, produces activities. In an embodiment, the presence of group atcarbon position 15, produces activities. In an embodiment, the presenceof group at carbon position 16, produces activities. In an embodiment,the presence of group at carbon position 17, produces activities. In anembodiment, the presence of group at carbon position 18, producesactivities. In an embodiment, the presence of group at carbon position19, produces activities. In an embodiment, the presence of group atcarbon position 20, produces activities. In an embodiment, the presenceof group at carbon position 21, produces activities. In an embodiment,the presence of group at carbon position 22, produces activities. In anembodiment, the presence of group at carbon position 23, producesactivities. In an embodiment, the presence of group at carbon position24, produces activities. In an embodiment, the presence of group atcarbon position 25, produces activities. In an embodiment, the presenceof group at carbon position 26, produces activities. In an embodiment,the presence of group at carbon position 27, produces activities. In anembodiment, the presence of group at carbon position 28, producesactivities. In an embodiment, the presence of group at carbon position29, produces activities. In an embodiment, the presence of group atcarbon position 30, produces activities. In an embodiment, theactivities are for treating cancers, inhibition of cancer growth, cancerinvasion, cells invasion, cancer cell invasion; cell adhesion, cellattachment, cell circulating; for treating mad cow disease; treatingprion diseases; for inhibiting viruses; for preventing cerebral aging;for improving memory; improving cerebral functions; for curing enuresis,frequent micturition, urinary incontinence; dementia, Alzheimer'sdisease, autism, brain trauma, Parkinson's disease or other diseasescaused by cerebral dysfunctions or neurodegeneration; for treatingarthritis, rheumatism, poor circulation, arteriosclerosis, Raynaud'ssyndrome, angina pectoris, cardiac disorder, coronary heart disease,headache, dizziness, kidney disorder; cerebrovascular diseasea;inhibiting NF-kappa B activation; for treating brain edema, severe acuterespiratory syndrome, respiratory viral diseases, chronic venousinsufficiency, hypertension, chronic venous disease, oedema,inflammation, hemorrhoids, peripheral edema formation, varicose veindisease, flu, post traumatic edema and postoperative swelling; forinhibiting blood clots, for inhibiting ethanol absorption; for loweringblood sugar; for regulating adrenocorticotropin and corticosteronelevels. This invention provides a composition for Anti-MS,anti-aneurysm, anti-asthmatic, anti-oedematous, anti-inflammatory,anti-bradykinic, anti-capillarihemorrhagic, anti-cephalagic,anti-cervicobrachialgic, anti-eclamptic, anti-edemic, anti-encaphalitic,anti-epiglottitic, anti-exudative, anti-flu, anti-fracture,anti-gingivitic, anti-hematomic, anti-herpetic, anti-histaminic,anti-hydrathritic, anti-meningitic, antioxidant, anti-periodontic,anti-phlebitic, anti-pleuritic, anti-raucedo, anti-rhinitic,anti-tonsilitic, anti-ulcer, anti-varicose, anti-vertiginous,cancerostatic, corticosterogenic, diuretic, fungicide, hemolytic,hyaluronidase inhibitor, lymphagogue, natriuretic, pesticide, pituitarystimulant, thymolytic, vasoprotective, inhibiting leishmaniases,modulating adhesion or angiogenesis of cells, anti-parasitic; increasethe expression of the genes: ANGPT2, DDIT3, LIF and NFKB1Z, andmanufacturing an adjuvant composition and venotonic treatment. In anembodiment, the compound is arresting cells in the S-phase and blockingtheir entering into the G2/M phase of cell cycle. The compound blocksthe DNA synthesis of cancer cell.

This invention provides compounds for improving blood circulation;soothing stroke; preventing plaque formation and promote theirdissipated; improve blood viscosity; reducing cardiovascular; reducingcerebrovascular; reducing thrombosis, arteriosclerosis, coronary heartdisease, hypertension, diabetes, thrombocytopenia purpura, hemoptysis,hematemesis; treating blood in the stool, uterine bleeding, traumaticbleeding, abdominal irritation, swelling, fluttering, Blood circulation,swelling, pain; Treating bronchiectasis, tuberculosis and lung abscesscaused by too hemoptysis; reducing bleeding, antitussive, expectorantand analgesic effect, dilate blood vessels; reducing blood pressure andthe treatment of cerebral arteriosclerosis; elevating blood lipids andreducing cholesterol.

In an embodiment, the compound is arresting cells in the S-phase andblocking their entering into the G2/M phase of cell cycle. The compoundblocks the DNA synthesis of cancer cell. This application producesynthetic compounds increase the potency and decrease the toxicity.

In an embodiment, the presence of group at carbon position 1, reducesactivities. In an embodiment, the presence of group at carbon position2, reduces activities. In an embodiment, the presence of group at carbonposition 3, reduces activities. In an embodiment, the presence of groupat carbon position 4, reduces activities. In an embodiment, the presenceof group at carbon position 5, reduces activities. In an embodiment, thepresence of group at carbon position 6, reduces activities. In anembodiment, the presence of group at carbon position 7, reducesactivities. In an embodiment, the presence of group at carbon position8, reduces activities. In an embodiment, the presence of group at carbonposition 9, reduces activities. In an embodiment, the presence of groupat carbon position 10, reduces activities. In an embodiment, thepresence of group at carbon position 11, reduces activities. In anembodiment, the presence of group at carbon position 12, reducesactivities. In an embodiment, the presence of group at carbon position13, reduces activities. In an embodiment, the presence of group atcarbon position 14, reduces activities. In an embodiment, the presenceof group at carbon position 15, reduces activities. In an embodiment,the presence of group at carbon position 16, reduces activities. In anembodiment, the presence of group at carbon position 17, reducesactivities. In an embodiment, the presence of group at carbon position18, reduces activities. In an embodiment, the presence of group atcarbon position 19, reduces activities. In an embodiment, the presenceof group at carbon position 20, reduces activities. In an embodiment,the presence of group at carbon position 21, reduces activities. In anembodiment, the presence of group at carbon position 22, reducesactivities. In an embodiment, the presence of group at carbon position23, reduces activities. In an embodiment, the presence of group atcarbon position 24, reduces activities. In an embodiment, the presenceof group at carbon position 25, reduces activities. In an embodiment,the presence of group at carbon position 26, reduces activities. In anembodiment, the presence of group at carbon position 27, reducesactivities. In an embodiment, the presence of group at carbon position28, reduces activities. In an embodiment, the presence of group atcarbon position 29, reduces activities. In an embodiment, the presenceof group at carbon position 30, reduces activities. In an embodiment theactivities are included but not limited to hemolytic activity, canceractivity, arthritis, rheumatism, poor circulation, arteriosclerosis,Raynaud's syndrome, angina pectoris, cardiac disorder, coronary heartdisease, headache, dizziness, kidney disorder; soothing stroke; plaqueformation; cardiovascular; cerebrovascular; thrombosis,arteriosclerosis, coronary heart disease, hypertension, diabetes,thrombocytopenia purpura, hemoptysis, hematemesis; blood in the stool,uterine bleeding, traumatic bleeding, abdominal irritation, swelling,fluttering, swelling, pain; bronchiectasis, tuberculosis and lungabscess; bleeding, tussive, expectorant and analgesic effect, bloodpressure and cerebral arteriosclerosis; blood lipids and cholesterol.

This invention shows a method of synthesizing active compound byattaching functional group to a core compound, wherein the functionalgroup(s) comprises a group which is/are selected from ethanoyl,propanoyl, propenoyl, butanoyl, butenoyl, pentanoyl, pentenoyl,hexanoyl, hexenoyl, heptanoyl, heptenoyl, octanoyl, octenoyl, nonanoyl,nonenoyl, decanoyl, decenoyl, propionyl, 2-propenoyl, crotonoyl,2-butenoyl, Isobutyryl, methylpropanoyl, 2-methylpropanoyl,ethylbutyryl, 2-ethylbutyryl, ethylbutanoyl, 2-ethylbutanoyl, butyryl,(E)-2,3-Dimethylacryloyl, (E)-2-Methylcrotonoyl,3-cis-Methyl-methacryloyl, 3-Methyl-2-butenoyl, 3-Methylcrotonoyl,4-Pentenoyl, (2E)-2-pentenoyl, Caproyl, 5-Hexenoyl, Capryloyl, Lauroyl,Dodecanoyl, Myristoyl, Tetradecanoyl, Oleoyl, tigloyl, angeloyl, acetyl,crotonoyl, 3,3-Dimethylartyloyl, senecioyl, cinnamoyl, benzoyl,ethylbutyryl, dibenzoyl, alkanoyl, alkenoyl, benzoyl alkyl substitutedalkanoyl, alkanoyl substituted phenyl, alkenoyl substituted phenyl,aryl, acyl, heterocylic, and heteroraryl, wherein the core compound is atriperpene or 5 ring triterpene. In embodiment, the core compound is a 4ring terpene. In embodiment, the core compound is a 3 ring terpene. Inembodiment, the core compound is a 2 ring terpene. In embodiment, thecore compound is a 1 ring terpene. The compounds provided in theinvention are for treating cancers, inhibition of cancer growth, cancerinvasion, cells invasion, cancer cell invasion; cell adhesion, cellattachment, cell circulating; for treating mad cow disease; treatingprion diseases; for inhibiting viruses; for preventing cerebral aging;for improving memory; improving cerebral functions; for curing enuresis,frequent micturition, urinary incontinence; dementia, Alzheimer'sdisease, autism, brain trauma, Parkinson's disease or other diseasescaused by cerebral dysfunctions or neurodegeneration; for treatingarthritis, rheumatism, poor circulation, arteriosclerosis, Raynaud'ssyndrome, angina pectoris, cardiac disorder, coronary heart disease,headache, dizziness, kidney disorder; cerebrovascular diseasea;inhibiting NF-kappa B activation; for treating brain edema, severe acuterespiratory syndrome, respiratory viral diseases, chronic venousinsufficiency, hypertension, chronic venous disease, oedema,inflammation, hemorrhoids, peripheral edema formation, varicose veindisease, flu, post traumatic edema and postoperative swelling; forinhibiting blood clots, for inhibiting ethanol absorption; for loweringblood sugar; for regulating adrenocorticotropin and corticosteronelevels. This invention provides a composition for Anti-MS,anti-aneurysm, anti-asthmatic, anti-oedematous, anti-inflammatory,anti-bradykinic, anti-capillarihemorrhagic, anti-cephalagic,anti-cervicobrachialgic, anti-eclamptic, anti-edemic, anti-encaphalitic,anti-epiglottitic, anti-exudative, anti-flu, anti-fracture,anti-gingivitic, anti-hematomic, anti-herpetic, anti-histaminic,anti-hydrathritic, anti-meningitic, antioxidant, anti-periodontic,anti-phlebitic, anti-pleuritic, anti-raucedo, anti-rhinitic,anti-tonsilitic, anti-ulcer, anti-varicose, anti-vertiginous,cancerostatic, corticosterogenic, diuretic, fungicide, hemolytic,hyaluronidase inhibitor, lymphagogue, natriuretic, pesticide, pituitarystimulant, thymolytic, vasoprotective, inhibiting leishmaniases,modulating adhesion or angiogenesis of cells, anti-parasitic; increasethe expression of the genes: ANGPT2, DDIT3, LIF and NFKB1Z, andmanufacturing an adjuvant composition and venotonic treatment. In anembodiment, the compound is arresting cells in the S-phase and blockingtheir entering into the G2/M phase of cell cycle. The compound blocksthe DNA synthesis of cancer cell.

Experiments presented in this invention showed that the compound AKOHhas no effect in inhibiting cancer growth, cancer invasion, cellsinvasion or cancer cell invasion. AKOH was obtained by removing theangeloyl groups from carbon positions 21 and 22 of the active XanifoliaY(Y3). This invention shows that the ability for inhibiting cancerinvasion, cells invasion or cancer cell invasion of Xanifolia Y(Y3) arelost by removing angeloyl groups from carbon positions 21 and 22.

Experiments presented in this invention showed that the core compoundincluding E4A, E5A, Xanifolia Y-core have no effect in inhibiting cancergrowth, cancer invasion, cells invasion or cancer cell invasion.Xanifolia Y-core was obtained by removing the angeloyl groups fromcarbon positions 21 and 22, and the sugar moieties from carbon 3 of theactive Xanifolia Y(Y3). E4A (E IV A) was obtained by removing the groupsfrom carbon positions 3, 21 and 22 of the active Escin. E5A (E V A) wasobtained by removing the groups from carbon positions 3, 21 and 22 ofthe active Escin. This invention showed that the core compound includingE4A, E4AY2, E5A, Xanifolia Y-core and AKOH have no hemolytic activityand no anti-cancer activity.

This invention showed that functional group attached at carbon position24 of a pentacyclic triterpene did not produce hemolytic activity, whichhas bio-activities including inhibiting cancer growth, inhibiting cancerinvasion, cells invasion or cancer cell invasion. This invention showedthat functional group attached at carbon position 3 of a pentacyclictriterpene did not produce hemolytic activity, which has bio-activitiesincluding inhibiting cancer growth, inhibiting cancer invasion, cellsinvasion or cancer cell invasion. This invention showed that functiongroup(s) attached at carbon position 3 and 1 or 2 or 3 of carbonposition 28, 21, 22, 24 of a pentacyclic triterpene did not producehemolytic activity, which has bio-activities including inhibiting cancergrowth, inhibiting cancer invasion, cells invasion or cancer cellinvasion.

This invention showed that functional group attached at carbon position2 of a pentacyclic triterpene did not produce hemolytic activity, whichhas bio-activities including inhibiting cancer growth, inhibiting cancerinvasion, cells invasion or cancer cell invasion. This invention showedthat function group(s) attached at carbon position 2 and 1 or 2 or 3 ofcarbon position 28, 21, 22, 24 of a pentacyclic triterpene did notproduce hemolytic activity, which has bio-activities includinginhibiting cancer growth, inhibiting cancer invasion, cells invasion orcancer cell invasion.

This invention provides a pentacyclic triterpene with reduced hemolyticactivity for treating diseases, wherein the triterpene comprising agroup(s) attached at its core producing bio-activities. This inventionprovides a pentacyclic triterpene with reduced hemolytic activitycomprising a group(s) attached at carbon position 3, or carbon position24 and 1 or 2 or 3 of other position(s) of a pantacyclic triterpene,which has bio-activities. This invention provides a triterpene withreduced hemolytic activity comprising a group(s) attached at carbonposition 15, 16, 21, 22, 23, 24, 28, 29, 30 and/or 3 of a pentacyclictriterpene, which has bio-activities. This invention provides acomposition comprising a triterpene with reduced hemolytic activitycomprising a group(s) attached at carbon position 3, or carbon position24 and 1 or 2 or 3 of other position(s) of a pentacyclic triterpene,which has bio-activities. This invention provides a method forbio-ativities treatment including but not limited to treating cancers,comprising administering to said subject an effective amount ofcompound, wherein the compound is a pentacyclic triterpene with reducedhemolytic activity comprising a group(s) attached at carbon position 3,or carbon position 24 and 1 or 2 or 3 of other position(s) of apentacyclic triterpene, which has bio-activities.

The compound of the present application can be obtained with the method:

-   -   1. Dissolving core compound or triterpenes, hydroxylated        triterpenes core in pyridine,    -   2. Adding acyl chloride,    -   3. The mixture is stirred for length of time including 5 sec, 10        sec, 20 sec, 30 sec, 40 sec, 1 min, 2 min, 5 min, 10 min, 30        min, 1 hr, 2 hr, 18 hr, 2 days or 3 days at 0 C, 25 C, 50 C or        75 C,    -   4. At the end of reaction, an aqueous solution of acid or base,        or water is added to the reaction mixture,    -   5. The solution is then extracted of ethyl acetate and ethyl        acetate is removed by evaporation and lyophilization,    -   6. Dissolving the reaction product in acetonitrile with        Trifluoroacetic acid or DMSO,    -   7. Testing the reaction product of mixtures and individual        fractions with MTT cytotoxic assay,    -   8. Selecting the HPLC fractions for isolation is according to        the cytotoxic activity of the reaction product obtained at a        specific reaction time,    -   9. Purifiing the active esterification products with HPLC,    -   10. Collecting the products,    -   11. Testing the products.

The compound of present application, wherein the core compound isterpene, isoprene, or triterpene core; wherein the core compound ishydroxylated; wherein the core compound was dissolved in pyridine;wherein the acyl chloride including Tigloyl chloride, angeloyl chloride,Acetyl chloride, Crotonoyl chloride, 3,3-Dimethylartyloyl chloride,senecioyl chloride, Cinnamoyl chloride, Pentenoyl chloride, Hexanoylchloride, benzoyl chloride and Ethylbutyryl chloride; wherein thereaction time for the mixture is stirred for 5 sec, 1 min, 2 min, 5 min,10 min, 30 min, 1 hr, 2 hr, 18 hr, 2 days or 3 days; wherein thetemperature is 0 C, 25 C, 50 C or 75 C temperature; wherein the acidincluding HCl or the base is a weak base including NaHCO3 is added tothe reaction mixture; wherein the solution is then extracted 3 timeswith ethyl acetate and lyophilization; wherein the reaction product isdissolved in 80% acetonitrile—0.005% Trifluoroacetic acid or DMSO;wherein selecting the HPLC fractions for isolation is according to thecytotoxic activity of the reaction product obtained at a reaction timeof 5 sec, 1 min, 2 min, 5 min, 10 min, 30 min, 1 hr, 2 hr, 18 hr, 2 daysor 3 days.

This invention showed that functional group attached at carbon position23 of a pentacyclic triterpene did not produce hemolytic activity, whichhas bio-activities including inhibiting cancer growth, inhibiting cancerinvasion, cells invasion or cancer cell invasion. This invention showedthat function group(s) attached at carbon position 24 and 1 or 2 or 3 ofcarbon position 28, 21, 22 of a pentacyclic triterpene did not producehemolytic activity, which has bio-activities including inhibiting cancergrowth, inhibiting cancer invasion, cells invasion or cancer cellinvasion.

This invention showed that functional group attached at carbon position24 of a pentacyclic triterpene did not produce hemolytic activity, whichhas bio-activities including inhibiting cancer growth, inhibiting cancerinvasion, cells invasion or cancer cell invasion. This invention showedthat function group(s) attached at carbon position 24 and 1 or 2 or 3 ofcarbon position 28, 21, 22 of a pentacyclic triterpene did not producehemolytic activity, which has bio-activities including inhibiting cancergrowth, inhibiting cancer invasion, cells invasion or cancer cellinvasion.

This invention provides a triterpene with reduced hemolytic activity fortreating diseases, wherein the triterpene comprising a group(s) attachedat its core producing bio-activities. This invention provides apentacyclic triterpene with reduced hemolytic activity comprising agroup(s) attached at carbon position 24, or carbon position 24 and 1 or2 or 3 of other position(s) of a pentacyclic triterpene, which hasbio-activities. This invention provides a composition comprising apentacyclic triterpene with reduced hemolytic activity comprising agroup(s) attached at carbon position 24, or carbon position 24 and 1 or2 or 3 of other position(s) of a pentacyclic triterpene, which hasbio-activities. This invention provides a method for bio-ativitiestreatment including but not limited to treating cancers, comprisingadministering to said subject an effective amount of compound, whereinthe compound is a triterpene with reduced hemolytic activity comprisinga group(s) attached at carbon position 24, or carbon position 24 and 1or 2 or 3 of other position(s) of a pentacyclic triterpene, which hasbio-activities.

This invention showed that Tig-N, Tig-Q, Tig-R, Tig-T Tig-S and Tig-V donot have hemolytic activity up to 20 ug/ml. The original compound ESlyse 100% red blood cells (RBC) at 5 ug/ml. Compare to Y3, the ACH-Y3 isless potent in hemolytic activity. Tig-R has no hemolytic activity. Thisinvention showed that Tig-N, Tig-Q, Tig-R, Tig-T Tig-S and Tig-V haveanti cancer activities.

Many saponins and triterpenes have hemolytic characteristic that damagered blood cells. This severe side effect make people hesitate to usesaponins or triterpenes in medicines. This invention produces sythesisedsaponins and triterpenes with reduced hemolytic characteristic for useas medicament. This invention produces compounds with reduced hemolyticcharacteristic for use as medicament. The medicament can be used fortreating cancer, inhibiting cancer growth, cancer invasion, cellsinvasion or cancer cell invasion. This application produce syntheticcompounds increase the potency and decrease the toxicity.

This invention shows that the ability for inhibiting cancer growth,cancer invasion, cells invasion or cancer cell invasion are maintainedwhen the sugar moieties are removed from carbon position 3 of an activecompound, triterpene, triterpeniod, or triterpeniod saponin. Experimentspresented in this invention showed that the compound ACH-Y3 has theability to inhibit cancer invasion, cells invasion or cancer cellinvasion. The compound ACH-Y3 was obtained by removing the sugarmoieties from carbon position 3 of a active Xanifolia Y(Y3). Thisinvention shows that the ability for inhibiting cancer invasion, cellsinvasion or cancer cell invasion are maintained when the sugar moietiesare removed from the carbon position 3 of active Xanifolia Y(Y3).

A compound which has bio-activities including inhibiting cancer growth,inhibiting cancer invasion, cells invasion or cancer cell invasion iscalled active compound.

This invention provides a use for compounds, compositions, and methodsfor manufacturing medicament for treating cancers, inhibition of cancergrowth, cancer invasion, cells invasion, cancer cell invasion; celladhesion, cell attachment, cell circulating, or for inhibiting cancermetastasis, wherein the compounds comprise the structures selected fromthe formulae of the present application, wherein the compounds can besynthesized or isolated, wherein the compounds comprise the pentacyclictriterpenes, wherein the compounds with reduced hemolytic, wherein thecells comprise cancer cells, wherein the cancers comprise breast cancer,leukocytic cancer, liver cancer, ovarian cancer, bladder cancer,prostatic cancer, skin cancer, bone cancer, brain cancer, leukemiacancer, lung cancer, colon cancer, CNS cancer, melanoma cancer, renalcancer, cervical cancer, esophageal cancer, testicular cancer, spleeniccancer, kidney cancer, lymphhatic cancer, pancreatic cancer, stomachcancer and thyroid cancer. The method of inhibiting cancer invasion,cells invasion or cancer cell invasion activities uses non-cytotoxicdrug concentrations. The method of inhibiting metastasis usesnon-cytotoxic drug concentrations. There is no noticeable change in cellmorphology.

This invention provides triterpene(s) with reduced hemolytic activityfor treating diseases, wherein the triterpene can be a pentacyclictriterpene comprising a group(s) attached at its core producingbio-activities. This invention provides a pentacyclic triterpene withreduced hemolytic effect, comprising a group(s) attached at carbonposition 24, or carbon position 24 and 1 or 2 or 3 of other position(s)of a pentacyclic triterpene, which has bio-activities. This inventionprovides a composition comprising a triterpene with reduced hemolyticactivity comprising a group(s) attached at carbon position 24, or carbonposition 24 and 1 or 2 or 3 of other position(s) of a pentacyclictriterpene, which has bio-activities. This invention provides a methodfor bio-ativities treatment including but not limited to treatingcancers, comprising administering to said subject an effective amount ofcompound, wherein the compound is a triterpene with reduced hemolyticactivity, comprising a group(s) attached at carbon position 24, orcarbon position 24 and 1 or 2 or 3 of other position(s) of a triterpene,which has bio-activities, wherein a compound selected from A1-18,A20-32, B1-18, B20-32, C1-18, C20-32, D1-18, D20-32, D1-18, D20-32,D1-18, D20-32, D1-18, D20-32, D1-18, D20-32, E1-18, E20-32, G1-18,G20-32, H1-18, H20-32, 11-18, 120-32, J1-18, J20-32, K1-18, K20-32,Tig-Sen-n, Tig-Cro-n, Tig-Acy-n, Tig-Pen-n, Tig-Hex-n, Tig-Cin-n,Tig-Ang-n, Tig-Eth-n, Tig-R-Sen-n, Tig-R-Cro-n, Tig-R-Acy-n,Tig-R-Pen-n, Tig-R-Hex-n, Tig-R-Cin-n, Tig-R-Ang-n, Tig-R-Eth-n, whereinn=1 to 6, and a salt, ester, metabolite thereof, and the compoundsselected from formulae 2A, and K; wherein the compound is selected fromTig-N, Tig-Q, Tig-R, Tig-T Tig-S and Tig-V.

This invention provides methods for treating cancers, inhibition ofcancer growth, cancer invasion, cells invasion, cancer cell invasion;cell adhesion, cell attachment, cell circulating, migration, metastasisor growth of cancers, wherein the methods comprise affecting geneexpression, wherein the methods comprise stimulating gene expression, orwherein the methods comprise inhibiting the gene expression, or whereinthe methods comprise administering to a subject an effective amount ofcompounds, compositions in this application. In an embodiment, themethod comprises contacting said cell with a compound selected fromA1-18, A20-32, B1-18, B20-32, C1-18, C20-32, D1-18, D20-32, D1-18,D20-32, D1-18, D20-32, D1-18, D20-32, D1-18, D20-32, E1-18, E20-32,G1-18, G20-32, H1-18, H20-32, 11-18, 120-32, J1-18, J20-32, K1-18,K20-32, Xanifolia Y0, Y1, Y2, Y(Y3), Y5, Y7, Y8, Y9, Y10, Xanifolia (x),M10, Escin(bES), Aescin, ACH-Y(Y3), ACH-Y10, ACH-Y2, ACH-Y8, ACH-Y7,ACH-Y0, ACH-X, ACH-Z4, ACH-Z1, ACH-Escin(bES), ACH-M10, Tig-Sen-n,Tig-Cro-n, Tig-Acy-n, Tig-Pen-n, Tig-Hex-n, Tig-Cin-n, Tig-Ang-n,Tig-Eth-n, Tig-R-Sen-n, Tig-R-Cro-n, Tig-R-Acy-n, Tig-R-Pen-n,Tig-R-Hex-n, Tig-R-Cin-n, Tig-R-Ang-n, Tig-R-Eth-n, wherein n=1 to 6,and a salt, ester, metabolite thereof, and the compounds selected fromformulae 2A, and K. In vitro studies show that a compound selected fromstructure (2A) or (K) inhibits cell adhesion to culture flasks. Thecompound blocks the function of these adhesive molecules on cells. In anembodiment, the selected compound blocks the function of these adhesivemolecules on cells. In an embodiment, the selected compound blocks thefunction of these adhesive molecules on carcinoma cells. In anembodiment, the selected compound blocks the function of these adhesivemolecules on the mesothelial cells. This invention provides an antiadhesion therapy which uses the compound as a mediator or inhibitor ofadhesion proteins and angiopoietins. It inhibits excess adhesion andinhibits cell attachment. This invention provides compounds for use as amediator for cell circulating, cell moving and inflammatory diseases. Inan embodiment, the selected compound binds to the adhesive proteins (bymasking) on the membrane and inhibits the interaction of adhesionproteins with their receptors. In an embodiment, the selected compound'saction on the membrane affects adhesion proteins' function in themembrane. The lost of adhesion activity of cancer cells is result fromdirect or indirect action of the selected compound on membrane proteins.(Our purification methods and biological assays include the MTT assay inInternational Application No. PCT/US05/31900, filed Sep. 7, 2005, U.S.Ser. No. 11/289,142, filed Nov. 28, 2005, and U.S. Ser. No. 11/131,551,filed May 17, 2005, and PCT/US2008/002086, 1188-ALA-PCT, filed Feb. 15,2008, the cell invasion experiments methods in International ApplicationPCT/US2010/0042240, filed Jul. 16, 2010)

This invention provides a use of compounds or methods for inhibitingcancer invasion, cell invasion, cancer cell invasion, macromolecularcell invasion, migration, metastasis or growth of cancers, wherein thisinvention comprises a process and method for administration of thecomposition, wherein administration is by intravenous injection,intravenous drip, intraperitoneal injection or oral administration;wherein administration is by intravenous drip: 0.003-0.03 mg/kg bodyweight of compound dissolved in 250 ml of 10% glucose solution or in 250ml of 0.9% NaCl solution, or by intravenous injection: 0.003-0.03 mg/kgbody weight per day of compound dissolved in 10-20 ml of 10% glucosesolution or of 0.9% NaCl solution, or 0.01-0.03 mg/kg body weight ofcompound dissolved in 250 ml of 10% glucose solution or in 250 ml of0.9% NaCl solution, or by intravenous injection: 0.01-0.03 mg/kg bodyweight per day of compound dissolved in 10-20 ml of 10% glucose solutionor of 0.9% NaCl solution, or 0.01-0.05 mg/kg body weight of compounddissolved in 250 ml of 10% glucose solution or in 250 ml of 0.9% NaClsolution, or by intravenous injection: 0.01-0.05 mg/kg body weight perday of compound dissolved in 10-20 ml of 10% glucose solution or of 0.9%NaCl solution, or 0.05-0.2 mg/kg body weight of compound dissolved in250 ml of 10% glucose solution or in 250 ml of 0.9% NaCl solution, or byintravenous injection: 0.05-0.2 mg/kg body weight per day of compounddissolved in 10-20 ml of 10% glucose solution or of 0.9% NaCl solution,or by intravenous drip: 0.1-0.2 mg/kg body weight per day of compounddissolved in 250 ml of 10% glucose solution or in 250 ml of 0.9% NaClsolution, or by intravenous injection: 0.1-0.2 mg/kg body weight per daycompound dissolved in 10-20 ml of 10% glucose solution or of 0.9% NaClsolution, or by intraperitoneal injection (I.P.): 2.5 mg/kg body weightper day compound dissolved in 10% glucose solution or of 0.9% NaClsolution, or by oral administration wherein the dosage of mammal is 1-10mg/kg, 10-30 mg/kg, 30-60 mg/kg, or 60-90 mg/kg body weight of compound,or by intravenous injection or intravenous drip wherein the dosage ofmammal is 0.01-0.1 mg/kg body weight, 0.1-0.2 mg/kg, 0.2-0.4 mg/kg bodyweight, or 0.4-0.6 mg/kg body weight of compound, or by intraperitonealinjection (I.P.) wherein the dosage of mammal is 1-3 mg/kg, 3-5 mg/kg,4-6 mg/kg, or 6-10 mg/kg body weight of compound, or 10-50 mg/kg bodyweight of compound, or 50-100 mg/kg body weight of compound, or 30-70mg/kg body weight of compound or 100-150 mg/kg body weight of compound.

This invention provides a use of compounds or methods for treatingcancers, inhibition of cancer growth, cancer invasion, cells invasion,cancer cell invasion; macromolecular cell invasion, cell adhesion, cellattachment, cell circulating, migration, metastasis or growth ofcancers, infection or re-infection of virus or infectiousmacromolecules, and cancer cell fusion, wherein the invention comprisesa pharmaceutical composition comprising the compound of this inventionor a pharmaceutically acceptable salt thereof, and a pharmaceuticallyacceptable carrier or diluent, wherein said compound is present in aconcentration of 0.01 ug/ml to 65 ug/ml, or wherein said compound ispresent in a concentration of 0.01 ug/ml to 40 ug/ml, or wherein saidcompound is present in a concentration of 0.01 ug/ml to 30 ug/ml, orwherein said compound is present in a concentration of 0.01 ug/ml to 10ug/ml, or wherein said compound is present in a concentration of 0.01ug/ml to 5 ug/ml, or wherein said compound is present in a concentrationof 5 ug/ml to 10 ug/ml, or wherein said compound is present in aconcentration of 0.1 ug/ml to 5 ug/ml, or wherein said compound ispresent in a concentration of 0.1 ug/ml to 7.5 ug/ml, or wherein saidcompound is present in a concentration of 0.1 ug/ml to 10 ug/ml, orwherein said compound is present in a concentration of 0.1 ug/ml to 15ug/ml, or wherein said compound is present in a concentration of 0.1ug/ml to 20 ug/ml, or wherein said compound is present in aconcentration of 0.1 ug/ml to 30 ug/ml, or wherein said compound ispresent in a concentration of 1 ug/ml to 5 ug/ml, or wherein saidcompound is present in a concentration of 1 ug/ml to 7.5 ug/ml, orwherein said compound is present in a concentration of 1 ug/ml to 10ug/ml, or wherein said compound is present in a concentration of 1 ug/mlto 15 ug/ml, or wherein said compound is present in a concentration of 1ug/ml to 20 ug/ml, or wherein said compound is present in aconcentration of 1 ug/ml to 30 ug/ml, or wherein said compound ispresent in a concentration of 3 ug/ml to 5 ug/ml, or wherein saidcompound is present in a concentration of 3 ug/ml to 7.5 ug/ml, orwherein said compound is present in a concentration of 3 ug/ml to 10ug/ml, or wherein said compound is present in a concentration of 3 ug/mlto 15 ug/ml, or wherein said compound is present in a concentration of 3ug/ml to 20 ug/ml, or wherein said compound is present in aconcentration of 3 ug/ml to 30 ug/ml, or wherein said compound ispresent in a concentration of 4 ug/ml to 5 ug/ml, or wherein saidcompound is present in a concentration of 4 ug/ml to 7.5 ug/ml, orwherein said compound is present in a concentration of 4 ug/ml to 10ug/ml, or wherein said compound is present in a concentration of 4 ug/mlto 15 ug/ml, or wherein said compound is present in a concentration of 4ug/ml to 20 ug/ml, or wherein said compound is present in aconcentration of 4 ug/ml to 30 ug/ml, or wherein said compound ispresent in a concentration of 5 ug/ml to 8 ug/ml, or wherein saidcompound is present in a concentration of 5 ug/ml to 9 ug/ml, or whereinsaid compound is present in a concentration of 5 ug/ml to 10 ug/ml, orwherein said compound is present in a concentration of 5 ug/ml to 15ug/ml, or wherein said compound is present in a concentration of 5 ug/mlto 20 ug/ml, or wherein said compound is present in a concentration of 5ug/ml to 30 ug/ml, or wherein said compound is present in aconcentration of 7 ug/ml to 8 ug/ml, or wherein said compound is presentin a concentration of 7 ug/ml to 9 ug/ml, or wherein said compound ispresent in a concentration of 7 ug/ml to 10 ug/ml, or wherein saidcompound is present in a concentration of 7 ug/ml to 15 ug/ml, orwherein said compound is present in a concentration of 7 ug/ml to 20ug/ml, or wherein said compound is present in a concentration of 7 ug/mlto 30 ug/ml, or wherein said compound is present in a concentration of30 ug/ml to 70 ug/ml or wherein said compound is present in aconcentration of 70 ug/ml to 100 ug/ml or wherein said compound ispresent in a concentration of 100 ug/ml to 150 ug/ml.

This invention provides a use of compounds or methods for treatingcancers, inhibition of cancer growth, cancer invasion, cells invasion,cancer cell invasion; macromolecular cell invasion, cell adhesion, cellattachment, cell circulating, migration, metastasis or growth ofcancers, infection or re-infection of virus or infectiousmacromolecules, and cancer cell fusion, wherein the invention comprisesa pharmaceutical composition comprising the compound of this inventionor a pharmaceutically acceptable salt thereof, and a pharmaceuticallyacceptable carrier or diluent, wherein said compound is present in aconcentration of 0.008 uM to 80 uM, or wherein said compound is presentin a concentration of 0.01 uM to 60 uM, or wherein said compound ispresent in a concentration of 0.01 uM to 50 uM, or wherein said compoundis present in a concentration of 0.01 uM to 40 uM, or wherein saidcompound is present in a concentration of 0.01 uM to 30 uM, or whereinsaid compound is present in a concentration of 0.01 uM to 20 uM, orwherein said compound is present in a concentration of 0.01 uM to 10 uM,or wherein said compound is present in a concentration of 5 uM to 10 uM,or wherein said compound is present in a concentration of 0.1 uM to 5uM, or wherein said compound is present in a concentration of 0.1 uM to7.5 uM, or wherein said compound is present in a concentration of 0.1 uMto 10 uM, or wherein said compound is present in a concentration of 0.1uM to 15 uM, or wherein said compound is present in a concentration of0.1 uM to 20 uM, or wherein said compound is present in a concentrationof 0.1 uM to 30 uM or wherein said compound is present in aconcentration of 0.1 uM to 40 uM, or wherein said compound is present ina concentration of 0.1 uM to 50 uM or wherein said compound is presentin a concentration of 0.1 uM to 60 uM, or wherein said compound ispresent in a concentration of 0.1 uM to 80 uM, or wherein said compoundis present in a concentration of 1 uM to 5 uM, or wherein said compoundis present in a concentration of 1 uM to 7.5 uM, or wherein saidcompound is present in a concentration of 1 uM to 10 uM, or wherein saidcompound is present in a concentration of 1 uM to 15 uM, or wherein saidcompound is present in a concentration of 1 uM to 20 uM, or wherein saidcompound is present in a concentration of 1 uM to 30 uM or wherein saidcompound is present in a concentration of 1 uM to 40 uM, or wherein saidcompound is present in a concentration of 1 uM to 50 uM or wherein saidcompound is present in a concentration of 1 uM to 60 uM, or wherein saidcompound is present in a concentration of 1 uM to 80 uM, or wherein saidcompound is present in a concentration of 3 uM to 5 uM, or wherein saidcompound is present in a concentration of 3 uM to 7.5 uM, or whereinsaid compound is present in a concentration of 3 uM to 10 uM, or whereinsaid compound is present in a concentration of 3 uM to 15 uM, or whereinsaid compound is present in a concentration of 3 uM to 20 uM, or whereinsaid compound is present in a concentration of 3 uM to 30 uM or whereinsaid compound is present in a concentration of 3 uM to 40 uM, or whereinsaid compound is present in a concentration of 3 uM to 50 uM or whereinsaid compound is present in a concentration of 3 uM to 60 uM, or whereinsaid compound is present in a concentration of 3 uM to 80 uM, or whereinsaid compound is present in a concentration of 5 uM to 8 uM, or whereinsaid compound is present in a concentration of 5 uM to 10 uM, or whereinsaid compound is present in a concentration of 5 uM to 15 uM, or whereinsaid compound is present in a concentration of 5 uM to 20 uM, or whereinsaid compound is present in a concentration of 5 uM to 30 uM or whereinsaid compound is present in a concentration of 5 uM to 40 uM, or whereinsaid compound is present in a concentration of 5 uM to 50 uM or whereinsaid compound is present in a concentration of 5 uM to 60 uM, or whereinsaid compound is present in a concentration of 5 uM to 80 uM. or whereinsaid compound is present in a concentration of 7 uM to 8 uM, or whereinsaid compound is present in a concentration of 7 uM to 10 uM, or whereinsaid compound is present in a concentration of 7 uM to 15 uM, or whereinsaid compound is present in a concentration of 7 uM to 20 uM, or whereinsaid compound is present in a concentration of 7 uM to 30 uM or whereinsaid compound is present in a concentration of 7 uM to 40 uM, or whereinsaid compound is present in a concentration of 7 uM to 50 uM or whereinsaid compound is present in a concentration of 7 uM to 60 uM, or whereinsaid compound is present in a concentration of 7 uM to 80 uM or whereinsaid compound is present in a concentration of 70 uM to 100 uM, orwherein said compound is present in a concentration of 90 uM to 120 uM.

The invention will be better understood by reference to the ExperimentalDetails which follow, but those skilled in the art will readilyappreciate that the specific experiments detailed are only illustrative,and are not meant to limit the invention as described herein, which isdefined by the claims which follow thereafter.

Throughout this application, various references or publications arecited. Disclosures of these references or publications in theirentireties are hereby incorporated by reference into this application inorder to more fully describe the state of the art to which thisinvention pertains.

It is to be noted that the transitional term “comprising”, which issynonymous with “including”, “containing” or “characterized by”, isinclusive or open-ended and does not exclude additional, un-recitedelements or method steps.

Example 1 Tablet for Dose Containing 10 mg, 20 mg 30 mg of ActiveCompound

Active compound  1 mg   5 mg   10 mg   20 mg  30 mg Microcrystalline 20mg   20 mg 19.75 mg   60 mg 100 mg cellulose Corn starch 29 mg 24.5 mg19.75 mg 19.25 mg 18.5 mg  Magnesium  0 mg  0.5 mg  0.5 mg  0.75 mg  1.5mg stearate

The active compound, cellulose, and a portion of the corn starch aremixed and granulated to 10% corn starch paste. The resulting granulationis sieved, dried and blended with the remainder of the corn starch andthe magnesium stearate. The resulting granulation is then compressedinto tablets containing 1, 5, 10, 20, 30 mg, respectively of activeingredient per tablet.

Example 2 Intravenous Solution Preparation

An intravenous dosage form of the active compound is prepared asfollows:

Active compound 1-10 ug

Sodium citrate 5-50 mg

Citric acid 1-15 mg

Sodium chloride 1-8 mg

Water for injection (USP) q.s. to 1 mL

Utilizing the above quantities, the active compound is dissolved at roomtemperature in a prepared solution of sodium chloride, citric acid, andsodium citrate in water for injection.

Example 3 Intravenous Drip Preparation

0.25-2.5 mg compound dissolved in 250 ml of 10% glucose solution or in250 ml of 0.9% NaCl solution.

Intravenous drip preparation: 1-2 mg compound dissolved in 250 ml of 10%glucose solution or in 250 ml of 0.9% NaCl solution

Treatment of angelic acid with one of the many standard chlorinatingreagents including phosphorus ocychloride, phosphorus trichloride andthionyl chloride produces tigloyl chloride. Oxalyl chloride produces a2:1 ratio of angeloyl chloride to tigloyl chloride. Treatment ofpotassium salt in diethyl ether with oxalyl chloride and catalytic DMFfor 2 hr at 0 C produces pure angeloyl chloride.

Acid Hydrolysis of the Following Compounds:

a) Xanifolia(Y),

or chemical name: 3-O-[β-D-galactopyranosyl (1→2)]-α-L-arabinofuranosy(1→3)-β-D-glucuronopyranosyl-21,22-O-diangeloyl-3β, 15α, 16α, 21β, 22α,28-hexahydroxyolean-12-ene;

c) Xanifolia (Y2),

or chemical name: 3-O-[β-D-glucopyranosyl-(1→2)]-α-L-arabinofuranosy(1→3)-β-D-glucuronopyranosyl-21,22-O-diangeloyl-3β, 15α, 16α, 21β, 22α,24β, 28-heptahydroxyolean-12-ene;

d) Xanifolia (Y8),

or chemical name: 3-O-[β-glucopyranosyl (1→2)]-α-arabinofuranosyl(1→3)-β-glucuronopyranosyl-21, 22-O-diangeloyl-3β, 16α, 21β, 22α, 24β,28-hexahydroxyolean-12-ene;

f) Xanifolia (Y10),

or chemical name: 3-O-[β-galactopyranosyl (1→2)]-α-arabinofuranosyl(1→3)-β-glucuronopyranosyl-21, 22-O-diangeloyl-3β, 16α, 21β, 22α,28-pentahydroxyolean-12-ene.

j) structure (M10)

m) structure (bES):

n)

p)

After acid hydrolysis of the above, an isolated, purified or synthesizedcompound is produced having a structure (ACH) selected from following:

The composition comprises bioactive compounds from natural plants orsynthesis.

The program is based on our purification methods and biological assaysincluding the MTT assay. See International Application No.PCT/US05/31900, filed Sep. 7, 2005, U.S. Ser. No. 11/289,142, filed Nov.28, 2005, and U.S. Ser. No. 11/131,551, filed May 17, 2005, andPCT/US2008/002086, 1188-ALA-PCT, filed Feb. 15, 2008, Ser. No.12/344,682, 1020-B1-US, filed Dec. 29, 2008. The details of Analysis ofgene expression of ES2 cells after Y-treatment by Microarray, DataAnalysis Methods and Western blot in PCT/US2008/002086, 1188-ALA-PCT,filed Feb. 15, 2008, and the cell invasion experiments methods inInternational Application PCT/US2010/0042240, filed Jul. 16, 2010.

The Haemolytic Assay

Erythrocytes (RBC) were isolated from human blood (EDTA whole blood,collected randomly). 50 ul of the 10% RBC suspension (in PBS) was addedto 2 ml of sample solutions (concentration range from 0.1 ug/ml to 400ug/ml) in PBS. The mixture was vortexed briefly and sat for 60 min atroom temperature. The mixture was spun at 3K for 10 min and the relativeamounts of lysed hemoglobin in the supernatant were measured at 540 nm.The synthetic compounds of present application were tested with thismethod.

Acid Hydrolysis of Saponin

15 mg Xanifolia-Y was dissolved in 1 ml of methanol. 1 ml of 2N HCl wasthen added. The mixture was refluxed in 80 C water bath for 5 hours. Thesolution was then neutralized by adding 2 ml of 1N NaOH (to final pH4-6). The aglycone was then extracted with ethylacetate 3 ml×2. Theextracts were collected and pooled. Further isolation of aglycone(ACH-Y) was achieved by HPLC with isocratic elution of 80-100%acetonitrile. Repeating the experiment with compounds Z4, Y10, Y2, Y8,Y7, Y0, X, M10 and ESCIN (bES) gives the following compoundsrespectively: ACH-Z4, ACH-Y10, ACH-Y2, ACH-Y8, ACH-Y7, ACH-Y0, ACH-X,ACH-E, ACH-Z5, ACH-M10 and ACH-bES.

Removal of the Acyl Group by Alkaline Hydrolysis

20 mg of Xanifolia-Y was dissolved in 0.5 ml of 1N NaOH. The solutionwas incubated in 80 C water bath for 4 hours. It was cooled to roomtemperature before being neutralized with 0.5 ml 1N HCl (adjust pH toabout 3). The mixture was extracted with 2 ml 1-butanol 3 times. Thebutanol fractions were collected and lyophilized. The hydrolyzed saponinwas further purified with HPLC in a C-18 column eluted with 25%acetonitrile.

Compounds AKOH-Y and AKOH-M10 do not show the ability to inhibit cancergrowth, cancer invasion, cells invasion or cancer cell invasion.

Core Compound

A core compound or pentacyclic triterpenes, hydroxylated triterpenes isobtained by acid and alkaline hydroysis of saponin from natural sources.A pentacyclic triterpene can also be obtained by synthetic methods. Amethod for synthesizing the core compound is as follows:

Beta-Escin, compound Y, Y10, Y2, Y8, Y7, Y0, X, or M10 dissolved in 1MNaOH (20 mg/ml) was incubated at 70 C for 5 hours. The hydrolyzedsolution was neutralized with HCl and the water was evaporated bylyophilization. The product was dissolved in 50% methanol and 1N HCl.The mixture was incubated at 70 C for 5 hours. The solution wasneutralized with NaOH. The hydrolyzed product was extracted withethylacetate, which was subsequently removed by evaporation. Furtherpurification of the hydrolyzed product of core compounds including (E4A)were archived with FPLC chromatography in a C18 column equilibrated with70% acetonitrile/TFA at the flow rate of 1 ml/min. The core compoundsare obtained.

The core compounds do not show the ability to inhibit cancer growth,cancer invasion, or cell adhesion. The structures of core compounds:

wherein R1, R2, R5, R8 represent OH; R3 represents OH, H or absent; R4,R10 represent CH3 or CH2OH; R9, R11, R12, R13, R14, R15 represent CH3;

wherein R1, R2, R5, R8, R17, R18 represent OH; R3 represents OH, H orabsent; R9, R11, R12, R13, R14, R15 represent CH3.

A typical numbering 1 to 30 of carbon positions of a pentacyclictriterpene.

wherein R1, R2, R5, R8, R17, R18 represent OH; R9, R11, R12, R13, R14,R15 represent CH3, also named E4A or (E).

wherein R1, R2, R3, R4, R5, R8, R9, R10, R11, R12, R13, R14, R15, R16R17 represent H, OH, CH2OH, COOH, OR CH3, named as (P1), (P2).

This invention provides a method of synthesizing new active compounds. Amethod of attaching functional groups to the core compounds includingbut not limited to (A), (B), (C), (D1), (D2), (E), (F), (G), (H1), (H2),(J), E4A, E4A2Y, E6A, (P1), P(2),] involves esterification of corecompounds with acyl halide, wherein the halide including chloride,bromide, fluoride and iodide, wherein the acyl halide comprises acylchloride, wherein acyl chloride including but not limited to Tigloylchloride, angeloyl chloride, Acetyl chloride, Crotonoyl chloride,3,3-Dimethylartyloyl chloride, senecioyl chloride, Cinnamoyl chloride,Pentenoyl chloride, Hexanoyl chloride, benzoyl chloride, Ethylbutyrylchloride, Propionyl chloride, 2-Propenoyl chloride, Isobutyryl chloride,Butyryl chloride, (2E)-2-pentenoyl chloride, 4-Pentenoyl chloride,5-Hexenoyl chloride, Heptanoyl chloride, Octanoyl chloride, Nonanoylchloride, Decanoyl chloride, Lauroyl chloride, Myristoyl chloride,Oleoyl chloride for 5 sec, 1 min, 2 min, 5 min, 10 min, 30 min, 1 hr, 2hr, 18 hr, 2 days or 3 days at 0 C, 25 C or 75 C temperature. At the endof reaction, 5 ml of 2N HCl or 1M NaHCO3 is added to the reactionmixture. The solution is then extracted 3 times with 10 ml of ethylacetate which is then evaporated under vacuum and at 45 C andlyophilization. The reaction product is dissolved in 80%acetonitrile—0.005% Trifluoroacetic acid. The active esterificationproducts are purified with HPLC. MTT activity was performed to test theactivity of acyl chloride, solution after the reaction, individualfractions, and individual compounds. The core compounds are synthetic,semi synthetic or from natural source. The core compounds are includingterpene, isoprene, triterpenes, and hydroxylated triterpenes.

MTT activity of acylation of core compounds in different reaction timeperiod of (ASAP)5 sec, 1 min, 2 min, 5 min, 10 min, 30 min, 1 hr, 2 hr,18 hr, 2 days or 3 days at 0 C, 25 C or 75 C temperature were studied.HPLC profiles of esterification products of core compound E4A with acylhalide, wherein the halide comprise chloride, bromide, fluoride andiodide, wherein the acyl halide comprise acyl chloride, wherein acylchloride comprise tigloyl chloride, angeloyl chloride, acetyl chloride,crotonoyl chloride, 3,3-dimethylartyloyl chloride, senecioyl chloride,cinnamoyl chloride, pentenoyl chloride, hexanoyl chloride, benzoylchloride, ethylbutyryl chloride, propionyl chloride, 2-propenoylchloride, isobutyryl chloride, butyryl chloride, (2E)-2-pentenoylchloride, 4-Pentenoyl chloride, 5-hexenoyl chloride, heptanoyl chloride,octanoyl chloride, nonanoyl chloride, decanoyl chloride, Lauroylchloride, myristoyl chloride, oleoyl chloride show that the compoundsvary in composition when the time or temperature of the reaction ischanged. See example in FIGS. 1-12 (U.S. Ser. No. 14/313,080) andExperiments 1-29

The peaks, fractions and compounds are selected according to theactivities of times studies and the changes of peaks. Selecting the HPLCfractions for isolation is according to the cytotoxic activity of thereaction product obtained at a specific time. The compounds havingstrong to weak activities are selected and isolated. Selecting the HPLCfractions for isolation may be according to the cytotoxic activity oftimes studies and the change of peaks. The anti cancer activities arethe MTT studies of bone (U2OS), lung (H460), bladder (HTB-9), ovary(ES2), colon (HCT116), pancreas (Capan), ovary (OVCAR3), prostate(DU145), skin (SK-Mel-5), mouth (KB), kidney (A498), breast (MCF-7),liver (HepG2), brain (T98G), luekemia (K562), cervix (HeLa).

Esterification of core compound E4A with Tigloyl chloride and isolationof the compounds with HPLC give the following compounds: whereinTig=Tigloyl

R1 R2 R5 R8 R17 R18 Cytotoxicity activity E4A OH OH OH OH OH OH none A1OH OH OH OH O-Tig OH moderate A2 OH OH OH OH OH O-Tig moderate A3 OH OHOH OH O-Tig O-Tig strong A4 O-Tig OH OH OH O-Tig O-Tig moderate A5 OHO-Tig OH OH O-Tig O-Tig moderate A6 OH OH O-Tig OH O-Tig O-Tig moderateA7 OH OH OH O-Tig O-Tig O-Tig moderate A8 O-Tig O-Tig OH OH O-Tig O-Tigweak A9 OH O-Tig O-Tig OH O-Tig O-Tig weak A10 OH OH O-Tig O-Tig O-TigO-Tig weak A11 O-Tig OH O-Tig OH O-Tig O-Tig weak A12 OH O-Tig OH O-TigO-Tig O-Tig weak A13 O-Tig OH OH O-Tig O-Tig O-Tig weak A14 OH O-TigO-Tig OH O-Tig O-Tig weak A15 O-Tig O-Tig O-Tig OH O-Tig O-Tig weak A16O-Tig O-Tig OH O-Tig O-Tig O-Tig weak A17 O-Tig OH O-Tig O-Tig O-TigO-Tig weak A18 OH O-Tig O-Tig O-Tig O-Tig O-Tig weak A19 O-Tig O-TigO-Tig O-Tig O-Tig O-Tig none A20 O-Tig O-Tig OH OH OH O-Tig moderate A21O-Tig O-Tig OH OH O-Tig OH moderate A22 O-Tig O-Tig OH O-Tig OH OHmoderate A23 O-Tig O-Tig O-Tig OH OH OH moderate A24 O-Tig O-Tig OH OHOH OH moderate A25 O-Tig OH OH OH OH O-Tig moderate A26 OH O-Tig OH OHOH O-Tig moderate A27 OH OH O-Tig OH OH O-Tig moderate A28 OH OH OHO-Tig OH O-Tig moderate A29 O-Tig OH OH OH O-Tig OH moderate A30 OHO-Tig OH OH O-Tig OH moderate A31 OH OH O-Tig OH O-Tig OH moderate A32OH OH OH O-Tig O-Tig OH moderate

Esterification of core compound E4A with Angeloyl chloride and isolationof the compounds with HPLC give the following compounds: whereinAng=Angeloyl

R1 R2 R5 R8 R17 R18 Cytotoxicity activity E4A OH OH OH OH OH OH none G1OH OH OH OH O-Ang OH moderate G2 OH OH OH OH OH O-Ang moderate G3 OH OHOH OH O-Ang O-Ang strong G4 O-Ang OH OH OH O-Ang O-Ang moderate G5 OHO-Ang OH OH O-Ang O-Ang moderate G6 OH OH O-Ang OH O-Ang O-Ang moderateG7 OH OH OH O-Ang O-Ang O-Ang moderate G8 O-Ang O-Ang OH OH O-Ang O-Angweak G9 OH O-Ang O-Ang OH O-Ang O-Ang weak G10 OH OH O-Ang O-Ang O-AngO-Ang weak G11 O-Ang OH O-Ang OH O-Ang O-Ang weak G12 OH O-Ang OH O-AngO-Ang O-Ang weak G13 O-Ang OH OH O-Ang O-Ang O-Ang weak G14 OH O-AngO-Ang OH O-Ang O-Ang weak G15 O-Ang O-Ang O-Ang OH O-Ang O-Ang weak G16O-Ang O-Ang OH O-Ang O-Ang O-Ang weak G17 O-Ang OH O-Ang O-Ang O-AngO-Ang weak G18 OH O-Ang O-Ang O-Ang O-Ang O-Ang weak G19 O-Ang O-AngO-Ang O-Ang O-Ang O-Ang none G20 O-Ang O-Ang OH OH OH O-Ang moderate G21O-Ang O-Ang OH OH O-Ang OH moderate G22 O-Ang O-Ang OH O-Ang OH OHmoderate G23 O-Ang O-Ang O-Ang OH OH OH moderate G24 O-Ang O-Ang OH OHOH OH moderate G25 O-Ang OH OH OH OH O-Ang moderate G26 OH O-Ang OH OHOH O-Ang moderate G27 OH OH O-Ang OH OH O-Ang moderate G28 OH OH OHO-Ang OH O-Ang moderate G29 O-Ang OH OH OH O-Ang OH moderate G30 OHO-Ang OH OH O-Ang OH moderate G31 OH OH O-Ang OH O-Ang OH moderate G32OH OH OH O-Ang O-Ang OH moderate

Esterification of core compound E4A with (3,3-Dimethylartyloyl chloride)senecioyl chloride and isolation of the compounds with HPLC give thefollowing compounds: Wherein Sen=senecioyl

R1 R2 R5 R8 R17 R18 Cytotoxicity activity E4A OH OH OH OH OH OH none B1OH OH OH OH O-Sen OH moderate B2 OH OH OH OH OH O-Sen moderate B3 OH OHOH OH O-Sen O-Sen strong B4 O-Sen OH OH OH O-Sen O-Sen moderate B5 OHO-Sen OH OH O-Sen O-Sen moderate B6 OH OH O-Sen OH O-Sen O-Sen moderateB7 OH OH OH O-Sen O-Sen O-Sen moderate B8 O-Sen O-Sen OH OH O-Sen O-Senweak B9 OH O-Sen O-Sen OH O-Sen O-Sen weak B10 OH OH O-Sen O-Sen O-SenO-Sen weak B11 O-Sen OH O-Sen OH O-Sen O-Sen weak B12 OH O-Sen OH O-SenO-Sen O-Sen weak B13 O-Sen OH OH O-Sen O-Sen O-Sen weak B14 OH O-SenO-Sen OH O-Sen O-Sen weak B15 O-Sen O-Sen O-Sen OH O-Sen O-Sen weak B16O-Sen O-Sen OH O-Sen O-Sen O-Sen weak B17 O-Sen OH O-Sen O-Sen O-SenO-Sen weak B18 OH O-Sen O-Sen O-Sen O-Sen O-Sen weak B19 O-Sen O-SenO-Sen O-Sen O-Sen O-Sen none B20 O-Sen O-Sen OH OH OH O-Sen moderate B21O-Sen O-Sen OH OH O-Sen OH moderate B22 O-Sen O-Sen OH O-Sen OH OHmoderate B23 O-Sen O-Sen O-Sen OH OH OH moderate B24 O-Sen O-Sen OH OHOH OH moderate B25 O-Sen OH OH OH OH O-Sen moderate B26 OH O-Sen OH OHOH O-Sen moderate B27 OH OH O-Sen OH OH O-Sen moderate B28 OH OH OHO-Sen OH O-Sen moderate B29 O-Sen OH OH OH O-Sen OH moderate B30 OHO-Sen OH OH O-Sen OH moderate B31 OH OH O-Sen OH O-Sen OH moderate B32OH OH OH O-Sen O-Sen OH moderate

Esterification of core compound E4A with 4-Pentenoyl chloride andisolation of the compounds with HPLC give the following compounds:wherein Pen=4-Pentenoyl

R1 R2 R5 R8 R17 R18 Cytotoxicity activity E4A OH OH OH OH OH OH none C1OH OH OH OH O—Pen OH moderate C2 OH OH OH OH OH O—Pen moderate C3 OH OHOH OH O—Pen O—Pen strong C4 O—Pen OH OH OH O—Pen O—Pen moderate C5 OHO—Pen OH OH O—Pen O—Pen moderate C6 OH OH O—Pen OH O—Pen O—Pen moderateC7 OH OH OH O—Pen O—Pen O—Pen moderate C8 O—Pen O—Pen OH OH O—Pen O—Penweak C9 OH O—Pen O—Pen OH O—Pen O—Pen weak C10 OH OH O—Pen O—Pen O—PenO—Pen weak C11 O—Pen OH O—Pen OH O—Pen O—Pen weak C12 OH O—Pen OH O—PenO—Pen O—Pen weak C13 O—Pen OH OH O—Pen O—Pen O—Pen weak C14 OH O—PenO—Pen OH O—Pen O—Pen weak C15 O—Pen O—Pen O—Pen OH O—Pen O—Pen weak C16O—Pen O—Pen OH O—Pen O—Pen O—Pen weak C17 O—Pen OH O—Pen O—Pen O—PenO—Pen weak C18 OH O—Pen O—Pen O—Pen O—Pen O—Pen weak C19 O—Pen O—PenO—Pen O—Pen O—Pen O—Pen none C20 O—Pen O—Pen OH OH OH O—Pen moderate C21O—Pen O—Pen OH OH O—Pen OH moderate C22 O—Pen O—Pen OH O—Pen OH OHmoderate C23 O—Pen O—Pen O—Pen OH OH OH moderate C24 O—Pen O—Pen OH OHOH OH moderate C25 O—Pen OH OH OH OH O—Pen moderate C26 OH O—Pen OH OHOH O—Pen moderate C27 OH OH O—Pen OH OH O—Pen moderate C28 OH OH OHO—Pen OH O—Pen moderate C29 O—Pen OH OH OH O—Pen OH moderate C30 OHO—Pen OH OH O—Pen OH moderate C31 OH OH O—Pen OH O—Pen OH moderate C32OH OH OH O—Pen O—Pen OH moderate

Esterification of core compound E4A with Hexanoyl chloride and isolationof the compounds with HPLC give the following compounds: whereinHex=Hexanoyl

Cyto- toxicity R1 R2 R5 R8 R17 R18 activity E4A OH OH OH OH OH OH noneD1 OH OH OH OH O-Hex OH moderate D2 OH OH OH OH OH O-Hex moderate D3 OHOH OH OH O-Hex O-Hex strong D4 O-Hex OH OH OH O-Hex O-Hex moderate D5 OHO-Hex OH OH O-Hex O-Hex moderate D6 OH OH O-Hex OH O-Hex O-Hex moderateD7 OH OH OH O-Hex O-Hex O-Hex moderate D8 O-Hex O-Hex OH OH O-Hex O-Hexweak D9 OH O-Hex O-Hex OH O-Hex O-Hex weak D10 OH OH O-Hex O-Hex O-HexO-Hex weak D11 O-Hex OH O-Hex OH O-Hex O-Hex weak D12 OH O-Hex OH O-HexO-Hex O-Hex weak D13 O-Hex OH OH O-Hex O-Hex O-Hex weak D14 OH O-HexO-Hex OH O-Hex O-Hex weak D15 O-Hex O-Hex O-Hex OH O-Hex O-Hex weak D16O-Hex O-Hex OH O-Hex O-Hex O-Hex weak D17 O-Hex OH O-Hex O-Hex O-HexO-Hex weak D18 OH O-Hex O-Hex O-Hex O-Hex O-Hex weak D19 O-Hex O-HexO-Hex O-Hex O-Hex O-Hex none D20 O-Hex O-Hex OH OH OH O-Hex moderate D21O-Hex O-Hex OH OH O-Hex OH moderate D22 O-Hex O-Hex OH O-Hex OH OHmoderate D23 O-Hex O-Hex O-Hex OH OH OH moderate D24 O-Hex O-Hex OH OHOH OH moderate D25 O-Hex OH OH OH OH O-Hex moderate D26 OH O-Hex OH OHOH O-Hex moderate D27 OH OH O-Hex OH OH O-Hex moderate D28 OH OH OHO-Hex OH O-Hex moderate D29 O-Hex OH OH OH O-Hex OH moderate D30 OHO-Hex OH OH O-Hex OH moderate D31 OH OH O-Hex OH O-Hex OH moderate D32OH OH OH O-Hex O-Hex OH moderate

Esterification of core compound E4A with 2-Ethylbutyryl chloride andisolation of the compounds with HPLC give the following compounds:wherein Eth=2-Ethylbutyryl

Cyto- toxicity R1 R2 R5 R8 R17 R18 activity E4A OH OH OH OH OH OH noneE1 OH OH OH OH O-Eth OH moderate E2 OH OH OH OH OH O-Eth moderate E3 OHOH OH OH O-Eth O-Eth strong E4 O-Eth OH OH OH O-Eth O-Eth moderate E5 OHO-Eth OH OH O-Eth O-Eth moderate E6 OH OH O-Eth OH O-Eth O-Eth moderateE7 OH OH OH O-Eth O-Eth O-Eth moderate E8 O-Eth O-Eth OH OH O-Eth O-Ethweak E9 OH O-Eth O-Eth OH O-Eth O-Eth weak E10 OH OH O-Eth O-Eth O-EthO-Eth weak E11 O-Eth OH O-Eth OH O-Eth O-Eth weak E12 OH O-Eth OH O-EthO-Eth O-Eth weak E13 O-Eth OH OH O-Eth O-Eth O-Eth weak E14 OH O-EthO-Eth OH O-Eth O-Eth weak E15 O-Eth O-Eth O-Eth OH O-Eth O-Eth weak E16O-Eth O-Eth OH O-Eth O-Eth O-Eth weak E17 O-Eth OH O-Eth O-Eth O-EthO-Eth weak E18 OH O-Eth O-Eth O-Eth O-Eth O-Eth weak E19 O-Eth O-EthO-Eth O-Eth O-Eth O-Eth none E20 O-Eth O-Eth OH OH OH O-Eth moderate E21O-Eth O-Eth OH OH O-Eth OH moderate E22 O-Eth O-Eth OH O-Eth OH OHmoderate E23 O-Eth O-Eth O-Eth OH OH OH moderate E24 O-Eth O-Eth OH OHOH OH moderate E25 O-Eth OH OH OH OH O-Eth moderate E26 OH O-Eth OH OHOH O-Eth moderate E27 OH OH O-Eth OH OH O-Eth moderate E28 OH OH OHO-Eth OH O-Eth moderate E29 O-Eth OH OH OH O-Eth OH moderate E30 OHO-Eth OH OH O-Eth OH moderate E31 OH OH O-Eth OH O-Eth OH moderate E32OH OH OH O-Eth O-Eth OH moderate

Esterification of core compound E4A with Acetyl chloride (H) andisolation of the compounds with HPLC give the following compounds:wherein Acy=Acetyl

Cyto- toxicity R1 R2 R5 R8 R17 R18 activity E4A OH OH OH OH OH OH noneH1 OH OH OH OH O-Acy OH moderate H2 OH OH OH OH OH O-Acy moderate H3 OHOH OH OH O-Acy O-Acy strong H4 O-Acy OH OH OH O-Acy O-Acy moderate H5 OHO-Acy OH OH O-Acy O-Acy moderate H6 OH OH O-Acy OH O-Acy O-Acy moderateH7 OH OH OH O-Acy O-Acy O-Acy moderate H8 O-Acy O-Acy OH OH O-Acy O-Acyweak H9 OH O-Acy O-Acy OH O-Acy O-Acy weak H10 OH OH O-Acy O-Acy O-AcyO-Acy weak H11 O-Acy OH O-Acy OH O-Acy O-Acy weak H12 OH O-Acy OH O-AcyO-Acy O-Acy weak H13 O-Acy OH OH O-Acy O-Acy O-Acy weak H14 OH O-AcyO-Acy OH O-Acy O-Acy weak H15 O-Acy O-Acy O-Acy OH O-Acy O-Acy weak H16O-Acy O-Acy OH O-Acy O-Acy O-Acy weak H17 O-Acy OH O-Acy O-Acy O-AcyO-Acy weak H18 OH O-Acy O-Acy O-Acy O-Acy O-Acy weak H19 O-Acy O-AcyO-Acy O-Acy O-Acy O-Acy none H20 O-Acy O-Acy OH OH OH O-Acy moderate H21O-Acy O-Acy OH OH O-Acy OH moderate H22 O-Acy O-Acy OH O-Acy OH OHmoderate H23 O-Acy O-Acy O-Acy OH OH OH moderate H24 O-Acy O-Acy OH OHOH OH moderate H25 O-Acy OH OH OH OH O-Acy moderate H26 OH O-Acy OH OHOH O-Acy moderate H27 OH OH O-Acy OH OH O-Acy moderate H28 OH OH OHO-Acy OH O-Acy moderate H29 O-Acy OH OH OH O-Acy OH moderate H30 OHO-Acy OH OH O-Acy OH moderate H31 OH OH O-Acy OH O-Acy OH moderate H32OH OH OH O-Acy O-Acy OH moderate

Esterification of core compound E4A with Crotonoyl chloride andisolation of the compounds with HPLC give the following compounds:wherein Cro=Crotonoyl

Cyto- toxicity R1 R2 R5 R8 R17 R18 activity E4A OH OH OH OH OH OH none I1 OH OH OH OH O-Cro OH moderate I 2 OH OH OH OH OH O-Cro moderate I 3 OHOH OH OH O-Cro O-Cro strong I 4 O-Cro OH OH OH O-Cro O-Cro moderate I 5OH O-Cro OH OH O-Cro O-Cro moderate I 6 OH OH O-Cro OH O-Cro O-Cromoderate I 7 OH OH OH O-Cro O-Cro O-Cro moderate I 8 O-Cro O-Cro OH OHO-Cro O-Cro weak I 9 OH O-Cro O-Cro OH O-Cro O-Cro weak I 10 OH OH O-CroO-Cro O-Cro O-Cro weak I 11 O-Cro OH O-Cro OH O-Cro O-Cro weak I 12 OHO-Cro OH O-Cro O-Cro O-Cro weak I 13 O-Cro OH OH O-Cro O-Cro O-Cro weakI 14 OH O-Cro O-Cro OH O-Cro O-Cro weak I 15 O-Cro O-Cro O-Cro OH O-CroO-Cro weak I 16 O-Cro O-Cro OH O-Cro O-Cro O-Cro weak I 17 O-Cro OHO-Cro O-Cro O-Cro O-Cro weak I 18 OH O-Cro O-Cro O-Cro O-Cro O-Cro weakI 19 O-Cro O-Cro O-Cro O-Cro O-Cro O-Cro none I 20 O-Cro O-Cro OH OH OHO-Cro moderate I 21 O-Cro O-Cro OH OH O-Cro OH moderate I 22 O-Cro O-CroOH O-Cro OH OH moderate I 23 O-Cro O-Cro O-Cro OH OH OH moderate I 24O-Cro O-Cro OH OH OH OH moderate I 25 O-Cro OH OH OH OH O-Cro moderate I26 OH O-Cro OH OH OH O-Cro moderate I 27 OH OH O-Cro OH OH O-Cromoderate I 28 OH OH OH O-Cro OH O-Cro moderate I 29 O-Cro OH OH OH O-CroOH moderate I 30 OH O-Cro OH OH O-Cro OH moderate I 31 OH OH O-Cro OHO-Cro OH moderate I 32 OH OH OH O-Cro O-Cro OH moderate

Esterification of core compound E4A with Cinnamoyl chloride andisolation of the compounds with HPLC give the following compounds:wherein Cin=Cinnamoyl

Cyto- toxicity R1 R2 R5 R8 R17 R18 activity E4A OH OH OH OH OH OH noneJ1 OH OH OH OH O-Cin OH moderate J2 OH OH OH OH OH O-Cin moderate J3 OHOH OH OH O-Cin O-Cin strong J4 O-Cin OH OH OH O-Cin O-Cin moderate J5 OHO-Cin OH OH O-Cin O-Cin moderate J6 OH OH O-Cin OH O-Cin O-Cin moderateJ7 OH OH OH O-Cin O-Cin O-Cin moderate J8 O-Cin O-Cin OH OH O-Cin O-Cinweak J9 OH O-Cin O-Cin OH O-Cin O-Cin weak J10 OH OH O-Cin O-Cin O-CinO-Cin weak J11 O-Cin OH O-Cin OH O-Cin O-Cin weak J12 OH O-Cin OH O-CinO-Cin O-Cin weak J13 O-Cin OH OH O-Cin O-Cin O-Cin weak J14 OH O-CinO-Cin OH O-Cin O-Cin weak J15 O-Cin O-Cin O-Cin OH O-Cin O-Cin weak J16O-Cin O-Cin OH O-Cin O-Cin O-Cin weak J17 O-Cin OH O-Cin O-Cin O-CinO-Cin weak J18 OH O-Cin O-Cin O-Cin O-Cin O-Cin weak J19 O-Cin O-CinO-Cin O-Cin O-Cin O-Cin none J20 O-Cin O-Cin OH OH OH O-Cin moderate J21O-Cin O-Cin OH OH O-Cin OH moderate J22 O-Cin O-Cin OH O-Cin OH OHmoderate J23 O-Cin O-Cin O-Cin OH OH OH moderate J24 O-Cin O-Cin OH OHOH OH moderate J25 O-Cin OH OH OH OH O-Cin moderate J26 OH O-Cin OH OHOH O-Cin moderate J27 OH OH O-Cin OH OH O-Cin moderate J28 OH OH OHO-Cin OH O-Cin moderate J29 O-Cin OH OH OH O-Cin OH moderate J30 OHO-Cin OH OH O-Cin OH moderate J31 OH OH O-Cin OH O-Cin OH moderate J32OH OH OH O-Cin O-Cin OH moderate

Esterification of core compound E4A with benzoyl chloride and isolationof the compounds with HPLC give the following compounds: whereinBen=benzoyl

Cyto- toxicity R1 R2 R5 R8 R17 R18 activity E4A OH OH OH OH OH OH NoneK1 OH OH OH OH O-Ben OH moderate K2 OH OH OH OH OH O-Ben moderate K3 OHOH OH OH O-Ben O-Ben strong K4 O-Ben OH OH OH O-Ben O-Ben moderate K5 OHO-Ben OH OH O-Ben O-Ben moderate K6 OH OH O-Ben OH O-Ben O-Ben moderateK7 OH OH OH O-Ben O-Ben O-Ben moderate K8 O-Ben O-Ben OH OH O-Ben O-Benweak K9 OH O-Ben O-Ben OH O-Ben O-Ben weak K10 OH OH O-Ben O-Ben O-BenO-Ben weak K11 O-Ben OH O-Ben OH O-Ben O-Ben weak K12 OH O-Ben OH O-BenO-Ben O-Ben weak K13 O-Ben OH OH O-Ben O-Ben O-Ben weak K14 OH O-BenO-Ben OH O-Ben O-Ben weak K15 O-Ben O-Ben O-Ben OH O-Ben O-Ben weak K16O-Ben O-Ben OH O-Ben O-Ben O-Ben weak K17 O-Ben OH O-Ben O-Ben O-BenO-Ben weak K18 OH O-Ben O-Ben O-Ben O-Ben O-Ben weak K19 O-Ben O-BenO-Ben O-Ben O-Ben O-Ben none K20 O-Ben O-Ben OH OH OH O-Ben moderate K21O-Ben O-Ben OH OH O-Ben OH moderate K22 O-Ben O-Ben OH O-Ben OH OHmoderate K23 O-Ben O-Ben O-Ben OH OH OH moderate K24 O-Ben O-Ben OH OHOH OH moderate K25 O-Ben OH OH OH OH O-Ben moderate K26 OH O-Ben OH OHOH O-Ben moderate K27 OH OH O-Ben OH OH O-Ben moderate K28 OH OH OHO-Ben OH O-Ben moderate K29 O-Ben OH OH OH O-Ben OH moderate K30 OHO-Ben OH OH O-Ben OH moderate K31 OH OH O-Ben OH O-Ben OH moderate K32OH OH OH O-Ben O-Ben OH moderate

Esterification of core compound E4A with Propionyl chloride andisolation of the compounds with HPLC give the following compounds:wherein Ppi=Propionyl

Cyto- toxicity R1 R2 R5 R8 R17 R18 activity E4A OH OH OH OH OH OH noneK1 OH OH OH OH O-Ppi OH moderate K2 OH OH OH OH OH O-Ppi moderate K3 OHOH OH OH O-Ppi O-Ppi strong K4 O-Ppi OH OH OH O-Ppi O-Ppi moderate K5 OHO-Ppi OH OH O-Ppi O-Ppi moderate K6 OH OH O-Ppi OH O-Ppi O-Ppi moderateK7 OH OH OH O-Ppi O-Ppi O-Ppi moderate K8 O-Ppi O-Ppi OH OH O-Ppi O-Ppiweak K9 OH O-Ppi O-Ppi OH O-Ppi O-Ppi weak K10 OH OH O-Ppi O-Ppi O-PpiO-Ppi weak K11 O-Ppi OH O-Ppi OH O-Ppi O-Ppi weak K12 OH O-Ppi OH O-PpiO-Ppi O-Ppi weak K13 O-Ppi OH OH O-Ppi O-Ppi O-Ppi weak K14 OH O-PpiO-Ppi OH O-Ppi O-Ppi weak K15 O-Ppi O-Ppi O-Ppi OH O-Ppi O-Ppi weak K16O-Ppi O-Ppi OH O-Ppi O-Ppi O-Ppi weak K17 O-Ppi OH O-Ppi O-Ppi O-PpiO-Ppi weak K18 OH O-Ppi O-Ppi O-Ppi O-Ppi O-Ppi weak K19 O-Ppi O-PpiO-Ppi O-Ppi O-Ppi O-Ppi none K20 O-Ppi O-Ppi OH OH OH O-Ppi moderate K21O-Ppi O-Ppi OH OH O-Ppi OH moderate K22 O-Ppi O-Ppi OH O-Ppi OH OHmoderate K23 O-Ppi O-Ppi O-Ppi OH OH OH moderate K24 O-Ppi O-Ppi OH OHOH OH moderate K25 O-Ppi OH OH OH OH O-Ppi moderate K26 OH O-Ppi OH OHOH O-Ppi moderate K27 OH OH O-Ppi OH OH O-Ppi moderate K28 OH OH OHO-Ppi OH O-Ppi moderate K29 O-Ppi OH OH OH O-Ppi OH moderate K30 OHO-Ppi OH OH O-Ppi OH moderate K31 OH OH O-Ppi OH O-Ppi OH moderate K32OH OH OH O-Ppi O-Ppi OH moderate

Esterification of core compound E4A with 2-propenoyl chloride andisolation of the compounds with HPLC give the following compounds:wherein Ppe=Propenoyl

Cyto- toxicity R1 R2 R5 R8 R17 R18 activity E4A OH OH OH OH OH OH noneK1 OH OH OH OH O-Ppe OH moderate K2 OH OH OH OH OH O-Ppe moderate K3 OHOH OH OH O-Ppe O-Ppe strong K4 O-Ppe OH OH OH O-Ppe O-Ppe moderate K5 OHO-Ppe OH OH O-Ppe O-Ppe moderate K6 OH OH O-Ppe OH O-Ppe O-Ppe moderateK7 OH OH OH O-Ppe O-Ppe O-Ppe moderate K8 O-Ppe O-Ppe OH OH O-Ppe O-Ppeweak K9 OH O-Ppe O-Ppe OH O-Ppe O-Ppe weak K10 OH OH O-Ppe O-Ppe O-PpeO-Ppe weak K11 O-Ppe OH O-Ppe OH O-Ppe O-Ppe weak K12 OH O-Ppe OH O-PpeO-Ppe O-Ppe weak K13 O-Ppe OH OH O-Ppe O-Ppe O-Ppe weak K14 OH O-PpeO-Ppe OH O-Ppe O-Ppe weak K15 O-Ppe O-Ppe O-Ppe OH O-Ppe O-Ppe weak K16O-Ppe O-Ppe OH O-Ppe O-Ppe O-Ppe weak K17 O-Ppe OH O-Ppe O-Ppe O-PpeO-Ppe weak K18 OH O-Ppe O-Ppe O-Ppe O-Ppe O-Ppe weak K19 O-Ppe O-PpeO-Ppe O-Ppe O-Ppe O-Ppe none K20 O-Ppe O-Ppe OH OH OH O-Ppe moderate K21O-Ppe O-Ppe OH OH O-Ppe OH moderate K22 O-Ppe O-Ppe OH O-Ppe OH OHmoderate K23 O-Ppe O-Ppe O-Ppe OH OH OH moderate K24 O-Ppe O-Ppe OH OHOH OH moderate K25 O-Ppe OH OH OH OH O-Ppe moderate K26 OH O-Ppe OH OHOH O-Ppe moderate K27 OH OH O-Ppe OH OH O-Ppe moderate K28 OH OH OHO-Ppe OH O-Ppe moderate K29 O-Ppe OH OH OH O-Ppe OH moderate K30 OHO-Ppe OH OH O-Ppe OH moderate K31 OH OH O-Ppe OH O-Ppe OH moderate K32OH OH OH O-Ppe O-Ppe OH moderate

Esterification of core compound E4A with Isobutyryl chloride andisolation of the compounds with HPLC give the following compounds:wherein Iso=Isobutyryl

Cyto- toxicity R1 R2 R5 R8 R17 R18 activity E4A OH OH OH OH OH OH noneK1 OH OH OH OH O-Iso OH moderate K2 OH OH OH OH OH O-Iso moderate K3 OHOH OH OH O-Iso O-Iso strong K4 O-Iso OH OH OH O-Iso O-Iso moderate K5 OHO-Iso OH OH O-Iso O-Iso moderate K6 OH OH O-Iso OH O-Iso O-Iso moderateK7 OH OH OH O-Iso O-Iso O-Iso moderate K8 O-Iso O-Iso OH OH O-Iso O-Isoweak K9 OH O-Iso O-Iso OH O-Iso O-Iso weak K10 OH OH O-Iso O-Iso O-IsoO-Iso weak K11 O-Iso OH O-Iso OH O-Iso O-Iso weak K12 OH O-Iso OH O-IsoO-Iso O-Iso weak K13 O-Iso OH OH O-Iso O-Iso O-Iso weak K14 OH O-IsoO-Iso OH O-Iso O-Iso weak K15 O-Iso O-Iso O-Iso OH O-Iso O-Iso weak K16O-Iso O-Iso OH O-Iso O-Iso O-Iso weak K17 O-Iso OH O-Iso O-Iso O-IsoO-Iso weak K18 OH O-Iso O-Iso O-Iso O-Iso O-Iso weak K19 O-Iso O-IsoO-Iso O-Iso O-Iso O-Iso none K20 O-Iso O-Iso OH OH OH O-Iso moderate K21O-Iso O-Iso OH OH O-Iso OH moderate K22 O-Iso O-Iso OH O-Iso OH OHmoderate K23 O-Iso O-Iso O-Iso OH OH OH moderate K24 O-Iso O-Iso OH OHOH OH moderate K25 O-Iso OH OH OH OH O-Iso moderate K26 OH O-Iso OH OHOH O-Iso moderate K27 OH OH O-Iso OH OH O-Iso moderate K28 OH OH OHO-Iso OH O-Iso moderate K29 O-Iso OH OH OH O-Iso OH moderate K30 OHO-Iso OH OH O-Iso OH moderate K31 OH OH O-Iso OH O-Iso OH moderate K32OH OH OH O-Iso O-Iso OH moderate

Esterification of core compound E4A with Butyryl chloride and isolationof the compounds with HPLC give the following compounds: whereinBut=Butyryl

Cyto- toxicity R1 R2 R5 R8 R17 R18 activity E4A OH OH OH OH OH OH noneK1 OH OH OH OH O-But OH moderate K2 OH OH OH OH OH O-But moderate K3 OHOH OH OH O-But O-But strong K4 O-But OH OH OH O-But O-But moderate K5 OHO-But OH OH O-But O-But moderate K6 OH OH O-But OH O-But O-But moderateK7 OH OH OH O-But O-But O-But moderate K8 O-But O-But OH OH O-But O-Butweak K9 OH O-But O-But OH O-But O-But weak K10 OH OH O-But O-But O-ButO-But weak K11 O-But OH O-But OH O-But O-But weak K12 OH O-But OH O-ButO-But O-But weak K13 O-But OH OH O-But O-But O-But weak K14 OH O-ButO-But OH O-But O-But weak K15 O-But O-But O-But OH O-But O-But weak K16O-But O-But OH O-But O-But O-But weak K17 O-But OH O-But O-But O-ButO-But weak K18 OH O-But O-But O-But O-But O-But weak K19 O-But O-ButO-But O-But O-But O-But none K20 O-But O-But OH OH OH O-But moderate K21O-But O-But OH OH O-But OH moderate K22 O-But O-But OH O-But OH OHmoderate K23 O-But O-But O-But OH OH OH moderate K24 O-But O-But OH OHOH OH moderate K25 O-But OH OH OH OH O-But moderate K26 OH O-But OH OHOH O-But moderate K27 OH OH O-But OH OH O-But moderate K28 OH OH OHO-But OH O-But moderate K29 O-But OH OH OH O-But OH moderate K30 OHO-But OH OH O-But OH moderate K31 OH OH O-But OH O-But OH moderate K32OH OH OH O-But O-But OH moderate

Esterification of core compound E4A with (2E)-2-pentenoyl chloride andisolation of the compounds with HPLC give the following compounds:wherein 2pe=2-pentenoyl

Cyto- toxicity R1 R2 R5 R8 R17 R18 activity E4A OH OH OH OH OH OH noneK1 OH OH OH OH O-2pe OH moderate K2 OH OH OH OH OH O-2pe moderate K3 OHOH OH OH O-2pe O-2pe strong K4 O-2pe OH OH OH O-2pe O-2pe moderate K5 OHO-2pe OH OH O-2pe O-2pe moderate K6 OH OH O-2pe OH O-2pe O-2pe moderateK7 OH OH OH O-2pe O-2pe O-2pe moderate K8 O-2pe O-2pe OH OH O-2pe O-2peweak K9 OH O-2pe O-2pe OH O-2pe O-2pe weak K10 OH OH O-2pe O-2pe O-2peO-2pe weak K11 O-2pe OH O-2pe OH O-2pe O-2pe weak K12 OH O-2pe OH O-2peO-2pe O-2pe weak K13 O-2pe OH OH O-2pe O-2pe O-2pe weak K14 OH O-2peO-2pe OH O-2pe O-2pe weak K15 O-2pe O-2pe O-2pe OH O-2pe O-2pe weak K16O-2pe O-2pe OH O-2pe O-2pe O-2pe weak K17 O-2pe OH O-2pe O-2pe O-2peO-2pe weak K18 OH O-2pe O-2pe O-2pe O-2pe O-2pe weak K19 O-2pe O-2peO-2pe O-2pe O-2pe O-2pe none K20 O-2pe O-2pe OH OH OH O-2pe moderate K21O-2pe O-2pe OH OH O-2pe OH moderate K22 O-2pe O-2pe OH O-2pe OH OHmoderate K23 O-2pe O-2pe O-2pe OH OH OH moderate K24 O-2pe O-2pe OH OHOH OH moderate K25 O-2pe OH OH OH OH O-2pe moderate K26 OH O-2pe OH OHOH O-2pe moderate K27 OH OH O-2pe OH OH O-2pe moderate K28 OH OH OHO-2pe OH O-2pe moderate K29 O-2pe OH OH OH O-2pe OH moderate K30 OHO-2pe OH OH O-2pe OH moderate K31 OH OH O-2pe OH O-2pe OH moderate K32OH OH OH O-2pe O-2pe OH moderate

Esterification of core compound E4A with Octanoyl chloride and isolationof the compounds with HPLC give the following compounds: whereinOct=Octanoyl

Cyto- toxicity R1 R2 R5 R8 R17 R18 activity E4A OH OH OH OH OH OH noneK1 OH OH OH OH O-Oct OH moderate K2 OH OH OH OH OH O-Oct moderate K3 OHOH OH OH O-Oct O-Oct strong K4 O-Oct OH OH OH O-Oct O-Oct moderate K5 OHO-Oct OH OH O-Oct O-Oct moderate K6 OH OH O-Oct OH O-Oct O-Oct moderateK7 OH OH OH O-Oct O-Oct O-Oct moderate K8 O-Oct O-Oct OH OH O-Oct O-Octweak K9 OH O-Oct O-Oct OH O-Oct O-Oct weak K10 OH OH O-Oct O-Oct O-OctO-Oct weak K11 O-Oct OH O-Oct OH O-Oct O-Oct weak K12 OH O-Oct OH O-OctO-Oct O-Oct weak K13 O-Oct OH OH O-Oct O-Oct O-Oct weak K14 OH O-OctO-Oct OH O-Oct O-Oct weak K15 O-Oct O-Oct O-Oct OH O-Oct O-Oct weak K16O-Oct O-Oct OH O-Oct O-Oct O-Oct weak K17 O-Oct OH O-Oct O-Oct O-OctO-Oct weak K18 OH O-Oct O-Oct O-Oct O-Oct O-Oct weak K19 O-Oct O-OctO-Oct O-Oct O-Oct O-Oct none K20 O-Oct O-Oct OH OH OH O-Oct moderate K21O-Oct O-Oct OH OH O-Oct OH moderate K22 O-Oct O-Oct OH O-Oct OH OHmoderate K23 O-Oct O-Oct O-Oct OH OH OH moderate K24 O-Oct O-Oct OH OHOH OH moderate K25 O-Oct OH OH OH OH O-Oct moderate K26 OH O-Oct OH OHOH O-Oct moderate K27 OH OH O-Oct OH OH O-Oct moderate K28 OH OH OHO-Oct OH O-Oct moderate K29 O-Oct OH OH OH O-Oct OH moderate K30 OHO-Oct OH OH O-Oct OH moderate K31 OH OH O-Oct OH O-Oct OH moderate K32OH OH OH O-Oct O-Oct OH moderate

Esterification of core compound E4A with Decanoyl chloride and isolationof the compounds with HPLC give the following compounds: whereinDec=Decanoyl

Cyto- toxicity R1 R2 R5 R8 R17 R18 activity E4A OH OH OH OH OH OH noneK1 OH OH OH OH O-Dec OH moderate K2 OH OH OH OH OH O-Dec moderate K3 OHOH OH OH O-Dec O-Dec strong K4 O-Dec OH OH OH O-Dec O-Dec moderate K5 OHO-Dec OH OH O-Dec O-Dec moderate K6 OH OH O-Dec OH O-Dec O-Dec moderateK7 OH OH OH O-Dec O-Dec O-Dec moderate K8 O-Dec O-Dec OH OH O-Dec O-Decweak K9 OH O-Dec O-Dec OH O-Dec O-Dec weak K10 OH OH O-Dec O-Dec O-DecO-Dec weak K11 O-Dec OH O-Dec OH O-Dec O-Dec weak K12 OH O-Dec OH O-DecO-Dec O-Dec weak K13 O-Dec OH OH O-Dec O-Dec O-Dec weak K14 OH O-DecO-Dec OH O-Dec O-Dec weak K15 O-Dec O-Dec O-Dec OH O-Dec O-Dec weak K16O-Dec O-Dec OH O-Dec O-Dec O-Dec weak K17 O-Dec OH O-Dec O-Dec O-DecO-Dec weak K18 OH O-Dec O-Dec O-Dec O-Dec O-Dec weak K19 O-Dec O-DecO-Dec O-Dec O-Dec O-Dec none K20 O-Dec O-Dec OH OH OH O-Dec moderate K21O-Dec O-Dec OH OH O-Dec OH moderate K22 O-Dec O-Dec OH O-Dec OH OHmoderate K23 O-Dec O-Dec O-Dec OH OH OH moderate K24 O-Dec O-Dec OH OHOH OH moderate K25 O-Dec OH OH OH OH O-Dec moderate K26 OH O-Dec OH OHOH O-Dec moderate K27 OH OH O-Dec OH OH O-Dec moderate K28 OH OH OHO-Dec OH O-Dec moderate K29 O-Dec OH OH OH O-Dec OH moderate K30 OHO-Dec OH OH O-Dec OH moderate K31 OH OH O-Dec OH O-Dec OH moderate K32OH OH OH O-Dec O-Dec OH moderate

Esterification of core compound E4A with Myristoyl chloride andisolation of the compounds with HPLC give the following compounds:wherein Myr=Myristoyl

Cyto- toxicity R1 R2 R5 R8 R17 R18 activity E4A OH OH OH OH OH OH noneK1 OH OH OH OH O-Myr OH moderate K2 OH OH OH OH OH O-Myr moderate K3 OHOH OH OH O-Myr O-Myr strong K4 O-Myr OH OH OH O-Myr O-Myr moderate K5 OHO-Myr OH OH O-Myr O-Myr moderate K6 OH OH O-Myr OH O-Myr O-Myr moderateK7 OH OH OH O-Myr O-Myr O-Myr moderate K8 O-Myr O-Myr OH OH O-Myr O-Myrweak K9 OH O-Myr O-Myr OH O-Myr O-Myr weak K10 OH OH O-Myr O-Myr O-MyrO-Myr weak K11 O-Myr OH O-Myr OH O-Myr O-Myr weak K12 OH O-Myr OH O-MyrO-Myr O-Myr weak K13 O-Myr OH OH O-Myr O-Myr O-Myr weak K14 OH O-MyrO-Myr OH O-Myr O-Myr weak K15 O-Myr O-Myr O-Myr OH O-Myr O-Myr weak K16O-Myr O-Myr OH O-Myr O-Myr O-Myr weak K17 O-Myr OH O-Myr O-Myr O-MyrO-Myr weak K18 OH O-Myr O-Myr O-Myr O-Myr O-Myr weak K19 O-Myr O-MyrO-Myr O-Myr O-Myr O-Myr none K20 O-Myr O-Myr OH OH OH O-Myr moderate K21O-Myr O-Myr OH OH O-Myr OH moderate K22 O-Myr O-Myr OH O-Myr OH OHmoderate K23 O-Myr O-Myr O-Myr OH OH OH moderate K24 O-Myr O-Myr OH OHOH OH moderate K25 O-Myr OH OH OH OH O-Myr moderate K26 OH O-Myr OH OHOH O-Myr moderate K27 OH OH O-Myr OH OH O-Myr moderate K28 OH OH OHO-Myr OH O-Myr moderate K29 O-Myr OH OH OH O-Myr OH moderate K30 OHO-Myr OH OH O-Myr OH moderate K31 OH OH O-Myr OH O-Myr OH moderate K32OH OH OH O-Myr O-Myr OH moderate

Esterification of E4A-Tig-N with senecioyl chloride and isolation of thecompounds with HPLC give the following compounds:

Cyto- toxicity R1 R2 R5 R8 R17 R18 activity E4A-Tig-N OH OH OH OH O-TigOH moderate Tig-Sen-1 OH OH OH OH O-Tig O-Sen strong Tig-Sen-2 O-Sen OHOH OH O-Tig O-Sen moderate Tig-Sen-3 OH O-Sen OH OH O-Tig O-Sen moderateTig-Sen-4 OH OH O-Sen OH O-Tig O-Sen moderate Tig-Sen-5 O-Sen OH OH OHO-Tig OH moderate Tig-Sen-6 OH O-Sen OH OH O-Tig OH moderate

Esterification of E4A-Tig-N with Crotonoyl chloride and isolation of thecompounds with HPLC give the following compounds:

R1 R2 R5 R8 R17 R18 Cytotoxicity activity E4A-Tig-N OH OH OH OH O-Tig OHmoderate Tig-Cro-1 OH OH OH OH O-Tig O-Cro strong Tig-Cro-2 O-Cro OH OHOH O-Tig O-Cro moderate Tig-Cro-3 OH O-Cro OH OH O-Tig O-Cro moderateTig-Cro-4 OH OH O-Cro OH O-Tig O-Cro moderate Tig-Cro-5 O-Cro OH OH OHO-Tig OH moderate Tig-Cro-6 OH O-Cro OH OH O-Tig OH moderate

Esterification of E4A-Tig-N with Acetyl chloride and isolation of thecompounds with HPLC give the following compounds:

R1 R2 R5 R8 R17 R18 Cytotoxicity activity E4A-Tig-N OH OH OH OH O-Tig OHmoderate Tig-Acy-1 OH OH OH OH O-Tig O-Acy strong Tig-Acy-2 O-Acy OH OHOH O-Tig O-Acy moderate Tig-Acy-3 OH O-Acy OH OH O-Tig O-Acy moderateTig-Acy-4 OH OH O-Acy OH O-Tig O-Acy moderate Tig-Acy-5 O-Acy OH OH OHO-Tig OH moderate Tig-Acy-6 OH O-Acy OH OH O-Tig OH moderate

Esterification of E4A-Tig-N with 4-Pentenoyl chloride and isolation ofthe compounds with HPLC give the following compounds:

R1 R2 R5 R8 R17 R18 Cytotoxicity activity E4A-Tig-N OH OH OH OH O-Tig OHmoderate Tig-Pen-1 OH OH OH OH O-Tig O-Pen strong Tig-Pen-2 O-Pen OH OHOH O-Tig O-Pen moderate Tig-Pen-3 OH O-Pen OH OH O-Tig O-Pen moderateTig-Pen-4 OH OH O-Pen OH O-Tig O-Pen moderate Tig-Pen-5 O-Pen OH OH OHO-Tig OH moderate Tig-Pen-6 OH O-Pen OH OH O-Tig OH moderate

Esterification of E4A-Tig-N with Hexanoly chloride and isolation of thecompounds with HPLC give the following compounds:

R1 R2 R5 R8 R17 R18 Cytotoxicity activity E4A-Tig-N OH OH OH OH O-Tig OHmoderate Tig-Hex-1 OH OH OH OH O-Tig O-Hex strong Tig-Hex-2 O-Hex OH OHOH O-Tig O-Hex moderate Tig-Hex-3 OH O-Hex OH OH O-Tig O-Hex moderateTig-Hex-4 OH OH O-Hex OH O-Tig O-Hex moderate Tig-Hex-5 O-Hex OH OH OHO-Tig OH moderate Tig-Hex-6 OH O-Hex OH OH O-Tig OH moderate

Esterification of E4A-Tig-N with Cinnamoyl chloride and isolation of thecompounds with HPLC give the following compounds:

R1 R2 R5 R8 R17 R18 Cytotoxicity activity E4A-Tig-N OH OH OH OH O-Tig OHmoderate Tig-Cin-1 OH OH OH OH O-Tig O-Cin strong Tig-Cin-2 O-Cin OH OHOH O-Tig O-Cin moderate Tig-Cin-3 OH O-Cin OH OH O-Tig O-Cin moderateTig-Cin-4 OH OH O-Cin OH O-Tig O-Cin moderate Tig-Cin-5 O-Cin OH OH OHO-Tig OH moderate Tig-Cin-6 OH O-Cin OH OH O-Tig OH moderate

Esterification of E4A-Tig-N with Angeloyl chloride and isolation of thecompounds with HPLC give the following compounds:

R1 R2 R5 R8 R17 R18 Cytotoxicity activity E4A-Tig-N OH OH OH OH O-Tig OHmoderate Tig-Ang-1 OH OH OH OH O-Tig O-Ang strong Tig-Ang-2 O-Ang OH OHOH O-Tig O-Ang moderate Tig-Ang-3 OH O-Ang OH OH O-Tig O-Ang moderateTig-Ang-4 OH OH O-Ang OH O-Tig O-Ang moderate Tig-Ang-5 O-Ang OH OH OHO-Tig OH moderate Tig-Ang-6 OH O-Ang OH OH O-Tig OH moderate

Esterification of E4A-Tig-N with 2-Ethylbutyryl chloride and isolationof the compounds with HPLC give the following compounds:

R1 R2 R5 R8 R17 R18 Cytotoxicity activity E4A-Tig-N OH OH OH OH O-Tig OHmoderate Tig-Eth-1 OH OH OH OH O-Tig O-Eth strong Tig-Eth-2 O-Eth OH OHOH O-Tig O-Eth moderate Tig-Eth-3 OH O-Eth OH OH O-Tig O-Eth moderateTig-Eth-4 OH OH O-Eth OH O-Tig O-Eth moderate Tig-Eth-5 O-Eth OH OH OHO-Tig OH moderate Tig-Eth-6 OH O-Eth OH OH O-Tig OH moderate

Esterification of E4A-Tig-R with senecioyl chloride and isolation of thecompounds with HPLC give the following compounds:

R1 R2 R5 R8 R17 R18 Cytotoxicity activity E4A-Tig-R OH OH OH OH O-TigO-Tig strong Tig-R-Sen-1 O-Sen O-Sen OH OH O-Tig O-Tig weak Tig-R-Sen-2O-Sen OH OH OH O-Tig O-Tig moderate Tig-R-Sen-3 OH O-Sen OH OH O-TigO-Tig moderate Tig-R-Sen-4 OH OH O-Sen OH O-Tig O-Tig moderateTig-R-Sen-5 O-Sen OH O-Sen OH O-Tig O-Tig weak Tig-R-Sen-6 OH O-SenO-Sen OH O-Tig O-Tig weak

Esterification of E4A-Tig-R with Crotonoyl chloride and isolation of thecompounds with HPLC give the following compounds:

R1 R2 R5 R8 R17 R18 Cytotoxicity activity E4A-Tig-R OH OH OH OH O-TigO-Tig strong Tig-R-Cro-1 O-Cro O-Cro OH OH O-Tig O-Tig weak Tig-R-Cro-2O-Cro OH OH OH O-Tig O-Tig moderate Tig-R-Cro-3 OH O-Cro OH OH O-TigO-Tig moderate Tig-R-Cro-4 OH OH O-Cro OH O-Tig O-Tig moderateTig-R-Cro-5 O-Cro OH O-Cro OH O-Tig O-Tig weak Tig-R-Cro-6 OH O-CroO-Cro OH O-Tig O-Tig weak

Esterification of E4A-Tig-R with Acetyl chloride and isolation of thecompounds with HPLC give the following compounds:

R1 R2 R5 R8 R17 R18 Cytotoxicity activity E4A-Tig-R OH OH OH OH O-TigO-Tig strong Tig-R-Acy-1 O-Acy O-Acy OH OH O-Tig O-Tig weak Tig-R-Acy-2O-Acy OH OH OH O-Tig O-Tig moderate Tig-R-Acy-3 OH O-Acy OH OH O-TigO-Tig moderate Tig-R-Acy-4 OH OH O-Acy OH O-Tig O-Tig moderateTig-R-Acy-5 O-Acy OH O-Acy OH O-Tig O-Tig weak Tig-R-Acy-6 OH O-AcyO-Acy OH O-Tig O-Tig weak

Esterification of E4A-Tig-R with 4-Pentenoyl chloride and isolation ofthe compounds with HPLC give the following compounds:

R1 R2 R5 R8 R17 R18 Cytotoxicity activity E4A-Tig-R OH OH OH OH O-TigO-Tig strong Tig-R-Pen-1 O-Pen O-Pen OH OH O-Tig O-Tig weak Tig-R-Pen-2O-Pen OH OH OH O-Tig O-Tig moderate Tig-R-Pen-3 OH O-Pen OH OH O-TigO-Tig moderate Tig-R-Pen-4 OH OH O-Pen OH O-Tig O-Tig moderateTig-R-Pen-5 O-Pen OH O-Pen OH O-Tig O-Tig weak Tig-R-Pen-6 OH O-PenO-Pen OH O-Tig OH weak

Esterification of E4A-Tig-R with Hexanoly chloride and isolation of thecompounds with HPLC give the following compounds:

R1 R2 R5 R8 R17 R18 Cytotoxicity activity E4A-Tig-R OH OH OH OH O-TigO-Tig strong Tig-R-Hex-1 O-Hex O-Hex OH OH O-Tig O-Tig weak Tig-R-Hex-2O-Hex OH OH OH O-Tig O-Tig moderate Tig-R-Hex-3 OH O-Hex OH OH O-TigO-Tig moderate Tig-R-Hex-4 OH OH O-Hex OH O-Tig O-Tig moderateTig-R-Hex-5 O-Hex OH O-Hex OH O-Tig O-Tig weak Tig-R-Hex-6 OH O-HexO-Hex OH O-Tig O-Tig weak

Esterification of E4A-Tig-R with Cinnamoyl chloride and isolation of thecompounds with HPLC give the following compounds:

R1 R2 R5 R8 R17 R18 Cytotoxicity activity E4A-Tig-R OH OH OH OH O-TigO-Tig strong Tig-R-Cin-1 O-Cin O-Cin OH OH O-Tig O-Tig weak Tig-R-Cin-2O-Cin OH OH OH O-Tig O-Tig moderate Tig-R-Cin-3 OH O-Cin OH OH O-TigO-Tig moderate Tig-R-Cin-4 OH OH O-Cin OH O-Tig O-Tig moderateTig-R-Cin-5 O-Cin OH O-Cin OH O-Tig O-Tig weak Tig-R-Cin-6 OH O-CinO-Cin OH O-Tig O-Tig weak

Esterification of E4A-Tig-R with Angeloyl chloride and isolation of thecompounds with HPLC give the following compounds:

R1 R2 R5 R8 R17 R18 Cytotoxicity activity E4A-Tig-R OH OH OH OH O-TigO-Tig strong Tig-R-Ang-1 O-Ang O-Ang OH OH O-Tig O-Tig weak Tig-R-Ang-2O-Ang OH OH OH O-Tig O-Tig moderate Tig-R-Ang-3 OH O-Ang OH OH O-TigO-Tig moderate Tig-R-Ang-4 OH OH O-Ang OH O-Tig O-Tig moderateTig-R-Ang-5 O-Ang OH O-Ang OH O-Tig O-Tig weak Tig-R-Ang-6 OH O-AngO-Ang OH O-Tig O-Tig weak

Esterification of E4A-Tig-R with 2-Ethylbutyryl chloride and isolationof the compounds with HPLC give the following compounds:

R1 R2 R5 R8 R17 R18 Cytotoxicity activity E4A-Tig-R OH OH OH OH O-TigO-Tig strong Tig-R-Eth-1 O-Eth O-Eth OH OH O-Tig O-Tig weak Tig-R-Eth-2O-Eth OH OH OH O-Tig O-Tig moderate Tig-R-Eth-3 OH O-Eth OH OH O-TigO-Tig moderate Tig-R-Eth-4 OH OH O-Eth OH O-Tig O-Tig moderateTig-R-Eth-5 O-Eth OH O-Eth OH O-Tig O-Tig weak Tig-R-Eth-6 OH O-EthO-Eth OH O-Tig O-Tig weak

Esterification of compound (A), (B), (C), (D1), (D2), (E), (F), (G),(H1), (H2), E4A, E4A2Y, (P1), P(2), terpene, isoprene, triterpenes,hydroxylated triterpenes, with acyl halide, wherein the halide comprisechloride, bromide, fluoride and iodide, wherein the acyl halide compriseacyl chloride, wherein acyl chloride comprise tigloyl chloride, angeloylchloride, acetyl chloride, crotonoyl chloride, 3,3-dimethylartyloylchloride, senecioyl chloride, cinnamoyl chloride, pentenoyl chloride,hexanoyl chloride, benzoyl chloride, ethylbutyryl chloride, propionylchloride, 2-propenoyl chloride, isobutyryl chloride, butyryl chloride,(2E)-2-pentenoyl chloride, 4-Pentenoyl chloride, 5-hexenoyl chloride,heptanoyl chloride, octanoyl chloride, nonanoyl chloride, decanoylchloride, Lauroyl chloride, myristoyl chloride, oleoyl chloride. Thecompounds vary in composition when the time or temperature of thereaction is changed. The peaks, fractions and compounds are selectedaccording to the activities of times studies and the changes of peaks.The compounds having strong to weak activities are selected andisolated. The anti cancer activities (Cytotoxic Assay) are the MTTstudies of bone (U2OS), lung (H460), bladder (HTB-9), ovary (ES2), colon(HCT116), pancreas (Capan), ovary (OVCAR3), prostate (DU145), skin(SK-Mel-5), mouth (KB), kidney (A498), breast (MCF-7), liver (HepG2),brain (T98G), luekemia (K562), cervix (HeLa). The active esterificationproducts are purified with HPLC. The reaction product of mixtures andindividual compounds are tested with MTT Cytotoxic Assay. Details ofmethod are in Experiment 3 of the present application. A secondesterification of compound can be selected from the above experimentresults to produce new active compounds. A partial esterificationcompound is selected from the above experiments to perform a second orrepeated with a third esterification with different acyl chloride inorder to produce new active compounds with the experiments in thepresent application.

A method is 1) Dissolving core compound or triterpenes core,hydroxylated triterpenes core, in pyridine; 2) Adding acyl halide oracyl chloride; 3, The mixture is stirred for length of time including 5sec, 10 sec, 20 sec, 30 sec, 40 sec, 1 min, 2 min, 5 min, 10 min, 30min, 1 hr, 2 hr, 18 hr, 2 days or 3 days at different temperature; 4) Atthe end of reaction, aqueous solution of acid or weak base, or water isadded to the reaction mixture; 5) The solution is then extracted ofethyl acetate and ethyl acetate is removed by evaporation andlyophilization; 6) Dissolving the reaction product in acetonitrile withTrifluoroacetic acid or DMSO; 7) Testing the reaction product ofmixtures and individual fractions with MTT cytotoxic assay; 8) Selectingthe HPLC fractions for isolation is according to the cytotoxic activityof the reaction product obtained at a specific reaction time; 10)Purifiing the active esterification products with HPLC; 11) Collectingthe products; 12) Testing the products; wherein the core compound isterpene, isoprene, or triterpene core or hydroxylated triterpenes core;wherein the core compound was dissolved in pyridine; wherein the acylchloride including Tigloyl chloride, angeloyl chloride, Acetyl chloride,Crotonoyl chloride, 3,3-Dimethylartyloyl chloride, senecioyl chloride,Cinnamoyl chloride, Pentenoyl chloride, Hexanoyl chloride, benzoylchloride, Ethylbutyryl chloride, Propionyl chloride, 2-Propenoylchloride, Isobutyryl chloride, Butyryl chloride, (2E)-2-pentenoylchloride, 4-Pentenoyl chloride, 5-Hexenoyl chloride, Heptanoyl chloride,Octanoyl chloride, Nonanoyl chloride, Decanoyl chloride, Lauroylchloride, Myristoyl chloride, and Oleoyl chloride; wherein the reactiontime for the mixture is stirred for 5 sec, 10 sec, 20 sec, 30 sec, 40sec, 1 min, 2 min, 5 min, 10 min, 30 min, 1 hr, 2 hr, 18 hr, 2 days or 3days; wherein the temperature is 0 C, 25 C, 50 C or 75 C temperature;wherein the acid including HCl or the base including NaHCO3 is added tothe reaction mixture; wherein the solution is then extracted 3 timeswith ethyl acetate and lyophilization; wherein the reaction product isdissolved in 80% acetonitrile—0.005% Trifluoroacetic acid or DMSO;wherein selecting the HPLC fractions for isolation is according to thecytotoxic activity of the reaction product obtained at a reaction timeof 5 sec, 10 sec, 20 sec, 30 sec, 40 sec, 1 min, 2 min, 5 min, 10 min,30 min, 1 hr, 2 hr, 18 hr, 2 days or 3 days. In an embodiment, thereaction time may be ove 3 days. In an embodiment, the experiment may beperformed under 0 C. In an embodiment, the experiment may be performedover 75 C.

wherein (R1, R2, or R18) and (R17, R5 or R16) are independently selectedfrom the group of O-angeloyl, O-tigloyl, O-senecioyl, O-acetyl,O-Crotonoyl, O-3,3-Dimethylartyloyl, O-Cinnamoyl, O-Pentenoyl,O-Hexanoyl, O-benzoyl, O-Ethylbutyryl, O-dibenzoyl, O-benzoyl,O-alkanoyl, O-alkenoyl, O-benzoyl alkyl substituted O-alkanoyl,O-alkanoyl substituted phenyl, O-alkenoyl substituted phenyl, O-aryl,O-acyl, O-heterocylic, O-heteroraryl, O-alkenylcarbonyl, O-ethanoyl,O-propanoyl, O-propenoyl, O-butanoyl, O-butenoyl, O-pentanoyl,O-hexenoyl, O-heptanoyl, O-heptenoyl, O-octanoyl, O-octenoyl,O-nonanoyl, O-nonenoyl, O-decanoyl, O-decenoyl, O-propionyl,O-2-propenoyl, O-2-butenoyl, O-Isobutyryl, O-2-methylpropanoyl,O-2-ethylbutyryl, O-ethylbutanoyl, O-2-ethylbutanoyl, O-butyryl,O-(E)-2,3-Dimethylacryloyl, O-(E)-2-Methylcrotonoyl,O-3-cis-Methyl-methacryloyl, O-3-Methyl-2-butenoyl, O-3-Methylcrotonoyl,O-4-Pentenoyl, O-(2E)-2-pentenoyl, O-Caproyl, O-5-Hexenoyl, O-Capryloyl,O-Lauroyl, O-Dodecanoyl, O-Myristoyl, O-Tetradecanoyl, O-Oleoyl; whereinwherein R1, R2, R3, R4, R5, R8, R9, R10, R11, R12, R13, R14, R15, R16,R17 are independently selected from the group of H, OH, CH2OH, COOH, ORCH3,

The anti-cancer activities of Tig-R compound: IC50 of bone (U2OS) is 4.5ug/ml, lung (H460) is 4.8 ug/ml, bladder (HTB-9) is 2.5 ug/ml, ovary(ES2) is 2.8 ug/ml, colon (HCT116) is 5.2 ug/ml, pancreas (Capan) 2.4ug/ml, ovary (OVCAR3) is 5.8, prostate (DU145) is 3.6 ug/ml, skin(SK-Mel-5) is 5.1 ug/ml, mouth (KB) is 3 ug/ml, kidney (A498) 10 is 3.5ug/ml, breast (MCF-7) is 4.5 ug/ml, liver (HepG2) is 6 ug/ml, brain(T98G) is 8 ug/ml), leukemia (K562) is 2 ug/ml, cervix (HeLa) is 5ug/ml.

The anti-cancer activities of Tig-V compound: IC50 of bone (U2OS) is 7ug/ml, lung (H460) is 6.8 ug/ml, bladder (HTB-9) is 4 ug/ml, ovary (ES2)is 2 ug/ml, colon (HCT116) is 8 ug/ml, pancreas (Capan) 5 ug/ml, ovary(OVCAR3) is 9, prostate (DU145) is 4 ug/ml, skin (SK-Mel-5) is 6 ug/ml,mouth (KB) is 4.5 ug/ml, kidney (A498) is 4.8 ug/ml, breast (MCF-7) is 9ug/ml, liver (HepG2) is 12 ug/ml, brain (T98G) is 14 ug/ml), leukemia(K562) is 4 ug/ml, cervix (HeLa) is 7 ug/ml.

The anti-cancer activities of Tig-N compound: IC50 of bone (U2OS) is 15ug/ml, lung (H460) is 13 ug/ml, bladder (HTB-9) is 7.5 ug/ml, ovary(ES2) is 9 ug/ml, colon (HCT116) is 15 ug/ml, pancreas (Capan) 8 ug/ml,ovary (OVCAR3) is 18, prostate (DU145) is 4.8 ug/ml, skin (SK-Mel-5) is15 ug/ml, mouth (KB) is 9 ug/ml, kidney (A498) is 11 ug/ml, breast(MCF-7) is 13 ug/ml, liver (HepG2) is 18 ug/ml, brain (T98G) is 19ug/ml), leukemia (K562) is 6 ug/ml, cervix (HeLa) is 15 ug/ml.

The anti-cancer activities of Tig-Q compound: IC50 of bone (U2OS) is 20ug/ml, lung (H460) is 18 ug/ml, bladder (HTB-9) is 10 ug/ml, ovary (ES2)is 12 ug/ml, colon (HCT116) is 22 ug/ml, pancreas (Capan) 9 ug/ml, ovary(OVCAR3) is 23, prostate (DU145) is 15 ug/ml, skin (SK-Mel-5) is 20ug/ml, mouth (KB) is 12 ug/ml, kidney (A498) is 13 ug/ml, breast (MCF-7)is 18 ug/ml, liver (HepG2) is 24 ug/ml, brain (T98G) is 29 ug/ml),leukemia (K562) is 6 ug/ml, cervix (HeLa) is 20 ug/ml.

The anti-cancer activities of Tig-T compound: IC50 of bone (U2OS) is 20ug/ml, lung (H460) is 21 ug/ml, bladder (HTB-9) is 12 ug/ml, ovary (ES2)is 14 ug/ml, colon (HCT116) is 23 ug/ml, pancreas (Capan) 10 ug/ml,ovary (OVCAR3) is 25, prostate (DU145) is 16 ug/ml, skin (SK-Mel-5) is22 ug/ml, mouth (KB) is 13 ug/ml, kidney (A498) is 15 ug/ml, breast(MCF-7) is 20 ug/ml, liver (HepG2) is 26 ug/ml, brain (T98G) is 26ug/ml), leukemia (K562) is 9 ug/ml, cervix (HeLa) is 18 ug/ml.

The anti-cancer activities of Tig-S compound: IC50 of bone (U2OS) is 5.2ug/ml, lung (H460) is 5.6 ug/ml, bladder (HTB-9) is 3.5 ug/ml, ovary(ES2) is 0.1 ug/ml, colon (HCT116) is 6.6 ug/ml, pancreas (Capan) 2.9ug/ml, ovary (OVCAR3) is 6.5, prostate (DU145) is 4.3 ug/ml, skin(SK-Mel-5) is 5.8 ug/ml, mouth (KB) is 4 ug/ml, kidney (A498) is 4.8ug/ml, breast (MCF-7) is 6.3 ug/ml, liver (HepG2) is 8.5 ug/ml, brain(T98G) is 9 ug/ml), leukemia (K562) is 4.3 ug/ml, cervix (HeLa) is 7ug/ml.

The anti-cancer activities of Tig-U compound: IC50 of bone (U2OS) is 23ug/ml, lung (H460) is 19 ug/ml, bladder (HTB-9) is 15 ug/ml, ovary (ES2)is 17 ug/ml, colon (HCT116) is 26 ug/ml, pancreas (Capan) 9 ug/ml, ovary(OVCAR3) is 27, prostate (DU145) is 15 ug/ml, skin (SK-Mel-5) is 24ug/ml, mouth (KB) is 16 ug/ml, kidney (A498) is 18 ug/ml, breast (MCF-7)is 25 ug/ml, liver (HepG2) is 23 ug/ml, brain (T98G) is 22 ug/ml),leukemia (K562) is 10 ug/ml, cervix (HeLa) is 17 ug/ml.

The IC50 of Tig-R in normal human fibroblast cells (W138) is about 10-15ug/ml. This IC50 value is 3 times higher than those in ovary ES2 (2.8ug/ml) and lung (H460) is 4.8 ug/ml.

Swiss3T3 cells are mouse normal fibroblast which were used in thisexperiment to compare with ES2 (human ovarian cancer) in Tig-Rcytotoxicity determination. The preliminary results indicate that theIC50 of Tig-R in SW3T3 cells is above 20 ug/ml while the correspondingIC50 in ES2 cells is about 2.8 ug/ml.

This invention provides compounds, methods, or uses of a compound forthe manufacture of a medicament, or uses of a compound for medicamentselected from formula (2A), for treating cancers, inhibition of cancergrowth, cancer invasion, cells invasion, cancer cell invasion; celladhesion, cell attachment, cell circulating; for treating mad cowdisease; treating prion diseases; for inhibiting viruses; for preventingcerebral aging; for improving memory; improving cerebral functions; forcuring enuresis, frequent micturition, urinary incontinence; dementia,Alzheimer's disease, autism, brain trauma, Parkinson's disease or otherdiseases caused by cerebral dysfunctions or neurodegeneration; fortreating arthritis, rheumatism, poor blood circulation,arteriosclerosis, Raynaud's syndrome, angina pectoris, cardiac disorder,coronary heart disease, headache, dizziness, kidney disorder;cerebrovascular diseases; inhibiting NF-kappa B activation; for treatingbrain edema, severe acute respiratory syndrome, respiratory viraldiseases, chronic venous insufficiency, hypertension, chronic venousdisease, oedema, inflammation, hemorrhoids, peripheral edema formation,varicose vein disease, flu, post traumatic edema and postoperativeswelling; for inhibiting blood clots, for inhibiting ethanol absorption;for lowering blood sugar; for regulating adrenocorticotropin andcorticosterone levels; for Anti-MS, anti-aneurysm, anti-asthmatic,anti-oedematous, anti-inflammatory, anti-bradykinic,anti-capillarihemorrhagic, anti-cephalagic, anti-cervicobrachialgic,anti-eclamptic, anti-edemic, anti-encaphalitic, anti-epiglottitic,anti-exudative, anti-flu, anti-fracture, anti-gingivitic,anti-hematomic, anti-herpetic, anti-histaminic, anti-hydrathritic,anti-meningitic, antioxidant, anti-periodontic, anti-phlebitic,anti-pleuritic, anti-raucedo, anti-rhinitic, anti-tonsilitic,anti-ulcer, anti-varicose, anti-vertiginous, cancerostatic,corticosterogenic, diuretic, fungicide, hemolytic, hyaluronidaseinhibitor, lymphagogue, natriuretic, pesticide, pituitary stimulant,thymolytic, vasoprotective, inhibiting leishmaniases, modulatingadhesion or angiogenesis of cells, anti-parasitic; for improving bloodcirculation; soothing stroke; preventing plaque formation and promotetheir dissipated; improve blood viscosity; reducing cardiovascular;reducing cerebrovascular; reducing thrombosis, arteriosclerosis,coronary heart disease, hypertension, diabetes, thrombocytopeniapurpura, hemoptysis, hematemesis; treating blood in the stool, uterinebleeding, traumatic bleeding, abdominal irritation, swelling,fluttering, Blood circulation, swelling, pain; treating bronchiectasis,tuberculosis and lung abscess caused by too hemoptysis; reducingbleeding; antitussive; reducing expectorant; reducing analgesic effect;dilate blood vessels; reducing blood pressure; treatment of cerebralarteriosclerosis; elevating blood lipids; reducing cholesterol;manufacturing an adjuvant composition for treatment, using compoundsselected from the following:

R1, R2, R3, R4, R5, R8, R9, R10, R11, R12, R13, R14, R15, R16 areindependently selected from the group of O, hydrogen, hydroxyl, methyl,O-angeloyl, O-tigloyl, O-senecioyl, O-acetyl, O-Crotonoyl,O-3,3-Dimethylartyloyl, O-Cinnamoyl, O-Pentenoyl, O-Hexanoyl, O-benzoyl,O-Ethylbutyryl, O-alkyl, O-dibenzoyl, O-benzoyl, O-alkanoyl, O-alkenoyl,O-benzoyl alkyl substituted O-alkanoyl, O-alkanoyl substituted phenyl,O-alkenoyl substituted phenyl, O-aryl, O-acyl, O-heterocylic,O-heteroraryl, O-alkenylcarbonyl, O-ethanoyl, O-propanoyl, O-propenoyl,O-butanoyl, O-butenoyl, O-pentanoyl, O-hexenoyl, O-heptanoyl,O-heptenoyl, O-octanoyl, O-octenoyl, O-nonanoyl, O-nonenoyl, O-decanoyl,O-decenoyl, O-propionyl, O-2-propenoyl, O-2-butenoyl, O-Isobutyryl,O-2-methylpropanoyl, O-2-ethylbutyryl, O-ethylbutanoyl,O-2-ethylbutanoyl, O-butyryl, O-(E)-2,3-Dimethylacryloyl,O-(E)-2-Methylcrotonoyl, O-3-cis-Methyl-methacryloyl,O-3-Methyl-2-butenoyl, O-3-Methylcrotonoyl, O-4-Pentenoyl,O-(2E)-2-pentenoyl, O-Caproyl, O-5-Hexenoyl, O-Capryloyl, O-Lauroyl,O-Dodecanoyl, O-Myristoyl, O-Tetradecanoyl, O-Oleoyl, O—C(2-18) Acyl,CH2O-angeloyl, CH2O-tigloyl, CH2O-senecioyl, CH2O-acetyl,CH2O-Crotonoyl, CH2O-3,3-Dimethylartyloyl, CH2O-Cinnamoyl,CH2O-Pentenoyl, CH2O-Hexanoyl, CH2O-benzoyl, CH2O-Ethylbutyryl, CH3,CH2OH, CH2O-alkyl, CH2O-dibenzoyl, CH2O-benzoyl, CH2O-alkanoyl,CH2O-alkenoyl, CH2O-benzoyl alkyl substituted O-alkanoyl, CH2O-alkanoylsubstituted phenyl, CH2O-alkenoyl substituted phenyl, CH2O-aryl,CH2O-acyl, CH2O-heterocylic, CH2O-heteroraryl, CH2O-alkenylcarbonyl,CH2O-ethanoyl, CH2O-propanoyl, CH2O-propenoyl, CH2O-butanoyl,CH2O-butenoyl, CH2O-pentanoyl, CH2O-hexenoyl, CH2O-heptanoyl,CH2O-heptenoyl, CH2O-octanoyl, CH2O-octenoyl, CH2O-nonanoyl,CH2O-nonenoyl, CH2O-decanoyl, CH2O-decenoyl, CH2O-propionyl,CH2O-2-propenoyl, CH2O-2-butenoyl, CH2O-Isobutyryl,CH2O-2-methylpropanoyl, CH2O-2-ethylbutyryl, CH2O-ethylbutanoyl,CH2O-2-ethylbutanoyl, CH2O-butyryl, CH2O-(E)-2,3-Dimethylacryloyl,CH2O-(E)-2-Methylcrotonoyl, CH2O-3-cis-Methyl-methacryloyl,CH2O-3-Methyl-2-butenoyl, CH2O-3-Methylcrotonoyl, CH2O-4-Pentenoyl,CH2O-(2E)-2-pentenoyl, CH2O-Caproyl, CH2O-5-Hexenoyl, CH2O-Capryloyl,CH2O-Lauroyl, CH2O-Dodecanoyl, CH2O-Myristoyl, CH2O-Tetradecanoyl,CH2O-Oleoyl, CH2O—C(2-18) Acyl, alkane, alkene and sugar moiety orderivatives thereof; or wherein the structure (2A) comprises at least 2groups selected from O-angeloyl, O-tigloyl, O-senecioyl, O-acetyl,O-Crotonoyl, O-3,3-Dimethylartyloyl, O-Cinnamoyl, O-Pentenoyl,O-Hexanoyl, O-benzoyl, O-Ethylbutyryl, O-alkyl, O-dibenzoyl, O-benzoyl,O-alkanoyl, O-alkenoyl, O-benzoyl alkyl substituted O-alkanoyl,O-alkanoyl substituted phenyl, O-alkenoyl substituted phenyl, O-aryl,O-acyl, O-heterocylic, O-heteroraryl, O-alkenylcarbonyl, O-ethanoyl,O-propanoyl, O-propenoyl, O-butanoyl, O-butenoyl, O-pentanoyl,O-hexenoyl, O-heptanoyl, O-heptenoyl, O-octanoyl, O-octenoyl,O-nonanoyl, O-nonenoyl, O-decanoyl, O-decenoyl, O-propionyl,O-2-propenoyl, O-2-butenoyl, O-Isobutyryl, O-2-methylpropanoyl,O-2-ethylbutyryl, O-ethylbutanoyl, O-2-ethylbutanoyl, O-butyryl,O-(E)-2,3-Dimethylacryloyl, O-(E)-2-Methylcrotonoyl,O-3-cis-Methyl-methacryloyl, O-3-Methyl-2-butenoyl, O-3-Methylcrotonoyl,O-4-Pentenoyl, O-(2E)-2-pentenoyl, O-Caproyl, O-5-Hexenoyl, O-Capryloyl,O-Lauroyl, O-Dodecanoyl, O-Myristoyl, O-Tetradecanoyl, O-Oleoyl,O—C(2-18) Acyl; or wherein R1 and R2 are selected from O-angeloyl,O-tigloyl, O-senecioyl, O-acetyl, O-Crotonoyl, O-3,3-Dimethylartyloyl,O-Cinnamoyl, O-Pentenoyl, O-Hexanoyl, O-benzoyl, O-Ethylbutyryl,O-alkyl, O-dibenzoyl, O-benzoyl, O-alkanoyl, O-alkenoyl, O-benzoyl alkylsubstituted O-alkanoyl, O-alkanoyl substituted phenyl, O-alkenoylsubstituted phenyl, O-aryl, O-acyl, O-heterocylic, O-heteroraryl,O-alkenylcarbonyl, O-ethanoyl, O-propanoyl, O-propenoyl, O-butanoyl,O-butenoyl, O-pentanoyl, O-hexenoyl, O-heptanoyl, O-heptenoyl,O-octanoyl, O-octenoyl, O-nonanoyl, O-nonenoyl, O-decanoyl, O-decenoyl,O-propionyl, O-2-propenoyl, O-2-butenoyl, O-Isobutyryl,O-2-methylpropanoyl, O-2-ethylbutyryl, O-ethylbutanoyl,O-2-ethylbutanoyl, O-butyryl, O-(E)-2,3-Dimethylacryloyl,O-(E)-2-Methylcrotonoyl, O-3-cis-Methyl-methacryloyl,O-3-Methyl-2-butenoyl, O-3-Methylcrotonoyl, O-4-Pentenoyl,O-(2E)-2-pentenoyl, O-Caproyl, O-5-Hexenoyl, O-Capryloyl, O-Lauroyl,O-Dodecanoyl, O-Myristoyl, O-Tetradecanoyl, O-Oleoyl, O—C(2-18) Acyl; orwherein R4 and R10 are selected from CH2O-angeloyl, CH2O-tigloyl,CH2O-senecioyl, CH2O-acetyl, CH2O-Crotonoyl, CH2O-3,3-Dimethylartyloyl,CH2O-Cinnamoyl, CH2O-Pentenoyl, CH2O-Hexanoyl, CH2O-benzoyl,CH2O-Ethylbutyryl, CH3, CH2OH, CH2O-alkyl, CH2O-dibenzoyl, CH2O-benzoyl,CH2O-alkanoyl, CH2O-alkenoyl, CH2O-benzoyl alkyl substituted O-alkanoyl,CH2O-alkanoyl substituted phenyl, CH2O-alkenoyl substituted phenyl,CH2O-aryl, CH2O-acyl, CH2O-heterocylic, CH2O-heteroraryl,CH2O-alkenylcarbonyl, CH2O-ethanoyl, CH2O-propanoyl, CH2O-propenoyl,CH2O-butanoyl, CH2O-butenoyl, CH2O-pentanoyl, CH2O-hexenoyl,CH2O-heptanoyl, CH2O-heptenoyl, CH2O-octanoyl, CH2O-octenoyl,CH2O-nonanoyl, CH2O-nonenoyl, CH2O-decanoyl, CH2O-decenoyl,CH2O-propionyl, CH2O-2-propenoyl, CH2O-2-butenoyl, CH2O-Isobutyryl,CH2O-2-methylpropanoyl, CH2O-2-ethylbutyryl, CH2O-ethylbutanoyl,CH2O-2-ethylbutanoyl, CH2O-butyryl, CH2O-(E)-2,3-Dimethylacryloyl,CH2O-(E)-2-Methylcrotonoyl, CH2O-3-cis-Methyl-methacryloyl,CH2O-3-Methyl-2-butenoyl, CH2O-3-Methylcrotonoyl, CH2O-4-Pentenoyl,CH2O-(2E)-2-pentenoyl, CH2O-Caproyl, CH2O-5-Hexenoyl, CH2O-Capryloyl,CH2O-Lauroyl, CH2O-Dodecanoyl, CH2O-Myristoyl, CH2O-Tetradecanoyl,CH2O-Oleoyl, CH2O—C(2-18) Acyl. In an embodiment, R1 and R2 are attachedOH. In an embodiment, R4 and R10 are attached a CH2O-angeloyl,CH2O-tigloyl, CH2O-senecioyl, CH2O-acetyl, CH2O-Crotonoyl,CH2O-3,3-Dimethylartyloyl, CH2O-Cinnamoyl, CH2O-Pentenoyl,CH2O-Hexanoyl, CH2O-benzoyl, or CH2O-Ethylbutyryl. In an embodiment, R3and R8 is hydrogen or hydroxyl, In an embodiment, R9, R11, R12, R13, R14and R15 are independently attached with a methyl. In an embodiment, R4represents CH3, CHO, CH₂R6 or COR6, wherein R6 is selected fromhydroxyl, O-angeloyl, O-tigloyl, O-senecioyl, O-acetyl, O-Crotonoyl,O-3,3-Dimethylartyloyl, O-Cinnamoyl, O-Pentenoyl, O-Hexanoyl,O-Ethylbutyryl, O-alkyl, O-dibenzoyl, O-benzoyl, O-alkanoyl, O-alkenoyl,O-benzoyl alkyl substituted O-alkanoyl, O-alkanoyl substituted phenyl,O-alkenoyl substituted phenyl, O-aryl, O-acyl, O-heterocylic,O-heteroraryl, O-alkenylcarbonyl, O-ethanoyl, O-propanoyl, O-propenoyl,O-butanoyl, O-butenoyl, O-pentanoyl, O-hexenoyl, O-heptanoyl,O-heptenoyl, O-octanoyl, O-octenoyl, O-nonanoyl, O-nonenoyl, O-decanoyl,O-decenoyl, O-propionyl, O-2-propenoyl, O-2-butenoyl, O-Isobutyryl,O-2-methylpropanoyl, O-2-ethylbutyryl, O-ethylbutanoyl,O-2-ethylbutanoyl, O-butyryl, O-(E)-2,3-Dimethylacryloyl,O-(E)-2-Methylcrotonoyl, O-3-cis-Methyl-methacryloyl,O-3-Methyl-2-butenoyl, O-3-Methylcrotonoyl, O-4-Pentenoyl,O-(2E)-2-pentenoyl, O-Caproyl, O-5-Hexenoyl, O-Capryloyl, O-Lauroyl,O-Dodecanoyl, O-Myristoyl, O-Tetradecanoyl, O-Oleoyl, O—C(2-18) Acyl andderivatives thereof. In an embodiment, R3 is H or OH; In an embodiment,wherein R8 is H or OH; In an embodiment, R16 is H, CH3, OH, or R4 andR16 may together form —CH2-X—, CH(OH)—X— or C(═O)—X—, wherein the —X—may be O or NH or S; wherein when the C12-13 of ring 3 of the triterpenehas a double bond then R16 is H or absent. In an embodiment, R10represents CH3, CHO, or CH₂R6, wherein R6 is selected from hydroxyl,O-angeloyl, O-tigloyl, O-senecioyl, O-acetyl, O-Crotonoyl,O-3,3-Dimethylartyloyl, O-Cinnamoyl, O-Pentenoyl, O-Hexanoyl, O-benzoyl,O-Ethylbutyryl, O-alkyl, O-dibenzoyl, O-benzoyl, O-alkanoyl, O-alkenoyl,O-benzoyl alkyl substituted O-alkanoyl, O-alkanoyl substituted phenyl,O-alkenoyl substituted phenyl, O-aryl, O-acyl, O-heterocylic,O-heteroraryl, O-alkenylcarbonyl, O-ethanoyl, O-propanoyl, O-propenoyl,O-butanoyl, O-butenoyl, O-pentanoyl, O-hexenoyl, O-heptanoyl,O-heptenoyl, O-octanoyl, O-octenoyl, O-nonanoyl, O-nonenoyl, O-decanoyl,O-decenoyl, O-propionyl, O-2-propenoyl, O-2-butenoyl, O-Isobutyryl,O-2-methylpropanoyl, O-2-ethylbutyryl, O-ethylbutanoyl,O-2-ethylbutanoyl, O-butyryl, O-(E)-2,3-Dimethylacryloyl,O-(E)-2-Methylcrotonoyl, O-3-cis-Methyl-methacryloyl,O-3-Methyl-2-butenoyl, O-3-Methylcrotonoyl, O-4-Pentenoyl,O-(2E)-2-pentenoyl, O-Caproyl, O-5-Hexenoyl, O-Capryloyl, O-Lauroyl,O-Dodecanoyl, O-Myristoyl, O-Tetradecanoyl, O-Oleoyl, O—C(2-18) Acyl andderivatives thereof; In an embodiment, R5 is a hydrogen, hydroxyl,heterocyclic or O-sugar moiety(ies), wherein the sugar moiety(ies)is/are selected from a group but not limited of glucose, galactose,rhamnose, arabinose, xylose, fucose, allose, altrose, gulose, idose,lyxose, mannose, psicose, ribose, sorbose, tagatose, talose, fructose,alduronic acid, glucuronic acid, galacturonic acid, and derivatives orcombinations thereof; In embodiment, the above structures were attachedan 0 with double bond; wherein R9, R10, R11, R12, R13, R14, R15, R16 areindependently attached a group selecting from CH₃, CH₂OH, CHO, COOH,COO-alkyl, COO-aryl, COO-heterocyclic, COO-heteroaryl, CH₂Oaryl, CH₂O—heterocyclic, CH₂O— heteroaryl, alkyls group, hydroxyl, acetyl group;wherein R4 and R16 form a divalent radical of formula CH2O, CH(OR7)O, orCOOR7, wherein R7 is hydrogen, alkyl, angeloyl, tigloyl, senecioyl,dibenzoyl, benzoyl, alkanoyl, alkenoyl, benzoyl alkyl substitutedalkanoyl, aryl, acyl, heterocylic, heteroraryl, and derivatives thereof;wherein at least two of R1, R2 and R6 are attached a group selected fromO-angeloyl, O-tigloyl, O-senecioyl, O-acetyl, O-Crotonoyl,O-3,3-Dimethylartyloyl, O-Cinnamoyl, O-Pentenoyl, O-Hexanoyl, O-benzoyl,O-Ethylbutyryl, O-dibenzoyl, O-benzoyl, O-alkanoyl, O-alkenoyl,O-benzoyl alkyl substituted O-alkanoyl, O-aryl, O-acyl, O-heterocylic,O-heteroraryl, O-ethanoyl, O-propanoyl, O-propenoyl, O-butanoyl,O-butenoyl, O-pentanoyl, O-hexenoyl, O-heptanoyl, O-heptenoyl,O-octanoyl, O-octenoyl, O-nonanoyl, O-nonenoyl, O-decanoyl, O-decenoyl,O-propionyl, O-2-propenoyl, O-2-butenoyl, O-Isobutyryl,O-2-methylpropanoyl, O-2-ethylbutyryl, O-ethylbutanoyl,O-2-ethylbutanoyl, O-butyryl, O-(E)-2,3-Dimethylacryloyl,O-(E)-2-Methylcrotonoyl, O-3-cis-Methyl-methacryloyl,O-3-Methyl-2-butenoyl, O-3-Methylcrotonoyl, O-4-Pentenoyl,O-(2E)-2-pentenoyl, O-Caproyl, O-5-Hexenoyl, O-Capryloyl, O-Lauroyl,O-Dodecanoyl, O-Myristoyl, O-Tetradecanoyl, O-Oleoyl, O—C(2-18) Acyl andderivatives thereof; or at least one of R1, R2, and R4 is a sugar moietyhaving at least two groups selected from a group consisting of angeloyl,acetyl, tigloyl, senecioyl, Crotonoyl, 3,3-Dimethylartyloyl, Cinnamoyl,Pentenoyl, Hexanoyl, benzoyl, Ethylbutyryl, benzoyl, dibenzoyl,alkanoyl, alkenoyl, benzoyl alkyl substituted alkanoyl, aryl, acyl,heterocylic, heteroraryl, ethanoyl, propanoyl, propenoyl, butanoyl,butenoyl, pentanoyl, hexenoyl, heptanoyl, heptenoyl, octanoyl, octenoyl,nonanoyl, nonenoyl, decanoyl, decenoyl, propionyl, 2-propenoyl,2-butenoyl, Isobutyryl, 2-methylpropanoyl, 2-ethylbutyryl,ethylbutanoyl, 2-ethylbutanoyl, butyryl, (E)-2,3-Dimethylacryloyl,(E)-2-Methylcrotonoyl, 3-cis-Methyl-methacryloyl, 3-Methyl-2-butenoyl,3-Methylcrotonoyl, 4-Pentenoyl, (2E)-2-pentenoyl, Caproyl, 5-Hexenoyl,Capryloyl, Lauroyl, Dodecanoyl, Myristoyl, Tetradecanoyl, Oleoyl,O—C(2-18) Acyl and their derivatives thereof; or wherein R4 representsCH₂R6, wherein R6 is selected from hydroxyl, O-angeloyl, O-tigloyl,O-senecioyl, O-acetyl, O-Crotonoyl, O-3,3-Dimethylartyloyl, O-Cinnamoyl,O-Pentenoyl, O-Hexanoyl, O-Ethylbutyryl, O-alkyl, O-dibenzoyl,O-benzoyl, O-alkanoyl, O-alkenoyl, O-benzoyl alkyl substitutedO-alkanoyl, O-alkanoyl substituted phenyl, O-alkenoyl substitutedphenyl, O-aryl, O-acyl, O-heterocylic, O-heteroraryl, O-alkenylcarbonyland derivatives thereof; wherein R5 is/are the sugar moiety(ies)selected from the following sugars and alduronic acids: glucose,galactose, rhamnose, arabinose, xylose, fucose, allose, altrose, gulose,idose, lyxose, mannose, psicose, ribose, sorbose, tagatose, talose,fructose, glucuronic acid, galacturonic acid; or their derivativesthereof, In an embodiment, R5 is a hydroxyl, O-angeloyl, O-tigloyl,O-senecioyl, O-acetyl, O-Crotonoyl, O-3,3-Dimethylartyloyl, O-Cinnamoyl,O-Pentenoyl, O-Hexanoyl, O-benzoyl, O-Ethylbutyryl, O-alkyl,O-dibenzoyl, O-benzoyl, O-alkanoyl, O-alkenoyl, O-benzoyl alkylsubstituted O-alkanoyl, O-alkanoyl substituted phenyl, O-alkenoylsubstituted phenyl, O-aryl, O-acyl, O-heterocylic, O-heteroraryl,O-alkenylcarbonyl and derivatives thereof. In an embodiment, R1, R2, R3,R4, R5, R8, R9, R10, R11, R12, R13, R14 or R15 comprise of one or moresugar moieties. In an embodiment, R1, R2, R3, R4, R5, R8, R9, R10, R11,R12, R13, R14 or R15 comprise of one or more acids. In an embodiment, atleast 1, or 2, or 3, or 4 of R1, R2, R3, R4, R5, R8, R9, R10, R11, R12,R13, R14 and R15 is hydroxyl. In an embodiment, at least 2, or 3, or 4,or 5, or 6, or 7 of R1, R2, R3, R4, R5, R8, R9, R10, R11, R12, R13, R14and R15 are independently attached a group selected from the group ofO-acetyl, O-angeloyl, O-tigloyl, O-senecioyl, O-acetyl, O-Crotonoyl,O-3,3-Dimethylartyloyl, O-Cinnamoyl, O-Pentenoyl, O-Hexanoyl, O-benzoyl,O-Ethylbutyryl, O-alkyl, O-dibenzoyl, O-benzoyl, O-alkanoyl, O-alkenoyl,O-benzoyl alkyl substituted O-alkanoyl, O-alkanoyl substituted phenyl,O-alkenoyl substituted phenyl, O-aryl, O-acyl, O-heterocylic,O-heteroraryl, O-alkenylcarbonyl, alkane, alkene and derivativesthereof, wherein the group is attached to the triterpene directly or byconnecting moiety(ies); In an embodiment, at least 1 or 2, or 3, or 4,or 5, or 6, or 7 of R1, R2, R3, R4, R5, R8 and R10 are independentlyattached a group selected from the group of O-angeloyl, O-tigloyl,O-senecioyl, O-acetyl, O-Crotonoyl, O-3,3-Dimethylartyloyl, O-Cinnamoyl,O-Pentenoyl, O-Hexanoyl, O-benzoyl, O-Ethylbutyryl, O-alkyl,O-dibenzoyl, O-benzoyl, O-alkanoyl, O-alkenoyl, O-benzoyl alkylsubstituted O-alkanoyl, O-alkanoyl substituted phenyl, O-alkenoylsubstituted phenyl, O-aryl, O-acyl, O-heterocylic, O-heteroraryl,O-alkenylcarbonyl, O-ethanoyl, O-propanoyl, O-propenoyl, O-butanoyl,O-butenoyl, O-pentanoyl, O-hexenoyl, O-heptanoyl, O-heptenoyl,O-octanoyl, O-octenoyl, O-nonanoyl, O-nonenoyl, O-decanoyl, O-decenoyl,O-propionyl, O-2-propenoyl, O-2-butenoyl, O-Isobutyryl,O-2-methylpropanoyl, O-2-ethylbutyryl, O-ethylbutanoyl,O-2-ethylbutanoyl, O-butyryl, O-(E)-2,3-Dimethylacryloyl,O-(E)-2-Methylcrotonoyl, O-3-cis-Methyl-methacryloyl,O-3-Methyl-2-butenoyl, O-3-Methylcrotonoyl, O-4-Pentenoyl,O-(2E)-2-pentenoyl, O-Caproyl, O-5-Hexenoyl, O-Capryloyl, O-Lauroyl,O-Dodecanoyl, O-Myristoyl, O-Tetradecanoyl, O-Oleoyl, O—C(2-18) Acyl,CH2O-angeloyl, CH2O-tigloyl, CH2O-senecioyl, CH2O-acetyl,CH2O-Crotonoyl, CH2O-3,3-Dimethylartyloyl, CH2O-Cinnamoyl,CH2O-Pentenoyl, CH2O-Hexanoyl, CH2O-benzoyl, CH2O-Ethylbutyryl, CH3,CH2OH, CH2O-alkyl, CH2O-dibenzoyl, CH2O-benzoyl, CH2O-alkanoyl,CH2O-alkenoyl, CH2O-benzoyl alkyl substituted O-alkanoyl, CH2O-alkanoylsubstituted phenyl, CH2O-alkenoyl substituted phenyl, CH2O-aryl,CH2O-acyl, CH2O-heterocylic, CH2O-heteroraryl, CH2O-alkenylcarbonyl,CH2O-ethanoyl, CH2O-propanoyl, CH2O-propenoyl, CH2O-butanoyl,CH2O-butenoyl, CH2O-pentanoyl, CH2O-hexenoyl, CH2O-heptanoyl,CH2O-heptenoyl, CH2O-octanoyl, CH2O-octenoyl, CH2O-nonanoyl,CH2O-nonenoyl, CH2O-decanoyl, CH2O-decenoyl, CH2O-propionyl,CH2O-2-propenoyl, CH2O-2-butenoyl, CH2O-Isobutyryl,CH2O-2-methylpropanoyl, CH2O-2-ethylbutyryl, CH2O-ethylbutanoyl,CH2O-2-ethylbutanoyl, CH2O-butyryl, CH2O-(E)-2,3-Dimethylacryloyl,CH2O-(E)-2-Methylcrotonoyl, CH2O-3-cis-Methyl-methacryloyl,CH2O-3-Methyl-2-butenoyl, CH2O-3-Methylcrotonoyl, CH2O-4-Pentenoyl,CH2O-(2E)-2-pentenoyl, CH2O-Caproyl, CH2O-5-Hexenoyl, CH2O-Capryloyl,CH2O-Lauroyl, CH2O-Dodecanoyl, CH2O-Myristoyl, CH2O-Tetradecanoyl,CH2O-Oleoyl, CH2O—C(2-18) Acyl and derivatives thereof, wherein thegroup is attached to the triterpene directly or by connectingmoiety(ies). In embodiment, the compound is attached a sugarmoiety(ies), acid moiety(ies) or alduronic acid.

In an embodiment, the cancers comprise breast cancer, leukocytic cancer,liver cancer, ovarian cancer, bladder cancer, prostatic cancer, skincancer, bone cancer, brain cancer, leukemia cancer, lung cancer, coloncancer, CNS cancer, melanoma cancer, renal cancer, cervical cancer,esophageal cancer, testicular cancer, spleenic cancer, kidney cancer,lymphhatic cancer, pancreatic cancer, stomach cancer and thyroid cancer;wherein the cells comprise breast cell, leukocytic cell, liver cell,ovarian cell, bladder cell, prostatic cell, skin cell, bone cell, braincell, leukemia cell, lung cell, colon cell, CNS cell, melanoma cell,renal cell, cervical cell, esophageal cell, testicular cell, spleeniccell, kidney cell, lymphhatic cell, pancreatic cell, stomach cell andthyroid cell. In an embodiment, the compound is selected from thestructure:

R1, R2, R3, R4, R5, R8, R9, R10, R11, R12, R13, R14, R15 areindependently selected from the group of CH3, CH2OH, COOH, O, hydrogen,hydroxyl, methyl, O-angeloyl, O-tigloyl, O-senecioyl, O-acetyl,O-Crotonoyl, O-3,3-Dimethylartyloyl, O-Cinnamoyl, O-Pentenoyl,O-Hexanoyl, O-benzoyl, O-Ethylbutyryl, O-alkyl, O-dibenzoyl, O-benzoyl,O-alkanoyl, O-alkenoyl, O-benzoyl alkyl substituted O-alkanoyl,O-alkanoyl substituted phenyl, O-alkenoyl substituted phenyl, O-aryl,O-acyl, O-heterocylic, O-heteroraryl, O-alkenylcarbonyl, O-alkane,O-alkene, O-sugar moiety, O-ethanoyl, O-propanoyl, O-propenoyl,O-butanoyl, O-butenoyl, O-pentanoyl, O-hexenoyl, O-heptanoyl,O-heptenoyl, O-octanoyl, O-octenoyl, O-nonanoyl, O-nonenoyl, O-decanoyl,O-decenoyl, O-propionyl, O-2-propenoyl, O-2-butenoyl, O-Isobutyryl,O-2-methylpropanoyl, O-2-ethylbutyryl, O-ethylbutanoyl,O-2-ethylbutanoyl, O-butyryl, O-(E)-2,3-Dimethylacryloyl,O-(E)-2-Methylcrotonoyl, O-3-cis-Methyl-methacryloyl,O-3-Methyl-2-butenoyl, O-3-Methylcrotonoyl, O-4-Pentenoyl,O-(2E)-2-pentenoyl, O-Caproyl, O-5-Hexenoyl, O-Capryloyl, O-Lauroyl,O-Dodecanoyl, O-Myristoyl, O-Tetradecanoyl, O-Oleoyl, O—C(2-18) Acyl,CH2O-angeloyl, CH2O-tigloyl, CH2O-senecioyl, CH2O-acetyl,CH2O-Crotonoyl, CH2O-3,3-Dimethylartyloyl, CH2O-Cinnamoyl,CH2O-Pentenoyl, CH2O-Hexanoyl, CH2O-benzoyl, CH2O-Ethylbutyryl,CH2O-alkyl, CH2O-dibenzoyl, CH2O-benzoyl, CH2O-alkanoyl, CH2O-alkenoyl,CH2O-benzoyl alkyl substituted O-alkanoyl, CH2O-alkanoyl substitutedphenyl, CH2O-alkenoyl substituted phenyl, CH2O-aryl, CH2O-acyl,CH2O-heterocylic, CH2O-heteroraryl, CH2O-alkenylcarbonyl, CH2O-alkane,CH2O-alkene and CH2O-sugar moiety, CH2O-angeloyl, CH2O-tigloyl,CH2O-senecioyl, CH2O-acetyl, CH2O-Crotonoyl, CH2O-3,3-Dimethylartyloyl,CH2O-Cinnamoyl, CH2O-Pentenoyl, CH2O-Hexanoyl, CH2O-benzoyl,CH2O-Ethylbutyryl, CH3, CH2OH, CH2O-alkyl, CH2O-dibenzoyl, CH2O-benzoyl,CH2O-alkanoyl, CH2O-alkenoyl, CH2O-benzoyl alkyl substituted O-alkanoyl,CH2O-alkanoyl substituted phenyl, CH2O-alkenoyl substituted phenyl,CH2O-aryl, CH2O-acyl, CH2O-heterocylic, CH2O-heteroraryl,CH2O-alkenylcarbonyl, CH2O-ethanoyl, CH2O-propanoyl, CH2O-propenoyl,CH2O-butanoyl, CH2O-butenoyl, CH2O-pentanoyl, CH2O-hexenoyl,CH2O-heptanoyl, CH2O-heptenoyl, CH2O-octanoyl, CH2O-octenoyl,CH2O-nonanoyl, CH2O-nonenoyl, CH2O-decanoyl, CH2O-decenoyl,CH2O-propionyl, CH2O-2-propenoyl, CH2O-2-butenoyl, CH2O-Isobutyryl,CH2O-2-methylpropanoyl, CH2O-2-ethylbutyryl, CH2O-ethylbutanoyl,CH2O-2-ethylbutanoyl, CH2O-butyryl, CH2O-(E)-2,3-Dimethylacryloyl,CH2O-(E)-2-Methylcrotonoyl, CH2O-3-cis-Methyl-methacryloyl,CH2O-3-Methyl-2-butenoyl, CH2O-3-Methylcrotonoyl, CH2O-4-Pentenoyl,CH2O-(2E)-2-pentenoyl, CH2O-Caproyl, CH2O-5-Hexenoyl, CH2O-Capryloyl,CH2O-Lauroyl, CH2O-Dodecanoyl, CH2O-Myristoyl, CH2O-Tetradecanoyl,CH2O-Oleoyl, CH2O—C(2-18) Acyl, (CnH2n)O-angeloyl, (CnH2n)O-tigloyl,(CnH2n)O-senecioyl, (CnH2n)O-acetyl, (CnH2n)O-Crotonoyl,(CnH2n)O-3,3-Dimethylartyloyl, (CnH2n)O-Cinnamoyl, (CnH2n)O-Pentenoyl,(CnH2n)O-Hexanoyl, (CnH2n)O-benzoyl, (CnH2n)O-Ethylbutyryl,(CnH2n)O-alkyl, (CnH2n)O-dibenzoyl, (CnH2n)O-benzoyl, (CnH2n)O-alkanoyl,(CnH2n)O-alkenoyl, (CnH2n)O-benzoyl alkyl substituted O-alkanoyl,(CnH2n)O-alkanoyl substituted phenyl, (CnH2n)O-alkenoyl substitutedphenyl, (CnH2n)O-aryl, (CnH2n)O-acyl, (CnH2n)O-heterocylic,(CnH2n)O-heteroraryl, (CnH2n)O-alkenylcarbonyl, (CnH2n)O-alkane,(CnH2n)O-alkene and (CnH2n)O-sugar moiety, wherein n is 1 or 2 or 3 or 4or over 5 or derivatives thereof; or wherein any 1 or 2 or 3 or 4 of R1,R2, R3, R4, R5, R8 and R10 are independently attached an O-angeloyl,O-tigloyl, O-senecioyl, O-acetyl, O-Crotonoyl, O-3,3-Dimethylartyloyl,O-Cinnamoyl, O-Pentenoyl, O-Hexanoyl, O-benzoyl, O-Ethyl butyryl,O-alkyl, O-dibenzoyl, O-benzoyl, O-alkanoyl, O-alkenoyl, O-benzoyl alkylsubstituted O-alkanoyl, O-alkanoyl substituted phenyl, O-alkenoylsubstituted phenyl, O-aryl, O-acyl, O-heterocylic, O-heteroraryl,O-alkenylcarbonyl, O-ethanoyl, O-propanoyl, O-propenoyl, O-butanoyl,O-butenoyl, O-pentanoyl, O-hexenoyl, O-heptanoyl, O-heptenoyl,O-octanoyl, O-octenoyl, O-nonanoyl, O-nonenoyl, O-decanoyl, O-decenoyl,O-propionyl, O-2-propenoyl, O-2-butenoyl, O-Isobutyryl,O-2-methylpropanoyl, O-2-ethylbutyryl, O-ethylbutanoyl,O-2-ethylbutanoyl, O-butyryl, O-(E)-2,3-Dimethylacryloyl,O-(E)-2-Methylcrotonoyl, O-3-cis-Methyl-methacryloyl,O-3-Methyl-2-butenoyl, O-3-Methylcrotonoyl, O-4-Pentenoyl,O-(2E)-2-pentenoyl, O-Caproyl, O-5-Hexenoyl, O-Capryloyl, O-Lauroyl,O-Dodecanoyl, O-Myristoyl, O-Tetradecanoyl, O-Oleoyl, O—C(2-18) Acyl,CH2O-angeloyl, CH2O-tigloyl, CH2O-senecioyl, CH2O-acetyl,CH2O-Crotonoyl, CH2O-3,3-Dimethylartyloyl, CH2O-Cinnamoyl,CH2O-Pentenoyl, CH2O-Hexanoyl, CH2O-benzoyl, CH2O-Ethylbutyryl, CH3,CH2OH, CH2O-alkyl, CH2O-dibenzoyl, CH2O-benzoyl, CH2O-alkanoyl,CH2O-alkenoyl, CH2O-benzoyl alkyl substituted O-alkanoyl, CH2O-alkanoylsubstituted phenyl, CH2O-alkenoyl substituted phenyl, CH2O-aryl,CH2O-acyl, CH2O-heterocylic, CH2O-heteroraryl, CH2O-alkenylcarbonyl,CH2O-ethanoyl, CH2O-propanoyl, CH2O-propenoyl, CH2O-butanoyl,CH2O-butenoyl, CH2O-pentanoyl, CH2O-hexenoyl, CH2O-heptanoyl,CH2O-heptenoyl, CH2O-octanoyl, CH2O-octenoyl, CH2O-nonanoyl,CH2O-nonenoyl, CH2O-decanoyl, CH2O-decenoyl, CH2O-propionyl,CH2O-2-propenoyl, CH2O-2-butenoyl, CH2O-Isobutyryl,CH2O-2-methylpropanoyl, CH2O-2-ethylbutyryl, CH2O-ethylbutanoyl,CH2O-2-ethylbutanoyl, CH2O-butyryl, CH2O-(E)-2,3-Dimethylacryloyl,CH2O-(E)-2-Methylcrotonoyl, CH2O-3-cis-Methyl-methacryloyl,CH2O-3-Methyl-2-butenoyl, CH2O-3-Methylcrotonoyl, CH2O-4-Pentenoyl,CH2O-(2E)-2-pentenoyl, CH2O-Caproyl, CH2O-5-Hexenoyl, CH2O-Capryloyl,CH2O-Lauroyl, CH2O-Dodecanoyl, CH2O-Myristoyl, CH2O-Tetradecanoyl,CH2O-Oleoyl, CH2O—C(2-18) Acyl; or wherein R9, R11, R12, R13, R14, R15are independently attached a CH3; or wherein R10 is attached anO-angeloyl, O-tigloyl, O-senecioyl, O-acetyl, O-Crotonoyl,O-3,3-Dimethylartyloyl, O-Cinnamoyl, O-Pentenoyl, O-Hexanoyl, O-benzoyl,O-Ethylbutyryl, O-alkyl, O-dibenzoyl, O-benzoyl, O-alkanoyl, O-alkenoyl,O-benzoyl alkyl substituted O-alkanoyl, O-alkanoyl substituted phenyl,O-alkenoyl substituted phenyl, O-aryl, O-acyl, O-heterocylic,O-heteroraryl, O-alkenylcarbonyl, O-ethanoyl, O-propanoyl, O-propenoyl,O-butanoyl, O-butenoyl, O-pentanoyl, O-hexenoyl, O-heptanoyl,O-heptenoyl, O-octanoyl, O-octenoyl, O-nonanoyl, O-nonenoyl, O-decanoyl,O-decenoyl, O-propionyl, O-2-propenoyl, O-2-butenoyl, O-Isobutyryl,O-2-methylpropanoyl, O-2-ethylbutyryl, O-ethylbutanoyl,O-2-ethylbutanoyl, O-butyryl, O-(E)-2,3-Dimethylacryloyl,O-(E)-2-Methylcrotonoyl, O-3-cis-Methyl-methacryloyl,O-3-Methyl-2-butenoyl, O-3-Methylcrotonoyl, O-4-Pentenoyl,O-(2E)-2-pentenoyl, O-Caproyl, O-5-Hexenoyl, O-Capryloyl, O-Lauroyl,O-Dodecanoyl, O-Myristoyl, O-Tetradecanoyl, O-Oleoyl, O—C(2-18) Acyl,CH2O-angeloyl, CH2O-tigloyl, CH2O-senecioyl, CH2O-acetyl,CH2O-Crotonoyl, CH2O-3,3-Dimethylartyloyl, CH2O-Cinnamoyl,CH2O-Pentenoyl, CH2O-Hexanoyl, CH2O-benzoyl, CH2O-Ethylbutyryl, CH3,CH2OH, CH2O-alkyl, CH2O-dibenzoyl, CH2O-benzoyl, CH2O-alkanoyl,CH2O-alkenoyl, CH2O-benzoyl alkyl substituted O-alkanoyl, CH2O-alkanoylsubstituted phenyl, CH2O-alkenoyl substituted phenyl, CH2O-aryl,CH2O-acyl, CH2O-heterocylic, CH2O-heteroraryl, CH2O-alkenylcarbonyl,CH2O-ethanoyl, CH2O-propanoyl, CH2O-propenoyl, CH2O-butanoyl,CH2O-butenoyl, CH2O-pentanoyl, CH2O-hexenoyl, CH2O-heptanoyl,CH2O-heptenoyl, CH2O-octanoyl, CH2O-octenoyl, CH2O-nonanoyl,CH2O-nonenoyl, CH2O-decanoyl, CH2O-decenoyl, CH2O-propionyl,CH2O-2-propenoyl, CH2O-2-butenoyl, CH2O-Isobutyryl,CH2O-2-methylpropanoyl, CH2O-2-ethylbutyryl, CH2O-ethylbutanoyl,CH2O-2-ethylbutanoyl, CH2O-butyryl, CH2O-(E)-2,3-Dimethylacryloyl,CH2O-(E)-2-Methylcrotonoyl, CH2O-3-cis-Methyl-methacryloyl,CH2O-3-Methyl-2-butenoyl, CH2O-3-Methylcrotonoyl, CH2O-4-Pentenoyl,CH2O-(2E)-2-pentenoyl, CH2O-Caproyl, CH2O-5-Hexenoyl, CH2O-Capryloyl,CH2O-Lauroyl, CH2O-Dodecanoyl, CH2O-Myristoyl, CH2O-Tetradecanoyl,CH2O-Oleoyl, CH2O—C(2-18) Acyl; or wherein R4 and/or R10 areindependently attached an O-angeloyl, O-tigloyl, O-senecioyl, O-acetyl,O-Crotonoyl, O-3,3-Dimethylartyloyl, O-Cinnamoyl, O-Pentenoyl,O-Hexanoyl, O-benzoyl, O-Ethylbutyryl, O-alkyl, O-dibenzoyl, O-benzoyl,O-alkanoyl, O-alkenoyl, O-benzoyl alkyl substituted O-alkanoyl,O-alkanoyl substituted phenyl, O-alkenoyl substituted phenyl, O-aryl,O-acyl, O-heterocylic, O-heteroraryl, O-alkenylcarbonyl, O-ethanoyl,O-propanoyl, O-propenoyl, O-butanoyl, O-butenoyl, O-pentanoyl,O-hexenoyl, O-heptanoyl, O-heptenoyl, O-octanoyl, O-octenoyl,O-nonanoyl, O-nonenoyl, O-decanoyl, O-decenoyl, O-propionyl,O-2-propenoyl, O-2-butenoyl, O-Isobutyryl, O-2-methylpropanoyl,O-2-ethylbutyryl, O-ethylbutanoyl, O-2-ethylbutanoyl, O-butyryl,O-(E)-2,3-Dimethylacryloyl, O-(E)-2-Methylcrotonoyl,O-3-cis-Methyl-methacryloyl, O-3-Methyl-2-butenoyl, O-3-Methylcrotonoyl,O-4-Pentenoyl, O-(2E)-2-pentenoyl, O-Caproyl, O-5-Hexenoyl, O-Capryloyl,O-Lauroyl, O-Dodecanoyl, O-Myristoyl, O-Tetradecanoyl, O-Oleoyl,O—C(2-18) Acyl, CH2O-angeloyl, CH2O-tigloyl, CH2O-senecioyl,CH2O-acetyl, CH2O-Crotonoyl, CH2O-3,3-Dimethylartyloyl, CH2O-Cinnamoyl,CH2O-Pentenoyl, CH2O-Hexanoyl, CH2O-benzoyl, CH2O-Ethylbutyryl, CH3,CH2OH, CH2O-alkyl, CH2O-dibenzoyl, CH2O-benzoyl, CH2O-alkanoyl,CH2O-alkenoyl, CH2O-benzoyl alkyl substituted O-alkanoyl, CH2O-alkanoylsubstituted phenyl, CH2O-alkenoyl substituted phenyl, CH2O-aryl,CH2O-acyl, CH2O-heterocylic, CH2O-heteroraryl, CH2O-alkenylcarbonyl,CH2O-ethanoyl, CH2O-propanoyl, CH2O-propenoyl, CH2O-butanoyl,CH2O-butenoyl, CH2O-pentanoyl, CH2O-hexenoyl, CH2O-heptanoyl,CH2O-heptenoyl, CH2O-octanoyl, CH2O-octenoyl, CH2O-nonanoyl,CH2O-nonenoyl, CH2O-decanoyl, CH2O-decenoyl, CH2O-propionyl,CH2O-2-propenoyl, CH2O-2-butenoyl, CH2O-Isobutyryl,CH2O-2-methylpropanoyl, CH2O-2-ethylbutyryl, CH2O-ethylbutanoyl,CH2O-2-ethylbutanoyl, CH2O-butyryl, CH2O-(E)-2,3-Dimethylacryloyl,CH2O-(E)-2-Methylcrotonoyl, CH2O-3-cis-Methyl-methacryloyl,CH2O-3-Methyl-2-butenoyl, CH2O-3-Methylcrotonoyl, CH2O-4-Pentenoyl,CH2O-(2E)-2-pentenoyl, CH2O-Caproyl, CH2O-5-Hexenoyl, CH2O-Capryloyl,CH2O-Lauroyl, CH2O-Dodecanoyl, CH2O-Myristoyl, CH2O-Tetradecanoyl,CH2O-Oleoyl, CH2O—C(2-18) Acyl; wherein R3 is OH or H or absent; whereinR1, R2, R3, R5, R8 are OH or H or absent; wherein R9, R11, R12, R13,R14, and R15 are CH3; or wherein R1, R2, R5, R8 represent OH; R3represents OH, H or absent; or wherein R4, R10 represent CH2Oangeloyl;R9, R11, R12, R13, R14, R15 represent CH3; or wherein R1, R2, R5, R8represent OH or O-tigloyl; R3 represents OH, H, or absent; or whereinR4, R10 represent CH2O tigloyl; R9, R11, R12, R13, R14, R15 representCH3; wherein the group attaching to the core compound selected fromacetyl, angeloyl, tigloyl, senecioyl, Crotonoyl, 3,3-Dimethylartyloyl,Cinnamoyl, Pentenoyl, Hexanoyl, benzoyl, Ethylbutyryl, alkyl, dibenzoyl,benzoyl, methylbutanoyl, methylpropanoyl, alkanoyl, alkenoyl, benzoylalkyl substituted alkanoyl, alkanoyl substituted phenyl, alkenoylsubstituted phenyl, aryl, acyl, heterocylic, heteroraryl,alkenylcarbonyl, ethanoyl, propanoyl, propenoyl, butanoyl, butenoyl,pentanoyl, hexenoyl, heptanoyl, heptenoyl, octanoyl, octenoyl, nonanoyl,nonenoyl, decanoyl, decenoyl, propionyl, 2-propenoyl, 2-butenoyl,Isobutyryl, 2-methylpropanoyl, 2-ethylbutyryl, ethylbutanoyl,2-ethylbutanoyl, butyryl, (E)-2,3-Dimethylacryloyl,(E)-2-Methylcrotonoyl, 3-cis-Methyl-methacryloyl, 3-Methyl-2-butenoyl,3-Methylcrotonoyl, 4-Pentenoyl, (2E)-2-pentenoyl, Caproyl, 5-Hexenoyl,Capryloyl, Lauroyl, Dodecanoyl, Myristoyl, Tetradecanoyl, Oleoyl, C(2-18) Acyl are interchangeable; wherein the attached group can be thesame group or in combination thereof; wherein the connecting groupbetween the core compound and attached group may be O, S,S(O), S (0)2,C(O), C(O)O, NH, N-alkyl, CH2 or CH2O. In an embodiment, R4 is attachedan O-angeloyl, O-tigloyl, O-senecioyl, O-acetyl, O-Crotonoyl,O-3,3-Dimethylartyloyl, O-Cinnamoyl, O-Pentenoyl, O-Hexanoyl, O-benzoyl,O-Ethylbutyryl, O-alkyl, O-dibenzoyl, O-benzoyl, O-alkanoyl, O-alkenoyl,O-benzoyl alkyl substituted O-alkanoyl, O-alkanoyl substituted phenyl,O-alkenoyl substituted phenyl, O-aryl, O-acyl, O-heterocylic,O-heteroraryl, O-alkenylcarbonyl, O-ethanoyl, O-propanoyl, O-propenoyl,O-butanoyl, O-butenoyl, O-pentanoyl, O-hexenoyl, O-heptanoyl,O-heptenoyl, O-octanoyl, O-octenoyl, O-nonanoyl, O-nonenoyl, O-decanoyl,O-decenoyl, O-propionyl, O-2-propenoyl, O-2-butenoyl, O-Isobutyryl,O-2-methylpropanoyl, O-2-ethylbutyryl, O-ethylbutanoyl,O-2-ethylbutanoyl, O-butyryl, O-(E)-2,3-Dimethylacryloyl,O-(E)-2-Methylcrotonoyl, O-3-cis-Methyl-methacryloyl,O-3-Methyl-2-butenoyl, O-3-Methylcrotonoyl, O-4-Pentenoyl,O-(2E)-2-pentenoyl, O-Caproyl, O-5-Hexenoyl, O-Capryloyl, O-Lauroyl,O-Dodecanoyl, O-Myristoyl, O-Tetradecanoyl, O-Oleoyl, O—C(2-18) Acyl,CH2O-angeloyl, CH2O-tigloyl, CH2O-senecioyl, CH2O-acetyl,CH2O-Crotonoyl, CH2O-3,3-Dimethylartyloyl, CH2O-Cinnamoyl,CH2O-Pentenoyl, CH2O-Hexanoyl, CH2O-benzoyl, CH2O-Ethylbutyryl, CH3,CH2OH, CH2O-alkyl, CH2O-dibenzoyl, CH2O-benzoyl, CH2O-alkanoyl,CH2O-alkenoyl, CH2O-benzoyl alkyl substituted O-alkanoyl, CH2O-alkanoylsubstituted phenyl, CH2O-alkenoyl substituted phenyl, CH2O-aryl,CH2O-acyl, CH2O-heterocylic, CH2O-heteroraryl, CH2O-alkenylcarbonyl,CH2O-ethanoyl, CH2O-propanoyl, CH2O-propenoyl, CH2O-butanoyl,CH2O-butenoyl, CH2O-pentanoyl, CH2O-hexenoyl, CH2O-heptanoyl,CH2O-heptenoyl, CH2O-octanoyl, CH2O-octenoyl, CH2O-nonanoyl,CH2O-nonenoyl, CH2O-decanoyl, CH2O-decenoyl, CH2O-propionyl,CH2O-2-propenoyl, CH2O-2-butenoyl, CH2O-Isobutyryl,CH2O-2-methylpropanoyl, CH2O-2-ethylbutyryl, CH2O-ethylbutanoyl,CH2O-2-ethylbutanoyl, CH2O-butyryl, CH2O-(E)-2,3-Dimethylacryloyl,CH2O-(E)-2-Methylcrotonoyl, CH2O-3-cis-Methyl-methacryloyl,CH2O-3-Methyl-2-butenoyl, CH2O-3-Methylcrotonoyl, CH2O-4-Pentenoyl,CH2O-(2E)-2-pentenoyl, CH2O-Caproyl, CH2O-5-Hexenoyl, CH2O-Capryloyl,CH2O-Lauroyl, CH2O-Dodecanoyl, CH2O-Myristoyl, CH2O-Tetradecanoyl,CH2O-Oleoyl, CH2O—C(2-18) Acyl. In embodiment, the above structures wereattached an O with double bond. In an embodiment, the connecting groupbetween the functional group of angeloyl, tigloyl, senecioyl, acetyl,Crotonoyl, 3,3-Dimethylartyloyl, Cinnamoyl, Pentenoyl, Hexanoyl,benzoyl, Ethylbutyryl, alkyl, dibenzoyl, benzoyl, alkanoyl, alkenoyl,benzoyl alkyl substituted alkanoyl, alkanoyl substituted phenyl,alkenoyl substituted phenyl, aryl, acyl, heterocylic, heteroraryl, andalkenylcarbonyl ethanoyl, propanoyl, propenoyl, butanoyl, butenoyl,pentanoyl, hexenoyl, heptanoyl, heptenoyl, octanoyl, octenoyl, nonanoyl,nonenoyl, decanoyl, decenoyl, propionyl, 2-propenoyl, 2-butenoyl,Isobutyryl, 2-methylpropanoyl, 2-ethylbutyryl, ethylbutanoyl,2-ethylbutanoyl, butyryl, (E)-2,3-Dimethylacryloyl,(E)-2-Methylcrotonoyl, 3-cis-Methyl-methacryloyl, 3-Methyl-2-butenoyl,3-Methylcrotonoyl, 4-Pentenoyl, (2E)-2-pentenoyl, Caproyl, 5-Hexenoyl,Capryloyl, Lauroyl, Dodecanoyl, Myristoyl, Tetradecanoyl, Oleoyl,C(2-18) Acyl can be O, S, S(O), S(O)2, C(O), C(O)O, NH, N-alkyl, CH2 orCH2O. In an embodiment, wherein any 1 or 2 or 3 or 4 or 5 or 6 of R1,R2, R3, R4, R5, R8, R9, R10, R11, R12, R13, R14, R15 are independentlyselected from the group of A-B, wherein A can be O, S, S(O), S(O)2,C(O), C(O)O, NH, N-alkyl, CH2 or CH2O; wherein B is selected from thegroup of acetyl, angeloyl, tigloyl, senecioyl, Crotonoyl,3,3-Dimethylartyloyl, Cinnamoyl, Pentenoyl, Hexanoyl, benzoyl,Ethylbutyryl, alkyl, dibenzoyl, benzoyl, methylbutanoyl,methylpropanoyl, alkanoyl, alkenoyl, benzoyl alkyl substituted alkanoyl,alkanoyl substituted phenyl, alkenoyl substituted phenyl, aryl, acyl,heterocylic, heteroraryl, alkenylcarbonyl, ethanoyl, propanoyl,propenoyl, butanoyl, butenoyl, pentanoyl, hexenoyl, heptanoyl,heptenoyl, octanoyl, octenoyl, nonanoyl, nonenoyl, decanoyl, decenoyl,propionyl, 2-propenoyl, 2-butenoyl, Isobutyryl, 2-methylpropanoyl,2-ethylbutyryl, ethylbutanoyl, 2-ethylbutanoyl, butyryl,(E)-2,3-Dimethylacryloyl, (E)-2-Methylcrotonoyl,3-cis-Methyl-methacryloyl, 3-Methyl-2-butenoyl, 3-Methylcrotonoyl,4-Pentenoyl, (2E)-2-pentenoyl, Caproyl, 5-Hexenoyl, Capryloyl, Lauroyl,Dodecanoyl, Myristoyl, Tetradecanoyl, Oleoyl and C(2-18) Acyl. In anenbodiment, R1 is A-B. In an enbodiment, R2 is A-B. In an enbodiment, R3is A-B. In an enbodiment, R4 is A-B. In an enbodiment, R5 is A-B. In anenbodiment, R6 is A-B. In an enbodiment, R7 is A-B. In an enbodiment, R8is A-B. In an enbodiment, R9 is A-B. In an enbodiment, R10 is A-B. In anenbodiment, R11 is A-B. In an enbodiment, R12 is A-B.

In an enbodiment, R13 is A-B. In an enbodiment, R14 is A-B. In anenbodiment, R15 is A-B. In embodiment, the compound is attached a sugarmoiety(ies), acid moiety(ies) or alduronic acid. In an embodiment, thecompound is selected from the structure:

R1, R2, R3, R4, R5, R8, R9, R10, R11, R12, R13, R14, R15, R16 areindependently selected from the group of H, O, OH, CH3, CH2OH, COOH,hydrogen, hydroxyl, methyl, O-angeloyl, O-tigloyl, O-senecioyl,O-acetyl, O-Crotonoyl, O-3,3-Dimethylartyloyl, O-Cinnamoyl, O-Pentenoyl,O-Hexanoyl, O-benzoyl, O-Ethylbutyryl, O-alkyl, O-dibenzoyl, O-benzoyl,O-alkanoyl, O-alkenoyl, O-benzoyl alkyl substituted O-alkanoyl,O-alkanoyl substituted phenyl, O-alkenoyl substituted phenyl, O-aryl,O-acyl, O-heterocylic, O-heteroraryl, O-alkenylcarbonyl, O-alkane,O-alkene, O-sugar moiety, O-acid moiety, O-ethanoyl, O-propanoyl,O-propenoyl, O-butanoyl, O-butenoyl, O-pentanoyl, O-hexenoyl,O-heptanoyl, O-heptenoyl, O-octanoyl, O-octenoyl, O-nonanoyl,O-nonenoyl, O-decanoyl, O-decenoyl, O-propionyl, O-2-propenoyl,O-2-butenoyl, O-Isobutyryl, O-2-methylpropanoyl, O-2-ethylbutyryl,O-ethylbutanoyl, O-2-ethylbutanoyl, O-butyryl,O-(E)-2,3-Dimethylacryloyl, O-(E)-2-Methylcrotonoyl,O-3-cis-Methyl-methacryloyl, O-3-Methyl-2-butenoyl, O-3-Methylcrotonoyl,O-4-Pentenoyl, O-(2E)-2-pentenoyl, O-Caproyl, O-5-Hexenoyl, O-Capryloyl,O-Lauroyl, O-Dodecanoyl, O-Myristoyl, O-Tetradecanoyl, O-Oleoyl,O—C(2-18) Acyl, COO-angeloyl, COO-tigloyl, COO-senecioyl, COO-acetyl,COO-Crotonoyl, COO-3,3-Dimethylartyloyl, COO-Cinnamoyl, COO-Pentenoyl,COO-Hexanoyl, COO-benzoyl, COO-Ethylbutyryl, COO-alkyl, COO-dibenzoyl,COO-benzoyl, COO-alkanoyl, CH2O-alkenoyl, COO-benzoyl alkyl substitutedO-alkanoyl, COO-alkanoyl substituted phenyl, COO-alkenoyl substitutedphenyl, COO-aryl, COO-acyl, COO-heterocylic, COO-heteroraryl,COO-alkenylcarbonyl, COO-alkane, COO-alkene, COO-sugar moiety, COO-acidmoiety, COO-ethanoyl, COO-propanoyl, COO-propenoyl, COO-butanoyl,COO-butenoyl, COO-pentanoyl, COO-hexenoyl, COO-heptanoyl, COO-heptenoyl,COO-octanoyl, COO-octenoyl, COO-nonanoyl, COO-nonenoyl, COO-decanoyl,COO-decenoyl, COO-propionyl, COO-2-propenoyl, COO-2-butenoyl,COO-Isobutyryl, COO-2-methylpropanoyl, COO-2-ethylbutyryl,COO-ethylbutanoyl, COO-2-ethylbutanoyl, COO-butyryl,COO-(E)-2,3-Dimethylacryloyl, COO-(E)-2-Methylcrotonoyl,COO-3-cis-Methyl-methacryloyl, COO-3-Methyl-2-butenoyl,COO-3-Methylcrotonoyl, COO-4-Pentenoyl, COO-(2E)-2-pentenoyl,COO-Caproyl, COO-5-Hexenoyl, COO-Capryloyl, COO-Lauroyl, COO-Dodecanoyl,COO-Myristoyl, COO-Tetradecanoyl, COO-Oleoyl, COO—C(2-18) Acyl,CH2O-angeloyl, CH2O-tigloyl, CH2O-senecioyl, CH2O-acetyl,CH2O-Crotonoyl, CH2O-3,3-Dimethylartyloyl, CH2O-Cinnamoyl,CH2O-Pentenoyl, CH2O-Hexanoyl, CH2O-benzoyl, CH2O-Ethylbutyryl,CH2O-alkyl, CH2O-dibenzoyl, CH2O-benzoyl, CH2O-alkanoyl, CH2O-alkenoyl,CH2O-benzoyl alkyl substituted O-alkanoyl, CH2O-alkanoyl substitutedphenyl, CH2O-alkenoyl substituted phenyl, CH2O-aryl, CH2O-acyl,CH2O-heterocylic, CH2O-heteroraryl, CH2O-alkenylcarbonyl, CH2O-alkane,CH2O-alkene and CH2O-sugar moiety, CH2O-angeloyl, CH2O-tigloyl,CH2O-senecioyl, CH2O-acetyl, CH2O-Crotonoyl, CH2O-3,3-Dimethylartyloyl,CH2O-Cinnamoyl, CH2O-Pentenoyl, CH2O-Hexanoyl, CH2O-benzoyl,CH2O-Ethylbutyryl, CH3, CH2OH, CH2O-alkyl, CH2O-dibenzoyl, CH2O-benzoyl,CH2O-alkanoyl, CH2O-alkenoyl, CH2O-benzoyl alkyl substituted O-alkanoyl,CH2O-alkanoyl substituted phenyl, CH2O-alkenoyl substituted phenyl,CH2O-aryl, CH2O-acyl, CH2O-heterocylic, CH2O-heteroraryl,CH2O-alkenylcarbonyl, CH2O-ethanoyl, CH2O-propanoyl, CH2O-propenoyl,CH2O-butanoyl, CH2O-butenoyl, CH2O-pentanoyl, CH2O-hexenoyl,CH2O-heptanoyl, CH2O-heptenoyl, CH2O-octanoyl, CH2O-octenoyl,CH2O-nonanoyl, CH2O-nonenoyl, CH2O-decanoyl, CH2O-decenoyl,CH2O-propionyl, CH2O-2-propenoyl, CH2O-2-butenoyl, CH2O-Isobutyryl,CH2O-2-methylpropanoyl, CH2O-2-ethylbutyryl, CH2O-ethylbutanoyl,CH2O-2-ethylbutanoyl, CH2O-butyryl, CH2O-(E)-2,3-Dimethylacryloyl,CH2O-(E)-2-Methylcrotonoyl, CH2O-3-cis-Methyl-methacryloyl,CH2O-3-Methyl-2-butenoyl, CH2O-3-Methylcrotonoyl, CH2O-4-Pentenoyl,CH2O-(2E)-2-pentenoyl, CH2O-Caproyl, CH2O-5-Hexenoyl, CH2O-Capryloyl,CH2O-Lauroyl, CH2O-Dodecanoyl, CH2O-Myristoyl, CH2O-Tetradecanoyl,CH2O-Oleoyl, CH2O—C(2-18) Acyl, (CnH2n)O-angeloyl, (CnH2n)O-tigloyl,(CnH2n)O-senecioyl, (CnH2n)O-acetyl, (CnH2n)O-Crotonoyl,(CnH2n)O-3,3-Dimethylartyloyl, (CnH2n)O-Cinnamoyl, (CnH2n)O-Pentenoyl,(CnH2n)O-Hexanoyl, (CnH2n)O-benzoyl, (CnH2n)O-Ethylbutyryl,(CnH2n)O-alkyl, (CnH2n)O-dibenzoyl, (CnH2n)O-benzoyl, (CnH2n)O-alkanoyl,(CnH2n)O-alkenoyl, (CnH2n)O-benzoyl alkyl substituted O-alkanoyl,(CnH2n)O-alkanoyl substituted phenyl, (CnH2n)O-alkenoyl substitutedphenyl, (CnH2n)O-aryl, (CnH2n)O-acyl, (CnH2n)O-heterocylic,(CnH2n)O-heteroraryl, (CnH2n)O-alkenylcarbonyl, (CnH2n)O-alkane,(CnH2n)O-alkene and (CnH2n)O-sugar moiety and (CnH2n)O-acid moiety,wherein n is 1 or 2 or 3 or 4 or over 5 or derivatives thereof; whereinthe sugar moiety(ies) is/are selected from a group of glucose,galactose, rhamnose, arabinose, xylose, fucose, allose, altrose, gulose,idose, lyxose, mannose, psicose, ribose, sorbose, tagatose, talose,fructose, alduronic acid, glucuronic acid, galacturonic acid, andderivatives or combinations thereof; or wherein any 1 or 2 or 3 or 4 ofR1, R2, R3, R4, R5, R8, R16 and R10 are independently attached anO-angeloyl, O-tigloyl, O-senecioyl, O-acetyl, O-Crotonoyl,O-3,3-Dimethylartyloyl, O-Cinnamoyl, O-Pentenoyl, O-Hexanoyl, O-benzoyl,O-Ethylbutyryl, O-alkyl, O-dibenzoyl, O-benzoyl, O-alkanoyl, O-alkenoyl,O-benzoyl alkyl substituted O-alkanoyl, O-alkanoyl substituted phenyl,O-alkenoyl substituted phenyl, O-aryl, O-acyl, O-heterocylic,O-heteroraryl, O-alkenylcarbonyl, O-ethanoyl, O-propanoyl, O-propenoyl,O-butanoyl, O-butenoyl, O-pentanoyl, O-hexenoyl, O-heptanoyl,O-heptenoyl, O-octanoyl, O-octenoyl, O-nonanoyl, O-nonenoyl, O-decanoyl,O-decenoyl, O-propionyl, O-2-propenoyl, O-2-butenoyl, O-Isobutyryl,O-2-methylpropanoyl, O-2-ethylbutyryl, O-ethylbutanoyl,O-2-ethylbutanoyl, O-butyryl, O-(E)-2,3-Dimethylacryloyl,O-(E)-2-Methylcrotonoyl, O-3-cis-Methyl-methacryloyl,O-3-Methyl-2-butenoyl, O-3-Methylcrotonoyl, O-4-Pentenoyl,O-(2E)-2-pentenoyl, O-Caproyl, O-5-Hexenoyl, O-Capryloyl, O-Lauroyl,O-Dodecanoyl, O-Myristoyl, O-Tetradecanoyl, O-Oleoyl, O—C(2-18) Acyl,CH2O-angeloyl, CH2O-tigloyl, CH2O-senecioyl, CH2O-acetyl,CH2O-Crotonoyl, CH2O-3,3-Dimethylartyloyl, CH2O-Cinnamoyl,CH2O-Pentenoyl, CH2O-Hexanoyl, CH2O-benzoyl, CH2O-Ethylbutyryl, CH3,CH2OH, CH2O-alkyl, CH2O-dibenzoyl, CH2O-benzoyl, CH2O-alkanoyl,CH2O-alkenoyl, CH2O-benzoyl alkyl substituted O-alkanoyl, CH2O-alkanoylsubstituted phenyl, CH2O-alkenoyl substituted phenyl, CH2O-aryl,CH2O-acyl, CH2O-heterocylic, CH2O-heteroraryl, CH2O-alkenylcarbonyl,CH2O-ethanoyl, CH2O-propanoyl, CH2O-propenoyl, CH2O-butanoyl,CH2O-butenoyl, CH2O-pentanoyl, CH2O-hexenoyl, CH2O-heptanoyl,CH2O-heptenoyl, CH2O-octanoyl, CH2O-octenoyl, CH2O-nonanoyl,CH2O-nonenoyl, CH2O-decanoyl, CH2O-decenoyl, CH2O-propionyl,CH2O-2-propenoyl, CH2O-2-butenoyl, CH2O-Isobutyryl,CH2O-2-methylpropanoyl, CH2O-2-ethylbutyryl, CH2O-ethylbutanoyl,CH2O-2-ethylbutanoyl, CH2O-butyryl, CH2O-(E)-2,3-Dimethylacryloyl,CH2O-(E)-2-Methylcrotonoyl, CH2O-3-cis-Methyl-methacryloyl,CH2O-3-Methyl-2-butenoyl, CH2O-3-Methylcrotonoyl, CH2O-4-Pentenoyl,CH2O-(2E)-2-pentenoyl, CH2O-Caproyl, CH2O-5-Hexenoyl, CH2O-Capryloyl,CH2O-Lauroyl, CH2O-Dodecanoyl, CH2O-Myristoyl, CH2O-Tetradecanoyl,CH2O-Oleoyl, CH2O—C(2-18) Acyl; or wherein R9, R11, R12, R13, R14, R15,are independently attached a CH3; or wherein R10 is attached anO-angeloyl, O-tigloyl, O-senecioyl, O-acetyl, O-Crotonoyl,O-3,3-Dimethylartyloyl, O-Cinnamoyl, O-Pentenoyl, O-Hexanoyl, O-benzoyl,O-Ethylbutyryl, O-alkyl, O-dibenzoyl, O-benzoyl, O-alkanoyl, O-alkenoyl,O-benzoyl alkyl substituted O-alkanoyl, O-alkanoyl substituted phenyl,O-alkenoyl substituted phenyl, O-aryl, O-acyl, O-heterocylic,O-heteroraryl, O-alkenylcarbonyl, O-ethanoyl, O-propanoyl, O-propenoyl,O-butanoyl, O-butenoyl, O-pentanoyl, O-hexenoyl, O-heptanoyl,O-heptenoyl, O-octanoyl, O-octenoyl, O-nonanoyl, O-nonenoyl, O-decanoyl,O-decenoyl, O-propionyl, O-2-propenoyl, O-2-butenoyl, O-Isobutyryl,O-2-methylpropanoyl, O-2-ethylbutyryl, O-ethylbutanoyl,O-2-ethylbutanoyl, O-butyryl, O-(E)-2,3-Dimethylacryloyl,O-(E)-2-Methylcrotonoyl, O-3-cis-Methyl-methacryloyl,O-3-Methyl-2-butenoyl, O-3-Methylcrotonoyl, O-4-Pentenoyl,O-(2E)-2-pentenoyl, O-Caproyl, O-5-Hexenoyl, O-Capryloyl, O-Lauroyl,O-Dodecanoyl, O-Myristoyl, O-Tetradecanoyl, O-Oleoyl, O—C(2-18) Acyl,CH2O-angeloyl, CH2O-tigloyl, CH2O-senecioyl, CH2O-acetyl,CH2O-Crotonoyl, CH2O-3,3-Dimethylartyloyl, CH2O-Cinnamoyl,CH2O-Pentenoyl, CH2O-Hexanoyl, CH2O-benzoyl, CH2O-Ethylbutyryl, CH3,CH2OH, CH2O-alkyl, CH2O-dibenzoyl, CH2O-benzoyl, CH2O-alkanoyl,CH2O-alkenoyl, CH2O-benzoyl alkyl substituted O-alkanoyl, CH2O-alkanoylsubstituted phenyl, CH2O-alkenoyl substituted phenyl, CH2O-aryl,CH2O-acyl, CH2O-heterocylic, CH2O-heteroraryl, CH2O-alkenylcarbonyl,CH2O-ethanoyl, CH2O-propanoyl, CH2O-propenoyl, CH2O-butanoyl,CH2O-butenoyl, CH2O-pentanoyl, CH2O-hexenoyl, CH2O-heptanoyl,CH2O-heptenoyl, CH2O-octanoyl, CH2O-octenoyl, CH2O-nonanoyl,CH2O-nonenoyl, CH2O-decanoyl, CH2O-decenoyl, CH2O-propionyl,CH2O-2-propenoyl, CH2O-2-butenoyl, CH2O-Isobutyryl,CH2O-2-methylpropanoyl, CH2O-2-ethylbutyryl, CH2O-ethylbutanoyl,CH2O-2-ethylbutanoyl, CH2O-butyryl, CH2O-(E)-2,3-Dimethylacryloyl,CH2O-(E)-2-Methylcrotonoyl, CH2O-3-cis-Methyl-methacryloyl,CH2O-3-Methyl-2-butenoyl, CH2O-3-Methylcrotonoyl, CH2O-4-Pentenoyl,CH2O-(2E)-2-pentenoyl, CH2O-Caproyl, CH2O-5-Hexenoyl, CH2O-Capryloyl,CH2O-Lauroyl, CH2O-Dodecanoyl, CH2O-Myristoyl, CH2O-Tetradecanoyl,CH2O-Oleoyl, CH2O—C(2-18) Acyl; or wherein R4 and/or R10 areindependently attached an O-angeloyl, O-tigloyl, O-senecioyl, O-acetyl,O-Crotonoyl, O-3,3-Dimethylartyloyl, O-Cinnamoyl, O-Pentenoyl,O-Hexanoyl, O-benzoyl, O-Ethylbutyryl, O-alkyl, O-dibenzoyl, O-benzoyl,O-alkanoyl, O-alkenoyl, O-benzoyl alkyl substituted O-alkanoyl,O-alkanoyl substituted phenyl, O-alkenoyl substituted phenyl, O-aryl,O-acyl, O-heterocylic, O-heteroraryl, O-alkenylcarbonyl, O-ethanoyl,O-propanoyl, O-propenoyl, O-butanoyl, O-butenoyl, O-pentanoyl,O-hexenoyl, O-heptanoyl, O-heptenoyl, O-octanoyl, O-octenoyl,O-nonanoyl, O-nonenoyl, O-decanoyl, O-decenoyl, O-propionyl,O-2-propenoyl, O-2-butenoyl, O-Isobutyryl, O-2-methylpropanoyl,O-2-ethylbutyryl, O-ethylbutanoyl, O-2-ethylbutanoyl, O-butyryl,O-(E)-2,3-Dimethylacryloyl, O-(E)-2-Methylcrotonoyl,O-3-cis-Methyl-methacryloyl, O-3-Methyl-2-butenoyl, O-3-Methylcrotonoyl,O-4-Pentenoyl, O-(2E)-2-pentenoyl, O-Caproyl, O-5-Hexenoyl, O-Capryloyl,O-Lauroyl, O-Dodecanoyl, O-Myristoyl, O-Tetradecanoyl, O-Oleoyl,O—C(2-18) Acyl, CH2O-angeloyl, CH2O-tigloyl, CH2O-senecioyl,CH2O-acetyl, CH2O-Crotonoyl, CH2O-3,3-Dimethylartyloyl, CH2O-Cinnamoyl,CH2O-Pentenoyl, CH2O-Hexanoyl, CH2O-benzoyl, CH2O-Ethylbutyryl, CH3,CH2OH, CH2O-alkyl, CH2O-dibenzoyl, CH2O-benzoyl, CH2O-alkanoyl,CH2O-alkenoyl, CH2O-benzoyl alkyl substituted O-alkanoyl, CH2O-alkanoylsubstituted phenyl, CH2O-alkenoyl substituted phenyl, CH2O-aryl,CH2O-acyl, CH2O-heterocylic, CH2O-heteroraryl, CH2O-alkenylcarbonyl,CH2O-ethanoyl, CH2O-propanoyl, CH2O-propenoyl, CH2O-butanoyl,CH2O-butenoyl, CH2O-pentanoyl, CH2O-hexenoyl, CH2O-heptanoyl,CH2O-heptenoyl, CH2O-octanoyl, CH2O-octenoyl, CH2O-nonanoyl,CH2O-nonenoyl, CH2O-decanoyl, CH2O-decenoyl, CH2O-propionyl,CH2O-2-propenoyl, CH2O-2-butenoyl, CH2O-Isobutyryl,CH2O-2-methylpropanoyl, CH2O-2-ethylbutyryl, CH2O-ethylbutanoyl,CH2O-2-ethylbutanoyl, CH2O-butyryl, CH2O-(E)-2,3-Dimethylacryloyl,CH2O-(E)-2-Methylcrotonoyl, CH2O-3-cis-Methyl-methacryloyl,CH2O-3-Methyl-2-butenoyl, CH2O-3-Methylcrotonoyl, CH2O-4-Pentenoyl,CH2O-(2E)-2-pentenoyl, CH2O-Caproyl, CH2O-5-Hexenoyl, CH2O-Capryloyl,CH2O-Lauroyl, CH2O-Dodecanoyl, CH2O-Myristoyl, CH2O-Tetradecanoyl,CH2O-Oleoyl, CH2O—C(2-18) Acyl; wherein R3 is OH or H or absent; whereinR1, R2, R3, R5, R8, R16 are OH or H or absent; or wherein an O wereattached to the above structures with double bond; wherein R9, R11, R12,R13, R14, and R15 are CH3; or wherein R1, R2, R5, R8 represent OH; R3represents OH, H or absent; or wherein R4, R10 represent CH2Oangeloyl;R9, R11, R12, R13, R14, R15 represent CH3; or wherein R1, R2, R5, R8represent OH or O-tigloyl; R3 represents OH, H, or absent; or whereinR4, R10 represent CH2O tigloyl; R9, R11, R12, R13, R14, R15 representCH3; wherein the group attaching to the core compound selected fromacetyl, angeloyl, tigloyl, senecioyl, Crotonoyl, 3,3-Dimethylartyloyl,Cinnamoyl, Pentenoyl, Hexanoyl, benzoyl, Ethylbutyryl, alkyl, dibenzoyl,benzoyl, methylbutanoyl, methylpropanoyl, alkanoyl, alkenoyl, benzoylalkyl substituted alkanoyl, alkanoyl substituted phenyl, alkenoylsubstituted phenyl, aryl, acyl, heterocylic, heteroraryl,alkenylcarbonyl, ethanoyl, propanoyl, propenoyl, butanoyl, butenoyl,pentanoyl, hexenoyl, heptanoyl, heptenoyl, octanoyl, octenoyl, nonanoyl,nonenoyl, decanoyl, decenoyl, propionyl, 2-propenoyl, 2-butenoyl,Isobutyryl, 2-methyl propanoyl, 2-ethylbutyryl, ethylbutanoyl,2-ethylbutanoyl, butyryl, (E)-2,3-Dimethylacryloyl,(E)-2-Methylcrotonoyl, 3-cis-Methyl-methacryloyl, 3-Methyl-2-butenoyl,3-Methylcrotonoyl, 4-Pentenoyl, (2E)-2-pentenoyl, Caproyl, 5-Hexenoyl,Capryloyl, Lauroyl, Dodecanoyl, Myristoyl, Tetradecanoyl, Oleoyl,C(2-18) Acyl are interchangeable; wherein the attached group can be thesame group or in combination thereof; wherein the connecting groupbetween the core compound and attached group may be O, S, S(O), S(O)2,C(O), C(O)O, NH, N-alkyl, CH2 or CH2O. In an embodiment, R4 is attachedan O-angeloyl, O-tigloyl, O-senecioyl, O-acetyl, O-Crotonoyl,O-3,3-Dimethylartyloyl, O-Cinnamoyl, O-Pentenoyl, O-Hexanoyl, O-benzoyl,O-Ethylbutyryl, O-alkyl, O-dibenzoyl, O-benzoyl, O-alkanoyl, O-alkenoyl,O-benzoyl alkyl substituted O-alkanoyl, O-alkanoyl substituted phenyl,O-alkenoyl substituted phenyl, O-aryl, O-acyl, O-heterocylic,O-heteroraryl, O-alkenylcarbonyl, O-ethanoyl, O-propanoyl, O-propenoyl,O-butanoyl, O-butenoyl, O-pentanoyl, O-hexenoyl, O-heptanoyl,O-heptenoyl, O-octanoyl, O-octenoyl, O-nonanoyl, O-nonenoyl, O-decanoyl,O-decenoyl, O-propionyl, O-2-propenoyl, O-2-butenoyl, O-Isobutyryl,O-2-methylpropanoyl, O-2-ethylbutyryl, O-ethylbutanoyl,O-2-ethylbutanoyl, O-butyryl, O-(E)-2,3-Dimethylacryloyl,O-(E)-2-Methylcrotonoyl, O-3-cis-Methyl-methacryloyl,O-3-Methyl-2-butenoyl, O-3-Methylcrotonoyl, O-4-Pentenoyl,O-(2E)-2-pentenoyl, O-Caproyl, O-5-Hexenoyl, O-Capryloyl, O-Lauroyl,O-Dodecanoyl, O-Myristoyl, O-Tetradecanoyl, O-Oleoyl, O—C(2-18) Acyl,CH2O-angeloyl, CH2O-tigloyl, CH2O-senecioyl, CH2O-acetyl,CH2O-Crotonoyl, CH2O-3,3-Dimethylartyloyl, CH2O-Cinnamoyl,CH2O-Pentenoyl, CH2O-Hexanoyl, CH2O-benzoyl, CH2O-Ethylbutyryl, CH3,CH2OH, CH2O-alkyl, CH2O-dibenzoyl, CH2O-benzoyl, CH2O-alkanoyl,CH2O-alkenoyl, CH2O-benzoyl alkyl substituted O-alkanoyl, CH2O-alkanoylsubstituted phenyl, CH2O-alkenoyl substituted phenyl, CH2O-aryl,CH2O-acyl, CH2O-heterocylic, CH2O-heteroraryl, CH2O-alkenylcarbonyl,CH2O-ethanoyl, CH2O-propanoyl, CH2O-propenoyl, CH2O-butanoyl,CH2O-butenoyl, CH2O-pentanoyl, CH2O-hexenoyl, CH2O-heptanoyl,CH2O-heptenoyl, CH2O-octanoyl, CH2O-octenoyl, CH2O-nonanoyl,CH2O-nonenoyl, CH2O-decanoyl, CH2O-decenoyl, CH2O-propionyl,CH2O-2-propenoyl, CH2O-2-butenoyl, CH2O-Isobutyryl,CH2O-2-methylpropanoyl, CH2O-2-ethylbutyryl, CH2O-ethylbutanoyl,CH2O-2-ethylbutanoyl, CH2O-butyryl, CH2O-(E)-2,3-Dimethylacryloyl,CH2O-(E)-2-Methylcrotonoyl, CH2O-3-cis-Methyl-methacryloyl,CH2O-3-Methyl-2-butenoyl, CH2O-3-Methylcrotonoyl, CH2O-4-Pentenoyl,CH2O-(2E)-2-pentenoyl, CH2O-Caproyl, CH2O-5-Hexenoyl, CH2O-Capryloyl,CH2O-Lauroyl, CH2O-Dodecanoyl, CH2O-Myristoyl, CH2O-Tetradecanoyl,CH2O-Oleoyl, CH2O—C(2-18) Acyl, In an embodiment, the connecting groupbetween the functional group of angeloyl, tigloyl, senecioyl, acetyl,Crotonoyl, 3,3-Dimethylartyloyl, Cinnamoyl, Pentenoyl, Hexanoyl,benzoyl, Ethylbutyryl, alkyl, dibenzoyl, benzoyl, alkanoyl, alkenoyl,benzoyl alkyl substituted alkanoyl, alkanoyl substituted phenyl,alkenoyl substituted phenyl, aryl, acyl, heterocylic, heteroraryl, andalkenylcarbonyl ethanoyl, propanoyl, propenoyl, butanoyl, butenoyl,pentanoyl, hexenoyl, heptanoyl, heptenoyl, octanoyl, octenoyl, nonanoyl,nonenoyl, decanoyl, decenoyl, propionyl, 2-propenoyl, 2-butenoyl,Isobutyryl, 2-methylpropanoyl, 2-ethylbutyryl, ethylbutanoyl,2-ethylbutanoyl, butyryl, (E)-2,3-Dimethylacryloyl,(E)-2-Methylcrotonoyl, 3-cis-Methyl-methacryloyl, 3-Methyl-2-butenoyl,3-Methylcrotonoyl, 4-Pentenoyl, (2E)-2-pentenoyl, Caproyl, 5-Hexenoyl,Capryloyl, Lauroyl, Dodecanoyl, Myristoyl, Tetradecanoyl, Oleoyl,C(2-18) Acyl can be O, S, S(O), S(O)2, C(O), C(O)O, NH, N-alkyl, CH2 orCH2O. In an embodiment, any 1 or 2 or 3 or 4 or 5 or 6 of R1, R2, R3,R4, R5, R8, R9, R10, R11, R12, R13, R14, R15, R16 are independentlyselected from the group of A-B, wherein A can be O, S, S(O), S(O)2,C(O), C(O)O, NH, N-alkyl, CH2 or CH2O; wherein B is selected from thegroup of acetyl, angeloyl, tigloyl, senecioyl, Crotonoyl,3,3-Dimethylartyloyl, Cinnamoyl, Pentenoyl, Hexanoyl, benzoyl,Ethylbutyryl, alkyl, dibenzoyl, benzoyl, methylbutanoyl,methylpropanoyl, alkanoyl, alkenoyl, benzoyl alkyl substituted alkanoyl,alkanoyl substituted phenyl, alkenoyl substituted phenyl, aryl, acyl,heterocylic, heteroraryl, alkenylcarbonyl, ethanoyl, propanoyl,propenoyl, butanoyl, butenoyl, pentanoyl, hexenoyl, heptanoyl,heptenoyl, octanoyl, octenoyl, nonanoyl, nonenoyl, decanoyl, decenoyl,propionyl, 2-propenoyl, 2-butenoyl, Isobutyryl, 2-methylpropanoyl,2-ethylbutyryl, ethylbutanoyl, 2-ethylbutanoyl, butyryl,(E)-2,3-Dimethylacryloyl, (E)-2-Methylcrotonoyl,3-cis-Methyl-methacryloyl, 3-Methyl-2-butenoyl, 3-Methylcrotonoyl,4-Pentenoyl, (2E)-2-pentenoyl, Caproyl, 5-Hexenoyl, Capryloyl, Lauroyl,Dodecanoyl, Myristoyl, Tetradecanoyl, Oleoyl and C(2-18) Acyl. In anembodiment, R1 is A-B. In an embodiment, R2 is A-B. In an embodiment, R3is A-B. In an embodiment, R4 is A-B. In an embodiment, R5 is A-B. In anembodiment, R6 is A-B. In an embodiment, R7 is A-B. In an embodiment, R8is A-B. In an embodiment, R9 is A-B. In an embodiment, R10 is A-B. In anembodiment, R11 is A-B. In an embodiment, R12 is A-B. In an embodiment,R13 is A-B. In an embodiment, R14 is A-B. In an embodiment, R15 is A-B.In an embodiment, this invention provides compounds of the above toimprove blood circulation; soothing stroke; Prevent plaque formation andpromote their dissipated; improve blood viscosity; reducecardiovascular; reduce cerebrovascular; reduce thrombosis,arteriosclerosis, coronary heart disease, hypertension, diabetes,thrombocytopenia purpura, hemoptysis, hematemesis; treating blood in thestool, uterine bleeding, traumatic bleeding, abdominal irritation,swelling, flutter, Blood circulation, swelling, pain; Treatingbronchiectasis, tuberculosis and lung abscess caused by too hemoptysis;reducing bleeding, antitussive, expectorant and analgesic effect, dilateblood vessels; reducing blood pressure and the treatment of cerebralarteriosclerosis; elevated blood lipids and reduced cholesterol. The R1,R2, R3, R4, R5, R8, R9, R10, R11, R12, R13, R14, R15, R16 bonds of (3K2)can be alpha or beta.

R1, R2, R3, R4, R5, R8, R9, R10, R11, R12, R13, R14, R15, R16 areindependently selected from the group of 0, hydrogen, hydroxyl, methyl,O-angeloyl, O-tigloyl, O-senecioyl, O-acetyl, O-Crotonoyl,O-3,3-Dimethylartyloyl, O-Cinnamoyl, O-Pentenoyl, O-Hexanoyl, O-benzoyl,O-Ethylbutyryl, O-alkyl, O-dibenzoyl, O-benzoyl, O-alkanoyl, O-alkenoyl,O-benzoyl alkyl substituted O-alkanoyl, O-alkanoyl substituted phenyl,O-alkenoyl substituted phenyl, O-aryl, O-acyl, O-heterocylic,O-heteroraryl, O-alkenylcarbonyl, O-ethanoyl, O-propanoyl, O-propenoyl,O-butanoyl, O-butenoyl, O-pentanoyl, O-hexenoyl, O-heptanoyl,O-heptenoyl, O-octanoyl, O-octenoyl, O-nonanoyl, O-nonenoyl, O-decanoyl,O-decenoyl, O-propionyl, O-2-propenoyl, O-2-butenoyl, O-Isobutyryl,O-2-methylpropanoyl, O-2-ethylbutyryl, O-ethylbutanoyl,O-2-ethylbutanoyl, O-butyryl, O-(E)-2,3-Dimethylacryloyl,O-(E)-2-Methylcrotonoyl, O-3-cis-Methyl-methacryloyl,O-3-Methyl-2-butenoyl, O-3-Methylcrotonoyl, O-4-Pentenoyl,O-(2E)-2-pentenoyl, O-Caproyl, O-5-Hexenoyl, O-Capryloyl, O-Lauroyl,O-Dodecanoyl, O-Myristoyl, O-Tetradecanoyl, O-Oleoyl,

In embodiment the core having structures:

wherein R1, R2, R3, R4, R5, R8, R9, R10, R11, R12, R13, R14, R15, R16,R17, R18, of T19, T20, T21, T22, T23, T24, T25, T26, T27 represent H,OH, O, CH2OH, COOH or CH3. The bonds can be in forms of alpha or beta orin combinations.

Esterification the above core compound with acyl halide, wherein thehalide comprise chloride, bromide, fluoride and iodide, wherein the acylhalide comprise acyl chloride, wherein acyl chloride comprise tigloylchloride, angeloyl chloride, acetyl chloride, crotonoyl chloride,3,3-dimethylartyloyl chloride, senecioyl chloride, cinnamoyl chloride,pentenoyl chloride, hexanoyl chloride, benzoyl chloride, ethylbutyrylchloride, propionyl chloride, 2-propenoyl chloride, isobutyryl chloride,butyryl chloride, (2E)-2-pentenoyl chloride, 4-Pentenoyl chloride,5-hexenoyl chloride, heptanoyl chloride, octanoyl chloride, nonanoylchloride, decanoyl chloride, Lauroyl chloride, myristoyl chloride,oleoyl chloride. The compounds vary in composition when the time ortemperature of the reaction is changed. The peaks, fractions andcompounds are selected according to the activities of times studies andthe changes of peaks. The compounds having strong to weak activities areselected and isolated. The anti cancer activities (Cytotoxic Assay) arethe MTT studies of bone (U2OS), lung (H460), bladder(HTB-9), ovary(ES2), colon (HCT116), pancreas (Capan), ovary (OVCAR3), prostate(DU145), skin (SK-Mel-5), mouth (KB), kidney (A498), breast (MCF-7),liver (HepG2), brain (T98G), luekemia (K562), cervix (HeLa). The activeesterification products are purified with HPLC. The reaction product ofmixtures and individual compounds are tested with MTT Cytotoxic Assay.Details of method are in Experiment 3 of the present application. Asecond esterification of compound can be selected from the aboveexperiment results to produce new active compounds. A partialesterification compound is selected from the above experiments toperform a second or repeated with a third esterification with differentacyl chloride in order to produce new active compounds with theexperiments in the present application.

A method is 1) Dissolving core compound or triterpenes core,hydroxylated triterpenes core, in pyridine; 2) Adding acyl halide oracyl chloride; 3, The mixture is stirred for length of time including 5sec, 10 sec, 20 sec, 30 sec, 40 sec, 1 min, 2 min, 5 min, 10 min, 30min, 1 hr, 2 hr, 18 hr, 2 days or 3 days at different temperature; 4) Atthe end of reaction, aqueous solution of acid or weak base, or water isadded to the reaction mixture; 5) The solution is then extracted ofethyl acetate and ethyl acetate is removed by evaporation andlyophilization; 6) Dissolving the reaction product in acetonitrile withTrifluoroacetic acid or DMSO; 7) Testing the reaction product ofmixtures and individual fractions with MTT cytotoxic assay; 8) Selectingthe HPLC fractions for isolation is according to the cytotoxic activityof the reaction product obtained at a specific reaction time; 10)Purifiing the active esterification products with HPLC; 11) Collectingthe products; 12) Testing the products; wherein the core compound isterpene, isoprene, or triterpene core or hydroxylated triterpenes core;wherein the core compound was dissolved in pyridine; wherein the acylchloride including Tigloyl chloride, angeloyl chloride, Acetyl chloride,Crotonoyl chloride, 3,3-Dimethylartyloyl chloride, senecioyl chloride,Cinnamoyl chloride, Pentenoyl chloride, Hexanoyl chloride, benzoylchloride, Ethylbutyryl chloride, Propionyl chloride, 2-Propenoylchloride, Isobutyryl chloride, Butyryl chloride, (2E)-2-pentenoylchloride, 4-Pentenoyl chloride, 5-Hexenoyl chloride, Heptanoyl chloride,Octanoyl chloride, Nonanoyl chloride, Decanoyl chloride, Lauroylchloride, Myristoyl chloride, and Oleoyl chloride; wherein the reactiontime for the mixture is stirred for 5 sec, 10 sec, 20 sec, 30 sec, 40sec, 1 min, 2 min, 5 min, 10 min, 30 min, 1 hr, 2 hr, 18 hr, 2 days or 3days; wherein the temperature is 0 C, 25 C, 50 C or 75 C temperature;wherein the acid including HCl or the base including NaHCO3 is added tothe reaction mixture; wherein the solution is then extracted 3 timeswith ethyl acetate and lyophilization; wherein the reaction product isdissolved in 80% acetonitrile—0.005% Trifluoroacetic acid or DMSO;wherein selecting the HPLC fractions for isolation is according to thecytotoxic activity of the reaction product obtained at a reaction timeof 5 sec, 10 sec, 20 sec, 30 sec, 40 sec, 1 min, 2 min, 5 min, 10 min,30 min, 1 hr, 2 hr, 18 hr, 2 days or 3 days. In an embodiment, thereaction time may be ove 3 days. In an embodiment, the experiment may beperformed under 0 C. In an embodiment, the experiment may be performedover 750 C. In embodiment, the attachment of sugar moiety(ies) can bebiosynthesized.

This invention provide the compound having structures of T1, T2, T3, T4,T5, T6, T7, T8, T9, T10, T11, T12, T13, T14, T15, T16, T17; T18, T19,T20, T21, T22, T23, T24, T25, T26, T27 wherein the R1, R2, R3, R4, R5,R8, R9, R10, R11, R12, R13, R14, R15, R16, R17, R18 are independentlyselected from the group of hydrogen, hydroxyl, methyl, O, O-angeloyl,O-tigloyl, O-senecioyl, O-acetyl, O-Crotonoyl, O-3,3-Dimethylartyloyl,O-Cinnamoyl, O-Pentenoyl, O-Hexanoyl, O-benzoyl, O-Ethylbutyryl,O-alkyl, O-dibenzoyl, O-benzoyl, O-alkanoyl, O-alkenoyl, O-benzoyl alkylsubstituted O-alkanoyl, O-alkanoyl substituted phenyl, O-alkenoylsubstituted phenyl, O-aryl, O-acyl, O-heterocylic, O-heteroraryl,O-alkenylcarbonyl, O-ethanoyl, O-propanoyl, O-propenoyl, O-butanoyl,O-butenoyl, O-pentanoyl, O-hexenoyl, O-heptanoyl, O-heptenoyl,O-octanoyl, O-octenoyl, O-nonanoyl, O-nonenoyl, O-decanoyl, O-decenoyl,O-propionyl, O-2-propenoyl, O-2-butenoyl, O-Isobutyryl,O-2-methylpropanoyl, O-2-ethylbutyryl, O-ethylbutanoyl,O-2-ethylbutanoyl, O-butyryl, O-(E)-2,3-Dimethylacryloyl,O-(E)-2-Methylcrotonoyl, O-3-cis-Methyl-methacryloyl,O-3-Methyl-2-butenoyl, O-3-Methylcrotonoyl, O-4-Pentenoyl,O-(2E)-2-pentenoyl, O-Caproyl, O-5-Hexenoyl, O-Capryloyl, O-Lauroyl,O-Dodecanoyl, O-Myristoyl, O-Tetradecanoyl, O-Oleoyl, O—C(2-18) Acyl,O-4-(dimethylamino)-2-methylbut-2-enoyl,O-4-[(2-methoxyethyl)amino]-2-methyl-4oxobut-2-enoyl, O-sugarmoiety(ies), O-acid moiety(ies), CH2O-angeloyl, CH2O-tigloyl,CH2O-senecioyl, CH2O-acetyl, CH2O-Crotonoyl, CH2O-3,3-Dimethylartyloyl,CH2O-Cinnamoyl, CH2O-Pentenoyl, CH2O-Hexanoyl, CH2O-benzoyl,CH2O-Ethylbutyryl, CH3, CH2OH, CH2O-alkyl, CH2O-dibenzoyl, CH2O-benzoyl,CH2O-alkanoyl, CH2O-alkenoyl, CH2O-benzoyl alkyl substituted O-alkanoyl,CH2O-alkanoyl substituted phenyl, CH2O-alkenoyl substituted phenyl,CH2O-aryl, CH2O-acyl, CH2O-heterocylic, CH2O-heteroraryl,CH2O-alkenylcarbonyl, CH2O-ethanoyl, CH2O-propanoyl, CH2O-propenoyl,CH2O-butanoyl, CH2O-butenoyl, CH2O-pentanoyl, CH2O-hexenoyl,CH2O-heptanoyl, CH2O-heptenoyl, CH2O-octanoyl, CH2O-octenoyl,CH2O-nonanoyl, CH2O-nonenoyl, CH2O-decanoyl, CH2O-decenoyl,CH2O-propionyl, CH2O-2-propenoyl, CH2O-2-butenoyl, CH2O-Isobutyryl,CH2O-2-methylpropanoyl, CH2O-2-ethylbutyryl, CH2O-ethylbutanoyl,CH2O-2-ethylbutanoyl, CH2O-butyryl, CH2O-(E)-2,3-Dimethylacryloyl,CH2O-(E)-2-Methylcrotonoyl, CH2O-3-cis-Methyl-methacryloyl,CH2O-3-Methyl-2-butenoyl, CH2O-3-Methylcrotonoyl, CH2O-4-Pentenoyl,CH2O-(2E)-2-pentenoyl, CH2O-Caproyl, CH2O-5-Hexenoyl, CH2O-Capryloyl,CH2O-Lauroyl, CH2O-Dodecanoyl, CH2O-Myristoyl, CH2O-Tetradecanoyl,CH2O-Oleoyl, CH2O—C(2-18) Acyl,CH2O-4-(dimethylamino)-2-methylbut-2-enoyl,CH2O-4-[(2-methoxyethyl)amino]-2-methyl-4oxobut-2-enoyl, alkane, alkeneand derivatives thereof. In an embodiment, the compound is attached asugar moiety(ies), acid moiety(ies) or alduronic acid, wherein the sugarmoiety(ies) is/are selected from a group of glucose, galactose,rhamnose, arabinose, xylose, fucose, allose, altrose, gulose, idose,lyxose, mannose, psicose, ribose, sorbose, tagatose, talose, fructose,alduronic acid, glucuronic acid, galacturonic acid, and derivatives orcombinations thereof. In an embodiment, the compound is attached a groupselected from (CnH2n)O-angeloyl, (CnH2n)O-tigloyl, (CnH2n)O-senecioyl,(CnH2n)O-acetyl, (CnH2n)O-Crotonoyl, (CnH2n)O-3,3-Dimethylartyloyl,(CnH2n)O-Cinnamoyl, (CnH2n)O-Pentenoyl, (CnH2n)O-Hexanoyl,(CnH2n)O-benzoyl, (CnH2n)O-Ethylbutyryl, (CnH2n)O-alkyl,(CnH2n)O-dibenzoyl, (CnH2n)O-benzoyl, (CnH2n)O-alkanoyl,(CnH2n)O-alkenoyl, (CnH2n)O-benzoyl alkyl substituted O-alkanoyl,(CnH2n)O-alkanoyl substituted phenyl, (CnH2n)O-alkenoyl substitutedphenyl, (CnH2n)O-aryl, (CnH2n)O-acyl, (CnH2n)O-heterocylic,(CnH2n)O-heteroraryl, (CnH2n)O-alkenylcarbonyl, (CnH2n)O-alkane,(CnH2n)O-alkene and (CnH2n)O-sugar moiety and (CnH2n)O-acid moiety;wherein the sugar moiety(ies) is/are included but not limited to a groupof glucose, galactose, rhamnose, arabinose, xylose, fucose, allose,altrose, gulose, idose, lyxose, mannose, psicose, ribose, sorbose,tagatose, talose, fructose, alduronic acid, glucuronic acid,galacturonic acid, and derivatives or combinations thereof; wherein n is1 or 2 or 3 or 4 or over 5.

This invention provide the compound having structures of T1, T2, T3, T4,T5, T6, T7, T8, T9, T10, T11, T12, T13, T14, T15, T16, T17; T18, T19,T20, T21, T22, T23, T24, T25. T26 provided for treating cancers,inhibition of cancer growth, cancer invasion, cells invasion, cancercell invasion; cell adhesion, cell attachment, cell circulating; fortreating mad cow disease; treating prion diseases; for treatingdiabetes; for inhibiting viruses; for preventing cerebral aging; forimproving memory; improving cerebral functions; for curing enuresis,frequent micturition, urinary incontinence; dementia, Alzheimer'sdisease, autism, brain trauma, Parkinson's disease or other diseasescaused by cerebral dysfunctions or neurodegeneration; for treatingarthritis, rheumatism, poor circulation, arteriosclerosis, Raynaud'ssyndrome, angina pectoris, cardiac disorder, coronary heart disease,headache, dizziness, kidney disorder; cerebrovascular diseasea;inhibiting NF-kappa B activation; for treating brain edema, severe acuterespiratory syndrome, respiratory viral diseases, chronic venousinsufficiency, hypertension, chronic venous disease, oedema,inflammation, hemorrhoids, peripheral edema formation, varicose veindisease, flu, post traumatic edema and postoperative swelling; forinhibiting blood clots, for inhibiting ethanol absorption; for loweringblood sugar; for regulating adrenocorticotropin and corticosteronelevels. This invention provides a composition for Anti-MS,anti-aneurysm, anti-asthmatic, anti-oedematous, anti-inflammatory,anti-bradykinic, anti-capillarihemorrhagic, anti-cephalagic,anti-cervicobrachialgic, anti-eclamptic, anti-edemic, anti-encaphalitic,anti-epiglottitic, anti-exudative, anti-flu, anti-fracture,anti-gingivitic, anti-hematomic, anti-herpetic, anti-histaminic,anti-hydrathritic, anti-meningitic, antioxidant, anti-periodontic,anti-phlebitic, anti-pleuritic, anti-raucedo, anti-rhinitic,anti-tonsilitic, anti-ulcer, anti-varicose, anti-vertiginous,cancerostatic, corticosterogenic, diuretic, fungicide, hemolytic,hyaluronidase inhibitor, lymphagogue, natriuretic, pesticide, pituitarystimulant, thymolytic, vasoprotective, inhibiting leishmaniases,modulating adhesion or angiogenesis of cells, anti-parasitic; increasethe expression of the genes: ANGPT2, DDIT3, LIF and NFKB1Z, andmanufacturing an adjuvant composition and venotonic treatment. Thecompound blocks the DNA synthesis of cancer cell; wherein the cancerscomprise breast cancer, leukocytic cancer, liver cancer, ovarian cancer,bladder cancer, prostatic cancer, skin cancer, bone cancer, braincancer, leukemia cancer, lung cancer, colon cancer, CNS cancer, melanomacancer, renal cancer, cervical cancer, esophageal cancer, testicularcancer, spleenic cancer, kidney cancer, lymphhatic cancer, pancreaticcancer, stomach cancer and thyroid cancer. This invention providescompounds to improve blood circulation; soothing stroke; Prevent plaqueformation and promote their dissipated; improve blood viscosity; reducecardiovascular; reduce cerebrovascular; reduce thrombosis,arteriosclerosis, coronary heart disease, hypertension, diabetes,thrombocytopenia purpura, hemoptysis, hematemesis; treating blood in thestool, uterine bleeding, traumatic bleeding, abdominal irritation,swelling, flutter, Blood circulation, swelling, pain; Treatingbronchiectasis, tuberculosis and lung abscess caused by too hemoptysis;reducing bleeding, antitussive, expectorant and analgesic effect, dilateblood vessels; reducing blood pressure and the treatment of cerebralarteriosclerosis; elevated blood lipids and reduced cholesterol.

Liposome is artificially prepared vesicles which made up of a lipidbilayer. Certain sizes of liposome can enter tumour sites from blood dueto the enhanced permeability and retention effect. While human bloodvessels are all surrounded by endothelial cells bound by tightjunctions, those tight junctions binding tumour vessels are leakier thanthose binding other vessels and thus liposomes are able to enter thesevessels to enhance the delivery, efficacy, bioavailability andabsorption of liposome enclosed drug. This invention provides methods touse liposomes or nanoparticles capsules as a carrier delivering thecompound as medicament, wherein the size of liposomes or nanoparticlescapsules is less than 200 nm or 100-200 nm or 50-100 nm or 5-50 nm orless than 50 nm, wherein the medicament is included but not limited fortreating cancer, inhibiting cancer growth, inhibiting cancer invasion,inhibiting cancer metastasis, modulating cell adhesion, modulating cellattachment, wherein the compound is selected from formula (2A) orformula (K) at the above.

Substitution, deletion and/or addition of any group in theabove-described compounds by other group(s) will be apparent to one ofordinary skill in the art based on the teachings of this application. Ina further embodiment, the substitution, deletion and/or addition of thegroup(s) in the compound of the invention does not substantially affectthe biological function of the compound is included in the invention.

In an embodiment, the compound is selected from the structures:

In embodiment, sugar moiety(ies) or acid moiety(ies) can be attached tothe above compounds. In embodiment, the attachment of sugar moiety(ies)can be biosynthesized; In embodiment, the attachment of acid moiety canbe biosynthesized; wherein the sugar moiety(ies) or acid moiety(ies)is/are included but not limited to a group of glucose, galactose,rhamnose, arabinose, xylose, fucose, allose, altrose, gulose, idose,lyxose, mannose, psicose, ribose, sorbose, tagatose, talose, fructose,alduronic acid, glucuronic acid, galacturonic acid, and derivatives orcombinations thereof;

This invention provides compounds by esterification of core compound (C)or (D1) with acetyl chloride, angeloyl chloride, tigloyl chloride,senecioyl chloride, Crotonoyl chloride, O-3,3-Dimethylartyloyl chloride,Cinnamoyl chloride, Pentenoyl chloride, Hexanoyl chloride, benzoylchloride, Ethylbutyryl chloride, and isolation of the compounds withHPLC, for treating cancer, inhibiting cancer growth, inhibiting cancerinvasion, inhibiting cancer metastasis, modulating cell adhesion,modulating cell attachment, wherein the core compound selected from thefollowing:

Esterification of compounds (A), (C), or (D1) with acyl chlorideincluding Tigloyl chloride, angeloyl chloride, Acetyl chloride,Crotonoyl chloride, 3,3-Dimethylartyloyl chloride, senecioyl chloride,Cinnamoyl chloride, Pentenoyl chloride, Hexanoyl chloride, benzoylchloride, Ethylbutyryl chloride, ethanoyl chloride, propanoyl chloride,propenoyl chloride, butanoyl chloride, butenoyl chloride, pentanoylchloride, hexenoyl chloride, heptanoyl chloride, heptenoyl chloride,octanoyl chloride, octenoyl chloride, nonanoyl chloride, nonenoylchloride, decanoyl chloride, decenoyl chloride, propionyl chloride,2-propenoyl chloride, 2-butenoyl chloride, Isobutyryl chloride,2-methylpropanoyl chloride, 2-ethylbutyryl chloride, ethylbutanoylchloride, 2-ethylbutanoyl chloride, butyryl chloride,(E)-2,3-Dimethylacryloyl chloride, (E)-2-Methylcrotonoyl chloride,3-cis-Methyl-methacryloyl chloride, 3-Methyl-2-butenoyl chloride,3-Methylcrotonoyl chloride, 4-Pentenoyl chloride, (2E)-2-pentenoylchloride, Caproyl chloride, 5-Hexenoyl chloride, Capryloyl chloride,Lauroyl chloride, Dodecanoyl chloride, Myristoyl chloride, Tetradecanoylchloride, Oleoyl chloride, C (2-18) Acyl chloride,

The compounds vary in composition when the time or temperature of thereaction is changed. The peaks, fractions and compounds are selectedaccording to the activities of times studies and the changes of peaks.The compounds having strong to weak activities are selected andisolated. The anti cancer activities are the MTT studies of bone (U2OS),lung (H460), bladder (HTB-9), ovary (ES2), colon (HCT116), pancreas(Capan), ovary (OVCAR3), prostate (DU145), skin (SK-Mel-5), mouth (KB),kidney (A498), breast (MCF-7), liver (HepG2), brain (T98G), luekemia(K562), cervix (HeLa). The active esterification products are purifiedwith HPLC. The reaction product of mixtures and individual compounds aretested with MTT Cytotoxic Assay. Details of method are in Experiment 3of the present application. A second esterification of compound can beselected from the above experiment results to produce new activecompounds. A partial esterification compound is selected from the aboveexperiments to perform a second or repeated with a third esterificationwith different acyl chloride in order to produce new active compoundswith the experiments in the present application, wherein the compoundcan be selected from K, (H1) or (H2):

R1, R2, R3, R4, R5, R8, R9, R10, R11, R12, R13, R14, R15, R16, R17, R18are independently selected from the group of CH3, CH2OH, COOH, hydrogen,hydroxyl, methyl, O-angeloyl, O-tigloyl, O-senecioyl, O-acetyl,O-Crotonoyl, O-3,3-Dimethylartyloyl, O-Cinnamoyl, O-Pentenoyl,O-Hexanoyl, O-benzoyl, O-Ethylbutyryl, O-alkyl, O-dibenzoyl, O-benzoyl,O-alkanoyl, O-alkenoyl, O-benzoyl alkyl substituted O-alkanoyl,O-alkanoyl substituted phenyl, O-alkenoyl substituted phenyl, O-aryl,O-acyl, O-heterocylic, O-heteroraryl, O-alkenylcarbonyl, O-alkane,O-alkene, O-ethanoyl, O-propanoyl, O-propenoyl, O-butanoyl, O-butenoyl,O-pentanoyl, O-hexenoyl, O-heptanoyl, O-heptenoyl, O-octanoyl,O-octenoyl, O-nonanoyl, O-nonenoyl, O-decanoyl, O-decenoyl, O-propionyl,O-2-propenoyl, O-2-butenoyl, O-Isobutyryl, O-2-methylpropanoyl,O-2-ethylbutyryl, O-ethylbutanoyl, O-2-ethylbutanoyl, O-butyryl,O-(E)-2,3-Dimethylacryloyl, O-(E)-2-Methylcrotonoyl,O-3-cis-Methyl-methacryloyl, O-3-Methyl-2-butenoyl, O-3-Methylcrotonoyl,O-4-Pentenoyl, O-(2E)-2-pentenoyl, O-Caproyl, O-5-Hexenoyl, O-Capryloyl,O-Lauroyl, O-Dodecanoyl, O-Myristoyl, O-Tetradecanoyl, O-Oleoyl,O—C(2-18) Acyl; CH2O-angeloyl, CH2O-tigloyl, CH2O-senecioyl,CH2O-acetyl, CH2O-Crotonoyl, CH2O-3,3-Dimethylartyloyl, CH2O-Cinnamoyl,CH2O-Pentenoyl, CH2O-Hexanoyl, CH2O-benzoyl, CH2O-Ethylbutyryl, CH3,CH2OH, CH2O-alkyl, CH2O-dibenzoyl, CH2O-benzoyl, CH2O-alkanoyl,CH2O-alkenoyl, CH2O-benzoyl alkyl substituted O-alkanoyl, CH2O-alkanoylsubstituted phenyl, CH2O-alkenoyl substituted phenyl, CH2O-aryl,CH2O-acyl, CH2O-heterocylic, CH2O-heteroraryl, CH2O-alkenylcarbonyl,CH2O-ethanoyl, CH2O-propanoyl, CH2O-propenoyl, CH2O-butanoyl,CH2O-butenoyl, CH2O-pentanoyl, CH2O-hexenoyl, CH2O-heptanoyl,CH2O-heptenoyl, CH2O-octanoyl, CH2O-octenoyl, CH2O-nonanoyl,CH2O-nonenoyl, CH2O-decanoyl, CH2O-decenoyl, CH2O-propionyl,CH2O-2-propenoyl, CH2O-2-butenoyl, CH2O-Isobutyryl,CH2O-2-methylpropanoyl, CH2O-2-ethylbutyryl, CH2O-ethylbutanoyl,CH2O-2-ethylbutanoyl, CH2O-butyryl, CH2O-(E)-2,3-Dimethylacryloyl,CH2O-(E)-2-Methylcrotonoyl, CH2O-3-cis-Methyl-methacryloyl,CH2O-3-Methyl-2-butenoyl, CH2O-3-Methylcrotonoyl, CH2O-4-Pentenoyl,CH2O-(2E)-2-pentenoyl, CH2O-Caproyl, CH2O-5-Hexenoyl, CH2O-Capryloyl,CH2O-Lauroyl, CH2O-Dodecanoyl, CH2O-Myristoyl, CH2O-Tetradecanoyl,CH2O-Oleoyl, CH2O—C(2-18) Acyl;CH2O-4-(dimethylamino)-2-methylbut-2-enoyl,CH2O-4-[(2-methoxyethyl)amino]-2-methyl-4oxobut-2-enoyl or wherein any 1or 2 or 3 or 4 of R1, R2, R3, R4, R5, R8, R10, R16, R17, R18 is/areindependently attached an O-angeloyl, O-tigloyl, O-senecioyl, O-acetyl,O-Crotonoyl, O-3,3-Dimethylartyloyl, O-Cinnamoyl, O-Pentenoyl,O-Hexanoyl, O-benzoyl, O-Ethylbutyryl, O-alkyl, O-dibenzoyl, O-benzoyl,O-alkanoyl, O-alkenoyl, O-benzoyl alkyl substituted O-alkanoyl,O-alkanoyl substituted phenyl, O-alkenoyl substituted phenyl, O-aryl,O-acyl, O-heterocylic, O-heteroraryl, O-alkenylcarbonyl, O-ethanoyl,O-propanoyl, O-propenoyl, O-butanoyl, O-butenoyl, O-pentanoyl,O-hexenoyl, O-heptanoyl, O-heptenoyl, O-octanoyl, O-octenoyl,O-nonanoyl, O-nonenoyl, O-decanoyl, O-decenoyl, O-propionyl,O-2-propenoyl, O-2-butenoyl, O-Isobutyryl, O-2-methylpropanoyl,O-2-ethylbutyryl, O-ethylbutanoyl, O-2-ethylbutanoyl, O-butyryl,O-(E)-2,3-Dimethylacryloyl, O-(E)-2-Methylcrotonoyl,O-3-cis-Methyl-methacryloyl, O-3-Methyl-2-butenoyl, O-3-Methylcrotonoyl,O-4-Pentenoyl, O-(2E)-2-pentenoyl, O-Caproyl, O-5-Hexenoyl, O-Capryloyl,O-Lauroyl, O-Dodecanoyl, O-Myristoyl, O-Tetradecanoyl, O-Oleoyl,O—C(2-18) Acyl, CH2O-angeloyl, CH2O-tigloyl, CH2O-senecioyl,CH2O-acetyl, CH2O-Crotonoyl, CH2O-3,3-Dimethylartyloyl, CH2O-Cinnamoyl,CH2O-Pentenoyl, CH2O-Hexanoyl, CH2O-benzoyl, CH2O-Ethylbutyryl, CH3,CH2OH, CH2O-alkyl, CH2O-dibenzoyl, CH2O-benzoyl, CH2O-alkanoyl,CH2O-alkenoyl, CH2O-benzoyl alkyl substituted O-alkanoyl, CH2O-alkanoylsubstituted phenyl, CH2O-alkenoyl substituted phenyl, CH2O-aryl,CH2O-acyl, CH2O-heterocylic, CH2O-heteroraryl, CH2O-alkenylcarbonyl,CH2O-ethanoyl, CH2O-propanoyl, CH2O-propenoyl, CH2O-butanoyl,CH2O-butenoyl, CH2O-pentanoyl, CH2O-hexenoyl, CH2O-heptanoyl,CH2O-heptenoyl, CH2O-octanoyl, CH2O-octenoyl, CH2O-nonanoyl,CH2O-nonenoyl, CH2O-decanoyl, CH2O-decenoyl, CH2O-propionyl,CH2O-2-propenoyl, CH2O-2-butenoyl, CH2O-Isobutyryl,CH2O-2-methylpropanoyl, CH2O-2-ethylbutyryl, CH2O-ethylbutanoyl,CH2O-2-ethylbutanoyl, CH2O-butyryl, CH2O-(E)-2,3-Dimethylacryloyl,CH2O-(E)-2-Methylcrotonoyl, CH2O-3-cis-Methyl-methacryloyl,CH2O-3-Methyl-2-butenoyl, CH2O-3-Methylcrotonoyl, CH2O-4-Pentenoyl,CH2O-(2E)-2-pentenoyl, CH2O-Caproyl, CH2O-5-Hexenoyl, CH2O-Capryloyl,CH2O-Lauroyl, CH2O-Dodecanoyl, CH2O-Myristoyl, CH2O-Tetradecanoyl,CH2O-Oleoyl, CH2O—C(2-18) Acyl,CH2O-4-(dimethylamino)-2-methylbut-2-enoyl,CH2O-4-[(2-methoxyethyl)amino]-2-methyl-4oxobut-2-enoyl; R9, R1, R12,R13, R14, R15 are independently attached a CH3; or wherein R10 isattached an O-angeloyl, O-tigloyl, O-senecioyl, O-acetyl, O-Crotonoyl,O-3,3-Dimethylartyloyl, O-Cinnamoyl, O-Pentenoyl, O-Hexanoyl, O-benzoyl,O-Ethylbutyryl, O-alkyl, O-dibenzoyl, O-benzoyl, O-alkanoyl, O-alkenoyl,O-benzoyl alkyl substituted O-alkanoyl, O-alkanoyl substituted phenyl,O-alkenoyl substituted phenyl, O-aryl, O-acyl, O-heterocylic,O-heteroraryl, O-alkenylcarbonyl, O-ethanoyl, O-propanoyl, O-propenoyl,O-butanoyl, O-butenoyl, O-pentanoyl, O-hexenoyl, O-heptanoyl,O-heptenoyl, O-octanoyl, O-octenoyl, O-nonanoyl, O-nonenoyl, O-decanoyl,O-decenoyl, O-propionyl, O-2-propenoyl, O-2-butenoyl, O-Isobutyryl,O-2-methylpropanoyl, O-2-ethylbutyryl, O-ethylbutanoyl,O-2-ethylbutanoyl, O-butyryl, O-(E)-2,3-Dimethylacryloyl,O-(E)-2-Methylcrotonoyl, O-3-cis-Methyl-methacryloyl,O-3-Methyl-2-butenoyl, O-3-Methylcrotonoyl, O-4-Pentenoyl,O-(2E)-2-pentenoyl, O-Caproyl, O-5-Hexenoyl, O-Capryloyl, O-Lauroyl,O-Dodecanoyl, O-Myristoyl, O-Tetradecanoyl, O-Oleoyl, O—C(2-18) Acyl,O-4-(dimethylamino)-2-methylbut-2-enoyl, andO-4-[(2-methoxyethyl)amino]-2-methyl-4oxobut-2-enoyl, CH2O-angeloyl,CH2O-tigloyl, CH2O-senecioyl, CH2O-acetyl, CH2O-Crotonoyl,CH2O-3,3-Dimethylartyloyl, CH2O-Cinnamoyl, CH2O-Pentenoyl,CH2O-Hexanoyl, CH2O-benzoyl, CH2O-Ethylbutyryl, CH3, CH2OH, CH2O-alkyl,CH2O-dibenzoyl, CH2O-benzoyl, CH2O-alkanoyl, CH2O-alkenoyl, CH2O-benzoylalkyl substituted O-alkanoyl, CH2O-alkanoyl substituted phenyl,CH2O-alkenoyl substituted phenyl, CH2O-aryl, CH2O-acyl,CH2O-heterocylic, CH2O-heteroraryl, CH2O-alkenylcarbonyl, CH2O-ethanoyl,CH2O-propanoyl, CH2O-propenoyl, CH2O-butanoyl, CH2O-butenoyl,CH2O-pentanoyl, CH2O-hexenoyl, CH2O-heptanoyl, CH2O-heptenoyl,CH2O-octanoyl, CH2O-octenoyl, CH2O-nonanoyl, CH2O-nonenoyl,CH2O-decanoyl, CH2O-decenoyl, CH2O-propionyl, CH2O-2-propenoyl,CH2O-2-butenoyl, CH2O-Isobutyryl, CH2O-2-methylpropanoyl,CH2O-2-ethylbutyryl, CH2O-ethylbutanoyl, CH2O-2-ethylbutanoyl,CH2O-butyryl, CH2O-(E)-2,3-Dimethylacryloyl, CH2O-(E)-2-Methylcrotonoyl,CH2O-3-cis-Methyl-methacryloyl, CH2O-3-Methyl-2-butenoyl,CH2O-3-Methylcrotonoyl, CH2O-4-Pentenoyl, CH2O-(2E)-2-pentenoyl,CH2O-Caproyl, CH2O-5-Hexenoyl, CH2O-Capryloyl, CH2O-Lauroyl,CH2O-Dodecanoyl, CH2O-Myristoyl, CH2O-Tetradecanoyl, CH2O-Oleoyl,CH2O—C(2-18) Acyl; CH2O-4-(dimethylamino)-2-methylbut-2-enoyl,CH2O-4-[(2-methoxyethyl)amino]-2-methyl-4oxobut-2-enoyl or wherein R4and R10 are independently attached an CH2O-angeloyl, CH2O-tigloyl,CH2O-senecioyl, CH2O-acetyl, CH2O-Crotonoyl, CH2O-3,3-Dimethylartyloyl,CH2O-Cinnamoyl, CH2O-Pentenoyl, CH2O-Hexanoyl, CH2O-benzoyl,CH2O-Ethylbutyryl, CH2OH, CH2O-alkyl, CH2O-dibenzoyl, CH2O-benzoyl,CH2O-alkanoyl, CH2O-alkenoyl, CH2O-benzoyl alkyl substituted O-alkanoyl,CH2O-alkanoyl substituted phenyl, CH2O-alkenoyl substituted phenyl,CH2O-aryl, CH2O-acyl, CH2O-heterocylic, CH2O-heteroraryl,CH2O-alkenylcarbonyl, CH2O-ethanoyl, CH2O-propanoyl, CH2O-propenoyl,CH2O-butanoyl, CH2O-butenoyl, CH2O-pentanoyl, CH2O-hexenoyl,CH2O-heptanoyl, CH2O-heptenoyl, CH2O-octanoyl, CH2O-octenoyl,CH2O-nonanoyl, CH2O-nonenoyl, CH2O-decanoyl, CH2O-decenoyl,CH2O-propionyl, CH2O-2-propenoyl, CH2O-2-butenoyl, CH2O-Isobutyryl,CH2O-2-methylpropanoyl, CH2O-2-ethylbutyryl, CH2O-ethylbutanoyl,CH2O-2-ethylbutanoyl, CH2O-butyryl, CH2O-(E)-2,3-Dimethylacryloyl,CH2O-(E)-2-Methylcrotonoyl, CH2O-3-cis-Methyl-methacryloyl,CH2O-3-Methyl-2-butenoyl, CH2O-3-Methylcrotonoyl, CH2O-4-Pentenoyl,CH2O-(2E)-2-pentenoyl, CH2O-Caproyl, CH2O-5-Hexenoyl, CH2O-Capryloyl,CH2O-Lauroyl, CH2O-Dodecanoyl, CH2O-Myristoyl, CH2O-Tetradecanoyl,CH2O-Oleoyl, CH2O—C(2-18) Acyl;CH2O-4-(dimethylamino)-2-methylbut-2-enoyl,CH2O-4-[(2-methoxyethyl)amino]-2-methyl-4oxobut-2-enoyl or wherein R17and R18 are independently attached an O-angeloyl, O-tigloyl,O-senecioyl, O-acetyl, O-Crotonoyl, O-3,3-Dimethylartyloyl, O-Cinnamoyl,O-Pentenoyl, O-Hexanoyl, O-benzoyl, O-Ethylbutyryl, O-alkyl,O-dibenzoyl, O-benzoyl, O-alkanoyl, O-alkenoyl, O-benzoyl alkylsubstituted O-alkanoyl, O-alkanoyl substituted phenyl, O-alkenoylsubstituted phenyl, O-aryl, O-acyl, O-heterocylic, O-heteroraryl,O-alkenylcarbonyl, O-ethanoyl, O-propanoyl, O-propenoyl, O-butanoyl,O-butenoyl, O-pentanoyl, O-hexenoyl, O-heptanoyl, O-heptenoyl,O-octanoyl, O-octenoyl, O-nonanoyl, O-nonenoyl, O-decanoyl, O-decenoyl,O-propionyl, O-2-propenoyl, O-2-butenoyl, O-Isobutyryl,O-2-methylpropanoyl, O-2-ethylbutyryl, O-ethylbutanoyl,O-2-ethylbutanoyl, O-butyryl, O-(E)-2,3-Dimethylacryloyl,O-(E)-2-Methylcrotonoyl, O-3-cis-Methyl-methacryloyl,O-3-Methyl-2-butenoyl, O-3-Methylcrotonoyl, O-4-Pentenoyl,O-(2E)-2-pentenoyl, O-Caproyl, O-5-Hexenoyl, O-Capryloyl, O-Lauroyl,O-Dodecanoyl, O-Myristoyl, O-Tetradecanoyl, O-Oleoyl, O—C(2-18) Acyl;wherein R3 is OH or H or absent; wherein R1, R2, R3, R5, R8 are OH or Hor absent; wherein R9, R11, R12, R13, R14, and R15 are CH3; or whereinR1, R2, R5, R8 represent OH; R3 represents OH, H or absent; R4, R10represent CH2Oangeloyl; R9, R11, R12, R13, R14, R15 represent CH3; orwherein R1, R2, R5, R8 represent OH or O-tigloyl; R3 represents OH, H,or absent; R4, R10 represent CH2O tigloyl; R9, R11, R12, R13, R14, R15represent CH3; wherein the group attaching to the core compound selectedfrom acetyl, angeloyl, tigloyl, senecioyl, Crotonoyl,O-3,3-Dimethylartyloyl, Cinnamoyl, Pentenoyl, Hexanoyl, benzoyl,Ethylbutyryl, alkyl, dibenzoyl, benzoyl, methylbutanoyl,methylpropanoyl, alkanoyl, alkenoyl, benzoyl alkyl substituted alkanoyl,alkanoyl substituted phenyl, alkenoyl substituted phenyl, aryl, acyl,heterocylic, heteroraryl, alkenylcarbonyl,O-4-(dimethylamino)-2-methylbut-2-enoyl, andO-4-[(2-methoxyethyl)amino]-2-methyl-4oxobut-2-enoyl are interchangeableor replaceable thereof. They can be the same group or in combinationthereof. The compounds of present application on cells is arrestingcells in the S-phase and blocking their entering into the G2/M phase ofcell cycle. The compounds block the DNA synthesis of cancer cell. Thisinvention provides compounds and method for improving blood circulation;soothing stroke; Prevent plaque formation and promote their dissipated;improve blood viscosity; reducing cardiovascular; reducingcerebrovascular; reducing thrombosis, arteriosclerosis, coronary heartdisease, hypertension, diabetes, thrombocytopenia purpura, hemoptysis,hematemesis; treating blood in the stool, uterine bleeding, traumaticbleeding, abdominal irritation, swelling, flutter, Blood circulation,swelling, pain; Treating bronchiectasis, tuberculosis and lung abscesscaused by too hemoptysis; reducing bleeding, antitussive, expectorantand analgesic effect, dilate blood vessels; reducing blood pressure andthe treatment of cerebral arteriosclerosis; elevated blood lipids andreduced cholesterol.

A composition comprising an effective amount of compound selected fromthe above formula or a salt, ester, metabolite or derivative thereof canbe used as a medicament for blocking the invasion, migration, metastasisof cancer cells, inhibiting tumor or cancer cell growth and for treatingcancer, wherein the cancers comprise breast cancer, leukocytic cancer,liver cancer, ovarian cancer, bladder cancer, prostatic cancer, skincancer, bone cancer, brain cancer, leukemia cancer, lung cancer, coloncancer, CNS cancer, melanoma cancer, renal cancer, cervical cancer,esophageal cancer, testicular cancer, spleenic cancer, kidney cancer,lymphhatic cancer, pancreatic cancer, stomach cancer and thyroid cancer.

This invention provides a composition comprising the compounds providedin the invention for treating cancers; for inhibiting viruses; forpreventing cerebral aging; for improving memory; improving cerebralfunctions; for curing enuresis, frequent micturition, urinaryincontinence; dementia, Alzheimer's disease, autism, brain trauma,Parkinson's disease or other diseases caused by cerebral dysfunctions;for treating arthritis, rheumatism, poor circulation, arteriosclerosis,Raynaud's syndrome, angina pectoris, cardiac disorder, coronary heartdisease, headache, dizziness, kidney disorder; diabetes; cerebrovasculardiseasea; inhibiting NF-kappa B activation; for treating brain edema,severe acute respiratory syndrome, respiratory viral diseases, chronicvenous insufficiency, hypertension, chronic venous disease, oedema,inflammation, hemonhoids, peripheral edema formation, varicose veindisease, flu, post traumatic edema and postoperative swelling; forinhibiting blood clots, for inhibiting ethanol absorption; for loweringblood sugar; for regulating adrenocorticotropin and corticosteronelevels. This invention provides a composition for Anti-MS,anti-aneurysm, anti-asthmatic, anti-oedematous, anti-inflammatory,anti-bradykinic, anti-capillarihemorrhagic, anti-cephalagic,anti-cervicobrachialgic, anti-eclamptic, anti-edemic, anti-encaphalitic,anti-epiglottitic, anti-exudative, anti-flu, anti-fracture,anti-gingivitic, anti-hematomic, anti-herpetic, anti-histaminic,anti-hydrathritic, anti-meningitic, antioxidant, anti-periodontic,anti-phlebitic, anti-pleuritic, anti-raucedo, anti-rhinitic,anti-tonsilitic, anti-ulcer, anti-varicose, anti-vertiginous,cancerostatic, corticosterogenic, diuretic, fungicide, hemolytic,hyaluronidase inhibitor, lymphagogue, natriuretic, pesticide, pituitarystimulant, thymolytic, vasoprotective, inhibiting leishmaniases,modulating adhesion or angiogenesis of cells, anti-parasitic; increasethe expression of the genes: ANGPT2, DDIT3, LIF and NFKB1Z, andmanufacturing an adjuvant composition and venotonic treatment. Thecomposition block the DNA synthesis of cancer cell.

Alkenyl means unsaturated linear or branched structures and combinationsthereof, having formula R2 C═CR2, one or more double bonds therein.Examples of alkenyl groups include vinyl, propenyl, isopropenyl,butenyl, s- and t-butenyl, pentenyl, hexenyl, butadienyl, pentadienyl,and hexadienyl. An aryl is a functional group of organic moleculederived from an aromatic compound such as benzene, a 6-14 memberedcarbocyclic aromatic ring system comprising 1-3 benzene rings. If two ormore aromatic rings are present, then the rings are fused together, sothat adjacent rings share a common bond. Examples include phenyl andnaphthyl. The aryl group may be substituted with one or more substitutesindependently selected from halogen, alkyl or alkoxy. Acyl is afunctional group which can be obtained from an organic acid by theremoval of the carboxyl. Acyl groups can be written using the generalformula —COR, where there is a double bond between the carbon andoxygen. The names of acyl groups typically end in -yl, such as formyl,acetyl, propionyl, butyryl and benzoyl. Benzoyl is one of the acyls,C₆H₅COR, obtained from benzoic acid by the removal of the carboxyl. Aheterocyclic compound is a compound containing a heterocyclic ring whichrefers to a non-aromatic ring having 1-4 heteroatoms, said ring beingisolated or fused to a second ring selected from 3- to 7-memberedalicyclic ring containing O-4 heteroatoms, aryl and heteroaryl, whereinheterocyclic compounds include pyrrolidinyl, pipyrazinyl, morpholinyl,trahydrofuranyl, imidazolinyl, thiomorpholinyl, and the like.Heterocyclyl groups are derived from heteroarenes by removal of ahydrogen atom from any ring atom. Alkanoyl is the general name for anorganic functional group RCO—, where R represents hydrogen or an alkylgroup. Examples of alkanoyls are acetyl, propionoyl, butyryl,isobutyryl, pentanoyl and hexanoyl. Alkenoyl is an alkenylcarbonyl inwhich the alkenyl is defined above. Examples are pentenoyl (tigloyl) andpentenoyl (angeloyl). Alkyl is a radical containing only carbon andhydrogen atoms arranged in a chain, branched, cyclic or bicyclicstructure or their combinations, having 1-18 carbon atoms. Examplesinclude but are not limited to methyl, ethyl, propyl isopropyl, butyl,s- and t-butyl, pentyl, hexyl, heptyl, octyl, nonyl, undecyl, dodecyl,tridecyl, tetradecyl, pentadecyl, cyclopropyl, cyclobutyl, cyclopentyland cyclohexyl. Benzoyl alkyl substituted alkanoyl refers to straight orbranched alkanoyl substituted with at least one benzoyl and at least onealkyl, wherein the benzoyl is attached to a straight or branched alkyl.An example of a benzoyl alkyl substituted alkanoyl is benzoyl methylisobutanoyl. A sugar moiety is a segment of molecule comprising one ormore sugars or derivatives thereof or alduronic acid thereof.

(Y)Y3, Y and Y3 represent the same compound. YM and (ACH-Y) representthe same compound. Connecting moiety is a substructure or a group ofatoms which connect the functional group to a core compound. Example:angeloyl group is connected by a sugar moiety to a triterpene core.

Acetyl=ethanoyl; Propionyl=methylpropanoyl; Crotonoyl=2-butenoyl;Isobutyryl=2-methylpropanoyl; 2-Ethylbutyryl=2-Ethylbutanoyl;Butyryl=n-Butyryl=butanoyl=C-4 Acyl;trans-2-Methyl-2-butenoyl=(E)-2,3-Dimethylacryloylchloride=(E)-2-Methylcrotonoyl=3-cis-Methyl-methacryloyl=Tigloyl;3,3-Dimethylacryloyl=3-Methyl-2-butenoyl=3-Methylcrotonoyl=Senecioyl;Propionyl chloride=methylpropanoyl; Hexanoyl=Caproyl;Heptanoyl=Enanthic=Oenanthic; Octanoyl=Capryloyl; Dodecanoyl=Lauroyl;Tetradecanoyl=Myristoyl; C (2-18)Acyl is an acyl group having 2 to 18carbons. ethanoyl is a C-2 Acyl, propanoyl is a C-3 Acyl, propenoyl is aC-3 Acyl, propionyl is a C-3 Acyl, butanoyl is a C-4 Acyl, butenoyl is aC-4 Acyl, crotonoyl is a C-4 Acyl, pentanoyl is a C-5 Acyl, pentenoyl isa C-5 Acyl, angeloyl is C-5 Acyl, tigloyl is C-5 Acyl, senecioyl is C-5Acyl, hexanoyl is a C-6 Acyl, hexenoyl is a C-6 Acyl, heptanoyl is a C-7Acyl, heptenoyl is a C-7 Acyl, octanoyl is a C-8 Acyl, octenoyl is a C-8Acyl, nonanoyl is a C-9 Acyl, nonenoyl is a C-9 Acyl, decanoyl is a C-10Acyl, decenoyl is a C-10 Acyl, lauroyl is a C-12 Acyl, dodecanoyl is aC-12 Acyl, myristoyl is a C-14 Acyl, oleoyl is a C-18 Acyl.

The building blocks used in the invention including triterpenes,hydroxylated triterpenes, acetyl, angeloyl, tigloyl, senecioyl,Crotonoyl, 3,3-Dimethylartyloyl, Cinnamoyl, Pentenoyl, Hexanoyl,benzoyl, Ethylbutyryl, alkyl, dibenzoyl, benzoyl, methylbutanoyl,methylpropanoyl, alkanoyl, alkenoyl, benzoyl alkyl substituted alkanoyl,alkanoyl substituted phenyl, alkenoyl substituted phenyl, aryl, acyl,heterocylic, heteroraryl, alkenylcarbonyl, ethanoyl, propanoyl,propenoyl, butanoyl, butenoyl, pentanoyl, hexenoyl, heptanoyl,heptenoyl, octanoyl, octenoyl, nonanoyl, nonenoyl, decanoyl, decenoyl,propionyl, 2-propenoyl, 2-butenoyl, Isobutyryl, 2-methylpropanoyl,2-ethylbutyryl, ethylbutanoyl, 2-ethylbutanoyl, butyryl,(E)-2,3-Dimethylacryloyl, (E)-2-Methylcrotonoyl,3-cis-Methyl-methacryloyl, 3-Methyl-2-butenoyl, 3-Methylcrotonoyl,4-Pentenoyl, (2E)-2-pentenoyl, Caproyl, 5-Hexenoyl, Capryloyl, Lauroyl,Dodecanoyl, Myristoyl, Tetradecanoyl and Oleoyl,4-(dimethylamino)-2-methylbut-2-enoyl,4-[(2-methoxyethyl)amino]-2-methyl-4oxobut-2-enoyl or halides thereof,or chloride. thereof.

Acryloyl chloride [Synonym: 2-propenoly chloride]; Propionyl chloride[Synonym: methylpropanoyl chloride]; Crotonoyl chloride [Synonym:2-butenoyl chloride]; Isobutyryl chloride [Synonym: 2-methylpropanoylchloride]; 2-Ethylbutyryl chloride [Synonym: 2-Ethylbutanoyl chloride];Butyryl chloride (Synonym: n-Butyryl chloride, butanoyl chloride, or C-4Acyl halide); trans-2-Methyl-2-butenoyl chloride [Synonym:(E)-2,3-Dimethylacryloyl chloride, (E)-2-Methylcrotonoyl chloride,3-cis-Methyl-methacryloyl chloride, Tigloyl chloride];3,3-Dimethylacryloyl chloride [Synonym: 3-Methyl-2-butenoyl chloride,3-Methylcrotonoyl chloride, Senecioyl chloride]; Hexanoyl chloride[Synonym: Caproyl chloride]; Heptanoyl chloride [Synonym: Enanthicchloride, Oenanthic chloride] Octanoyl chloride [Synonym: Capryloylchloride].

In the presented experiments, concentrations of drug that inhibit 15%cell-growth or less (i.e. 85% of control or above) as compared to theno-drug control (DMSO) are considered non-cytotoxic concentrations. Inan embodiment, the concentrations of drug that inhibit 10% cell-growthor less (i.e. 90% of control or above) as compared to the no-drugcontrol (DMSO) are considered non-cytotoxic concentrations. In anembodiment, the concentrations of drug that inhibit 5% cell-growth orless (i.e. 95% of control or above) as compared to the no-drug control(DMSO) are considered non-cytotoxic concentrations. In an embodiment,the concentrations of drug that inhibit 20% cell-growth or less (i.e.80% of control or above) as compared to the no-drug control (DMSO) areconsidered non-cytotoxic concentrations. In an embodiment, theconcentrations of drug that inhibit 25% cell-growth or less (i.e. 75% ofcontrol or above) as compared to the no-drug control (DMSO) areconsidered non-cytotoxic concentrations. In an embodiment, theconcentrations of drug that inhibit 30% cell-growth or less as comparedto the no-drug control (DMSO) are considered non-cytotoxicconcentrations. In an embodiment, the concentrations of drug thatinhibit 45% cell-growth or less as compared to the no-drug control(DMSO) are considered non-cytotoxic concentrations.

The triterpene compound or compounds selected from this invention can beadministered to a subject in need thereof, treating the subject, whereinincluding preventing cancer, or providing an adjuvant effect to thesubject, or inhibiting the initation or promotion of cancer, or killingthe cancer/tumor cells, or inhibiting cancer cell invasion. In anembodiment the compounds inhibit the activation of Nuclear Factor-kB,wherein inhibiting the localization or wherein binding the DNA. In anembodiment the compounds block the DNA synthesis. In an embodiment thecompounds induce apoptosis in cancer cells.

Determination of gene expression by Real-time PCR method (BrilliantQPCR, Agilent Technologies): The real-time polymerase chain reactionsfurther confirm the results obtained from microarray analysis. TheReal-time PCR results (shown below) confirmed that Compound Y3 and YMincrease the expression of the genes: ANGPT2, DDIT3, LIF and NFKB1Z,wherein the results in Table 19-21 disclosed in PCT/US09/34115, filedFeb. 13, 2009.

The saponins are partially hydrolyzed into a mixture of products whichcan be separated by HPLC. Specific partial hydrolysis of saponins canalso be achieved with enzymes. The glycosidases catalyze the hydrolysisof the glycosidic linkage. Galactosidase is an enzyme which catalyzesthe hydrolysis of galactosides. Glucosidase is an enzyme which breaksglucose from saponin. Other enzyme examples are xylanases, lactase,amylase, chitinase, sucrase, maltase, and neuraminidase.

The sugar moiety of the triterpenoid saponin (example Xanifolia Y) canbe removed by acid hydrolysis. The synthetic compound of ACH-Y isobtained. ACH-Y is a triterpene with acyl groups but no sugar moiety.The acyl group of the saponin (example Xanifolia Y) can be removed byalkaline hydrolysis. The synthetic compound AKOH-Y can be obtained.AKOH-Y is a pentacyclic triterpene with sugar moieties. A pentacyclictriterpene can be obtained by acid and alkaline hydroysis of saponinsfrom natural sources. A pentacyclic triterpene can be obtained bysynthetic methods (Reference: Surendra et al., Rapid andEnantioselective Synthetic Approches to Germanicol and Other PentacyclicTriterpenes, Journal of the American Chemical Society, 2008, 130(27),8865-8869). Pentacyclic triterpenes with sugar moieties can also beobtained by synthesis (Reference: Pie et al., Synthesis ofL-arabinopyranose containing hederagenin saponins, Tetrahedron 61 (2005)4347-4362). Acylation is the process of adding an acyl group to acompound. The Friedel-Crafts reaction is an example of this process. Anactive compound can be obtained by acylating a pentacyclic triterpenes,or hydroxylated triterpenes. In an embodiment, acylating C24, C28, C21and C22 of a pentacyclic triterpenes, or hydroxylated triterpenesproduce compounds for inhibiting cancer growth, cancer invasion, cellinvasion, cancer cell invasion, molecular cell invasion, cell attachmentadhesion, or cell circulation. In an embodiment, the acyl group(s) maybe at C3. In an embodiment, a sugar moiety is at C21, 22, or 28, whereinthe sugar moiety is attached with 2 acyl groups. In an embodiment,acylating the compounds of (A), (B), (C), (D1), (D2), (F), (G), (H),produce the compounds for inhibiting cancer invasion, cells invasion orcancer cell invasion; cancer metastasis; or cancer growth. The buildingblocks in the present application are used to synthesise activesaponins. In enbodiment, the sugar moiety(ies) is/are included but notlimited to a group of glucose, galactose, rhamnose, arabinose, xylose,fucose, allose, altrose, gulose, idose, lyxose, mannose, psicose,ribose, sorbose, tagatose, talose, fructose, alduronic acid, glucuronicacid, galacturonic acid, and derivatives or combinations thereof;

Acylating the compound (G) with angeloyl or tigloyl group gives thefollowing compounds

wherein R1, R2, R5, R8 represent OH or O-angeloyl; R3 represents OH, Hor O-angeloyl; R4, R10 represent CH3, CH2OH or CH2Oangeloyl; R3represents OH, H or O-angeloyl; R9, R11, R12, R13, R14, R15 representCH3; or wherein R1, R2, R5, R8 represent OH or O-tigloyl; R3 representsOH, H or O-tigloyl; R4, R10 represent CH3, CH2OH or CH2O tigloyl; R9,R11, R12, R13, R14, R15 represent CH3; wherein the compounds inhibitcancer growth, cancer invasion, cells invasion or cancer cell invasion.

Acylating the compound (G) with angeloyl, tigloyl, senecioyl, acetyl,Crotonoyl, 3,3-Dimethylartyloyl, Cinnamoyl, Pentenoyl, Hexanoyl,benzoyl, Ethylbutyryl, alkyl, dibenzoyl, benzoyl, alkanoyl, alkenoyl,benzoyl alkyl substituted O-alkanoyl, alkanoyl substituted phenyl,alkenoyl substituted phenyl, aryl, acyl, heterocylic, heteroraryl,CH2O-alkenylcarbonyl, alkane, alkene give the compound (K) wherein R1,R2, R5, R8 represent OH, O-angeloyl, O-tigloyl, O-senecioyl, O-acetyl,O-Crotonoyl, O-3,3-Dimethylartyloyl, O-Cinnamoyl, O-Pentenoyl,O-Hexanoyl, O-benzoyl, O-Ethylbutyryl, O-alkyl, O-dibenzoyl, O-benzoyl,O-alkanoyl, O-alkenoyl, O-benzoyl alkyl substituted O-alkanoyl,O-alkanoyl substituted phenyl, O-alkenoyl substituted phenyl, O-aryl,O-acyl, O-heterocylic, O-heteroraryl, O-alkenylcarbonyl; R4, R10represent CH3, CH2OH, CH2O-angeloyl, CH2O-tigloyl, CH2O-senecioyl,CH2O-acetyl, CH2O-Crotonoyl, CH2O-3,3-Dimethylartyloyl, CH2O-Cinnamoyl,CH2O-Pentenoyl, CH2O-Hexanoyl, CH2O-benzoyl, CH2O-Ethylbutyryl,CH2O-alkyl, CH2O-dibenzoyl, CH2O-benzoyl, CH2O-alkanoyl, CH2O-alkenoyl,CH2O-benzoyl alkyl substituted O-alkanoyl, CH2O-alkanoyl substitutedphenyl, CH2O-alkenoyl substituted phenyl, CH2O-aryl, CH2O-acyl,CH2O-heterocylic, CH2O-heteroraryl, CH2O-alkenylcarbonyl, alkane,alkene; R3 is absent of represents OH, H, O-angeloyl, O-tigloyl,O-senecioyl, O-acetyl, O-Crotonoyl, O-3,3-Dimethylartyloyl, O-Cinnamoyl,O-Pentenoyl, O-Hexanoyl, O-benzoyl, O-Ethylbutyryl, O-alkyl,O-dibenzoyl, O-benzoyl, O-alkanoyl, O-alkenoyl, O-benzoyl alkylsubstituted O-alkanoyl, O-alkanoyl substituted phenyl, O-alkenoylsubstituted phenyl, O-aryl, O-acyl, O-heterocylic, O-heteroraryl,O-alkenylcarbonyl; wherein R9, R11, R12, R13, R14, R15 represent CH3;wherein the compounds inhibit cancer growth, cancer invasion, cellsinvasion or cancer cell invasion; wherein the compound for use asmediator or inhibitor of adhesion protein or angiopoietin; wherein thecompounds use as mediator modulating the secretion, expression, orsynthesis of adhesion protein comprises reducing the fibronectin forinhibiting cell attachment, cell adhesion or cell circulation; whereinthe adhesion proteins comprise fibronectin, integrins family, myosin,vitronectin, collagen, laminin, polyglycans, cadherin, heparin,tenascin, CD₅₄, and CAM; the compounds use for anti adhesion therapy andtargeting adhesion molecules for therapy.

Applicant further states that anti-adhesion therapy and targetingadhesion molecules for therapy is a new direction for development ofdrugs. Some examples of anti-adhesion drugs in clinical trials areEfalizumab, Odulimomab, Alicaforsen, Aselizumab etc, which target variesadhesion proteins. Please see TEXT BOOK, Adhesion Molecules: Functionand Inhibition, (Reference 2), edited by Klaus Ley page 289-291, 297.

Adhesion molecules in inflammatory disease, (Reference 4), Abstract,line 7-8 “Blockade of the function of expression of CAM has emerged as anew therapeutic target in inflammatory diseases”. Applicants' inventionis an anti-adhesion therapy which is a new use of the compound as amediator or inhibitor of adhesion proteins and angiopoietins. Itinhibits excess adhesion and inhibits cell attachment.

In the present application, Applicants have used compounds selected fromstructure (2A) for anti adhesion therapy, as a mediator or inhibitor ofadhesion proteins and angiopoietins, and modulation of the cellattachment, and cell adhesion.

This invention provide a synthetic method to obtain semi-naturalcompounds by chemically removing functional groups of complex naturalproducts to the basic core structure before de-novo chemically adding onactive groups directly or sequentially by reaction with the active groupdonating chemical under different reaction temperature and time toproduce series of different active group modified core structurecompounds that can be fractionated and easily structurally determined aswell as screening for different bio-active efficacies and toxicities aspotential new drug candidates.

The activities of compounds are tested with cancer of leukemia(CCRF-CEM, HL60(TB), K-562, MOLT-4, RPMI8226, SR), lung (A549/ATCC,HOP-62, HOP92, NCI-H226, NCI-H322M, NCI-H460, colon (COL0205, HCC-2998,HCT-116, HCT-15, HT29, KM12, SW-620), CNS(SF-268, SF295, SF539, SNB-19,SNB-75, US51), melanoma (LOX IMVI, MALME-3M, M14, MDA-MB-3M, M14,MDA-MB-435, SK-MEL-2, SK-MEL-28, SK-MEL-5, UACC-257, UACC-62), ovary(ICTOV1, OVCAR-3, OVCAR-4, OVCAR-5, OVCAR-8, NCIADR-RES, SKOV3), renal(786-0, A498, ACHN, CAKI-1, SN12 C, TK-10, UO-31), prostate (PC-3,DU-145), breast (MCF7, MDA-MB-231, HS578T, T47D, MDA-MB-468). The roomtemperature is 25 C in the present application.

Experimental Details

Experiment details of herb extraction, analysis of extract components byHPLC, determination of the cell-growth activity effected by Xanifolia Ywith cells derived from different human organs using MTT Assay,purification of the bioactive components from plant extract,fractionation of plant extracts with FPLC, isolation of component Yswith preparative HPLC, determination of the chemical structure, cellexperiments and animal studying are disclosed in PCT/US05/31900, U.S.Ser. No. 11/289,142, U.S. Ser. No. 10/906,303, U.S. Ser. No. 11/131,551and U.S. Ser. No. 11/683,198, filed on Mar. 7, 2007, PCT/US2007/077273,filed Aug. 30, 2007, U.S. Ser. No. 60/890,380, filed on Feb. 16, 2007,U.S. Nos. 60/947,705, filed on Jul. 3, 2007, PCT/US2008/002086,1188-ALA-PCT, filed Feb. 15, 2008, App'l No. PCT/US09/34115, filed Feb.13, 2009, Experiments 1-23 of PCT/US2008/002086, 1188-ALA-PCT, filedFeb. 15, 2008 are incorporated in this application.

Experiment 1: Removal of the Sugar Moiety from Saponin by AcidHydrolysis

15 mg saponin was dissolved in 1 ml of Methanol. 1 ml of 2N HCl was thenadded. The mixture was refluxed in 80 C water bath for 5 hours. Thesolution was then neutralized by adding 2 ml of 1N NaOH (to final pH4-6). The aglycone was then extracted with ethylacetate 3 ml×2. Theextracts were collected and pooled. Further isolation of aglycone(sugar-removed saponin) was achieved by HPLC with isocratic elution of80-100% acetonitrile.

Experiment 2: Removal of the Acyl Group by Alkaline Hydrolysis

Methods: 20 mg of saponin was dissolved in 0.5 ml of 1N NaOH. Thesolution was incubated in 80 C water bath for 4 hours. It was cooled toroom temperature before neutralized with 0.5 ml 1N HCl (adjust pH toabout 3). The mixture was extracted with 2 ml 1-butanol 3 times. Thebutanol fractions were collected and lyophilized. The hydrolyzed saponinwith further purified with HPLC in a C-18 column eluted with 25%acetonitrile.

Experiment 3: Adding the Acyl Group to Triterpene by Esterification

Method: 40 mg of triterpene core (fraction IV) was dissolved in 1 mlpyridine in a 50 ml tube. Reaction is started by adding 0.2 ml of acylchloride (Tigloyl chloride, angeloyl chloride, Acetyl chloride,Crotonoyl chloride, 3,3-Dimethylartyloyl chloride (senecioyl chloride),Cinnamoyl chloride, Pentenoyl chloride, Hexanoyl chloride, benzoylchloride or Ethylbutyryl chloride). The mixture is stirred for 5 sec, 1min, 2 min, 5 min, 10 min, 30 min, 1 hr, 2 hr, 18 hr, 2 days or 3 daysat 0 C, 25 C or 75 C temperature. At the end of reaction, 5 ml of 2N HClor 1M NaHCO3 is added to the reaction mixture. The solution is thenextracted 3 times with 10 ml of ethyl acetate which is then evaporatedunder vacuum and at 45 C and lyophilization. The reaction product isdissolved in 80% acetonitrile—0.005% Trifluoroacetic acid or DMSO; andwas separated with HPLC. Selecting the HPLC fractions for isolation isaccording to the cytotoxic activity of the reaction product obtained ata specific reaction time. The active esterification products arepurified with HPLC. The reaction product of mixtures and individualcompounds are tested with MTT cytotoxic assay. Structures are determinedwith NMR. See examples FIGS. 1-12 (U.S. Ser. No. 14/313,080)

Experiment 4: Preparation of E4A

-   -   1. Beta-Escin dissolved in 1M NaOH (20 mg/ml) was incubated at        70 C for 5 hours.    -   2. The hydrolyzed solution was neutralized with HCl and the        water was evaporated by lyophilization.    -   3. The product was dissolved in 50% methanol and 1N HCl. The        mixture was incubated at 70 C for 5 hours.    -   4. The solution was neutralized with NaOH.    -   5. The hydrolyzed product was extracted with ethylacetate, which        was subsequently removed by evaporation.    -   6. Further purification of the hydrolyzed product (E4A) was        archived with FPLC chromatography in a C18 column equilibrated        with 70% acetonitrile/TFA at the flow rate of 1 ml/min.

Experiment 5: Esterification of E4A with Tigloyl Chloride

-   -   1. 50 mg of E4A in 1 ml pyridine, stir gently in a 50 ml tube.        Esterification was carried out at 25 C by adding 200 ul Tigloyl        chloride.    -   2. Stir for 1 minute; then immediately add 5 ml of 2N HCl.    -   3. Stir for 1 hour and sit at room-Temp over night.    -   4. Extract the esterification products with 10 ml ethylacetate.    -   5. Evaporate the ethylacetate.    -   6. Dissolve the sample with 1 ml DMSO.    -   7. Fractionate the reaction products with HPLC.    -   8. Collect samples.

Experiment 6: Isolation of E4A-Tig Active Compounds with HPLC

-   -   1. Column: ZORBAX ODS 9.4×250 mm, 5 um    -   2. Solvents: A: 45% AN/TFA; B: 100% AN/TFA    -   3. Chromatography conditions: a) Elution: Solvent A to B in 80        min; then with solvent B for 40 min; b) flow rate: 1 ml/mim. c)        Monitor OD: at 207 nm;

Experiment 7: MTT Experiment

Cells. HTB-9 (bladder), HeLa-S3 (cervix), DU145 (prostate), H460 (lung),MCF-7 (breast), K562 (leukemia), HCT116 (colon), HepG2 (liver), U2OS(bone), T98G (brain), SK-MEL-5 (Skin) and OVCAR 3, ES2 (ovary), Pancreas(Capan), Mouth (KB), Kidney (A498).

MTT Assay. The procedure for MTT assay followed the method described byCarmichael et al. (1987) with modifications. The cells were seeded intoa 96-well plate at for 24 hours before drug-treatment. The cells werethen exposed to the drugs for 48, 72, or 96 hours. After thedrug-treatment, MTT (0.5 mg/mL) was added to cultures and incubated foran hour. The formazan (product of the reduction of tetrazolium by viablecells) formed and was dissolved with DMSO and the O.D. at 490 nm, andwas measured by an ELISA reader. The MTT level of the cells beforedrug-treatment was also measured (T0). The % cell-growth (% G) iscalculated as: % G=(TD−T0/TC−T0)×100(1), where TC or TD represents O.D.readings of control or drug-treated cells. When T0>TD, then thecytotoxicity (LC) expressed as % of the control is calculated as: %LC=(TD−T0/T0)×100(2).

MTT Assay is performed to intermediate and final products fromexperiments.

Experiment 8: Chemical Synthesis, Isolation and Characterization ofE4A-Tig-R

Chemical synthesis of E4A-Tig-R: 1. Preparation of E4A; 2.Esterification of E4A with

Tigloyl Chloride; 3. Isolation of E4A-Tig-R with HPLC

Cytotoxic activity determination: 1. MTT assay

Chemical structure determination: 1. NMR analysis; 2. Mass Spectrumanalysis

Compound E4A-Tig-R: 24,28-O-Tigloyl-3β,16α, 21β, 22α, 24β,28-hexahydroxyolean-12-ene

Experiment 9: Chemical Synthesis, Isolation and Characterization ofE4A-Tig-N

Chemical synthesis of E4A-Tig-N: 1. Preparation of E4A; 2.Esterification of E4A with Tigloyl Chloride; 3. Isolation of E4A-Tig-Nwith HPLC

Cytotoxic activity determination: 1. MTT assay

Chemical structure determination: 1. NMR analysis; 2. Mass Spectrumanalysis

Experiment 10: Chemical Synthesis, Isolation and Characterization ofE4A-Tig-Q

Chemical synthesis of E4A-Tig-Q: 1. Preparation of E4A; 2.Esterification of E4A with Tigloyl Chloride; 3. Isolation of E4A-Tig-Qwith HPLC

Cytotoxic activity determination: 1. MTT assay

Chemical structure determination: 1. NMR analysis; 2. Mass Spectrumanalysis

Experiment 11: Chemical Synthesis, Isolation and Characterization ofE4A-Tig-V

Chemical synthesis of E4A-Tig-V: 1. Preparation of E4A; 2.Esterification of E4A with Tigloyl Chloride; 3. Isolation of E4A-Tig-Vwith HPLC

Cytotoxic activity determination: 1. MTT assay

Chemical structure determination: 1. NMR analysis; 2. Mass Spectrumanalysis

Experiment 12

Chemical Synthesis, Isolation and Characterization of E4A-Tig-T

Chemical synthesis of E4A-Tig-T: 1. Preparation of E4A; 2.Esterification of E4A with Tigloyl Chloride; 3. Isolation of E4A-Tig-Twith HPLC

Cytotoxic activity determination: 1. MTT assay

Chemical structure determination: 1. NMR analysis; 2. Mass Spectrumanalysis

Experiment 13: Chemical Synthesis, Isolation and Characterization ofE4A-Tig-U

Chemical synthesis of E4A-Tig-U: 1. Preparation of E4A; 2.Esterification of E4A with Tigloyl Chloride; 3. Isolation of E4A-Tig-Uwith HPLC

Cytotoxic activity determination: 1. MTT assay

Chemical structure determination: 1. NMR analysis; 2. Mass Spectrumanalysis

Experiment 14: Chemical Synthesis, Isolation and Characterization ofE4A-Tig-S

Chemical synthesis of E4A-Tig-S: 1. Preparation of E4A; 2.Esterification of E4A with Tigloyl Chloride; 3. Isolation of E4A-Tig-Swith HPLC

Cytotoxic activity determination: 1. MTT assay

Chemical structure determination: 1. NMR analysis; 2. Mass Spectrumanalysis

Experiment 15

Using Method in Experiment 3, Esterification of E4A with Acetyl,angeloyl, tigloyl, senecioyl, Crotonoyl, Cinnamoyl, Pentenoyl gave thefollowing compounds:

Experiment 16: Esterification of E4A-Tig-N with Senecioyl Chloride

Chemical synthesis of E4A-Tig-Sen-1: 1. Esterification of E4A-Tig-N withSenecioyl Chloride; 3. Isolation of E4A-Tig-Sen-1 with HPLC

Cytotoxic activity determination: 1. MTT assay

Chemical structure determination: 1. NMR analysis; 2. Mass Spectrumanalysis

Experiment 17

Esterification of E4A-Tig-N with Angeloyl Chloride, Acetyl Chloride,Crotonoyl chloride, 3,3-Dimethylartyloyl chloride, senecioyl chloride,Cinnamoyl chloride, Pentenoyl chloride, Hexanoyl chloride, benzoylchloride or Ethylbutyryl chloride; Isolation with HPLC; Cytotoxicactivity determination; Chemical structure determination with the methodof Experiment 8, gave the following compounds:

Experiment 18: Inhibition of Cell Adhesion

Methods and Results. ES2 or Hey8A cells were plated in T25 flasks withmedium containing 5 ug/ml of compounds selected from structure (2A)including E4A-Tig-R, E4A-Tig-V, E4A-Tig-S, E4A-Tig-N, E4A-Tig-Q,E4A-Tig-T. Cultures were incubated for 5 hours. Attached cells wereremoved from flasks by trypsinization and the numbers/amounts werecounted. Compare to no drug controls, 80 b 4% of ES2 cells and 60±4% ofHey8A cells were found attached to flasks under this condition. At 5ug/ml of above compounds, over 90% of unattached cells are alive asdetermined by the trypan Blue exclusion assay and by their ability tore-attach to flasks when plating in medium without tested compounds.However, with 10 ug/ml tested compounds, less than 40% of cells attachedto flasks and many of them are dead cells. This experiment shows thattested compounds inhibit cells adhesion process.

Experiment 19: Fibronectin Secretion Experiment

Western blot is applied in this invention as a method to detect thespecific proteins in treated and untreated cells with compounds in thisinvention, wherein the cells are bladder, cervix, prostate, lung,breast, leukemia, colon, liver, bone, brain, Skin, ovary, Pancreas(Capan), Mouth (KB), Kidney.

Cells: targeted cells were grown in RPMI 1640 medium. 1.5 million cellswere seeded in a T25 flask and grown for 24 hours before drug-treatment.

Drug-treatment: Cells cultures were replaced with fresh RPMI mediumcontaining either 2.5 ul of DMSO (as control) [D]; or 10, 20, 30, 40, 80ug/ml of tested compounds.

After 24 hours, aliquot of culture medium was taken out for Fibronectindetermination (Western blot method).

Cell viability at 24 hours was determined by MTT assay. Cultures werereplaced with RPMI medium (5 ml) with MTT and incubated for an hour. Theformation of formazan was dissolved in 10 ml of DMSO and OD at 570 nmwas measured (MTT units).

Western Blot: Spent culture medium was mixed with SDS sample buffer,boiled for 3 minutes before loading to SDS gel. Samples were applied toa 6-10% SDS gel and electrophoresis was conducted with 100 volts for 2hours. Protein was transferred to a nitrocellulose membraneelectrophoretically. The nitrocellulose blot was incubated with thefirst antibody and second antibody (AP conjugated, Promega S3721). Theimmuno-bands were developed with BCIP/NBT color development system.

Determination of Western blot band intensity: The band-images of Westernblot were captured with a digital camera and the intensity of bands wasdetermined using “Image J” software.

Results show that compounds of E4A-Tig-R, E4A-Tig-V, E4A-Tig-S,E4A-Tig-N, E4A-Tig-Q, E4A-Tig-T inhibit fibronectin secretion from20-40%. in bladder, cervix, prostate, lung, breast, leukemia, colon,liver, bone, brain, Skin, ovary, Pancreas (Capan), Mouth (KB), Kidney.

Experiment 20: Esterification of E4A with Propionyl Chloride

Methods: 50 mg of E4A in 1 ml pyridine, stir gently in a 50 ml tube.Esterification was carried out at 25 C by adding 200 ul Propionylchloride, and immediately withdrawn 200 ul from the mixture and added to1 ml of 2N HCl. (ASAP sample). At 1, 2, 5, 10 and 60 minutes afterward;200 ul of reaction mixture was similarly withdrawn and add to 1 ml of 2NHCl. Mixtures were sit at room-Temp over night. Extract theesterification products with 2 ml ethylacetate. Evaporate theethylacetate. Dissolve the sample with DMSO (final concentration of 40mg/ml). Fractionate the reaction products with HPLC (C18 column, 1ml/min).

HPLC condition: Column: C18 (9.4×250 mm, 5 um); Solvents: 80%Acetonitrile—0.005% TFA; Gradient: isocratic; Flow-rate: 1 ml/min; O.D.:207 nm, AT=1024; Chart speed: 0.1 cm/min; Run time: 120 min; MTT assay(Cytotoxicity determination) condition: Cells: ES2 (ovarian cancer).Cell density: plate10K cells per well over night before addition ofdrug. Drug incubation time: 2 days.

Experiment 21: Esterification of E4A with Isobutyryl Chloride

Methods: 52 mg of E4A in 1 ml pyridine, stir gently in a 50 ml tube.Esterification was carried out at 25° C. by adding 200 ul of isobutyrylchloride. 2 minute later, 4 ml 2N HCl was added to the reaction mixture.Mixtures were kept at room-Temp over night. Extract the esterificationproducts with 5 ml ethyl acetate. Evaporate the ethyl acetate. Dissolvethe sample with DMSO (final concentration of 40 mg/ml). Fractionate thereaction products with HPLC (C18 column).

HPLC condition: Column: C18 (9.4×250 mm, 5 um); Solvents: 80%Acetonitrile—0.005% TFA; Gradient: isocratic; Flow-rate: 1 ml/min; O.D.:207 nm, AT=1024; Chart speed: 0.1 cm/min; Run time: 200 min.

MTT assay (Cytotoxicity determination) condition: Cells: ES2 (ovariancancer); Cell density: platel OK cells per well over night beforeaddition of drug; Drug incubation time: 2 days.

Experiment 22

Esterification of E4A with 3,3-Dimethylacryloly Chloride from Differenttimes of esterification reaction. Reaction products obtained from eachtime of reaction (5 sec, 1 min, 2 min, 5 min, and 10 min) werefractionated by HPLC. The profile is plotted according to HPLC elutiontime and optical density of fractions. Reaction was performed at Roomtemperature and 0 C. Fractionate the reaction products with HPLC (C18column). Cytotoxic activity is determined with MTT. Chemical structuredetermined with NMR.

Experiment 23

Esterification of E4A with Pentenoyl chloride—from different times ofesterification reaction. Reaction products obtained from each time ofreaction (5 sec, 1 min, 2 min, 5 min, and 10 min) were fractionated byHPLC. The profile is plotted according to HPLC elution time and opticaldensity of fractions. Reaction was performed at Room temperature.Fractionate the reaction products with HPLC (C18 column). Cytotoxicactivity is determined with MTT. Chemical structure determined with NMR.

Experiment 24

Esterification of E4A with Hexanoly chloride from different times ofesterification reaction. Reaction products obtained from each time ofreaction (5 sec, 1 min, 2 min, 5 min, and 10 min) were fractionated byHPLC. The profile is plotted according to HPLC elution time and opticaldensity of fractions. Reaction was performed at 0 C. Fractionate thereaction products with HPLC (C18 column). Cytotoxic activity isdetermined with MTT. Chemical structure determined with NMR.

Experiment 25

Esterification of E4A with Acetyl chloride (H) from different times ofesterification reaction. Reaction products obtained from each time ofreaction (1 min, 2 min, 5 min and 10 min) were fractionated by HPLC. Theprofile is plotted according to HPLC elution time and optical density offractions. Reaction was performed at Room temperature. Fractionate thereaction products with HPLC (C18 column). Cytotoxic activity isdetermined with MTT. Chemical structure determined with NMR.

Experiment 26

Esterification products of E4A with Crotonoyl chloride (I) fromdifferent times of esterification reaction. Reaction products obtainedfrom each time of reaction (5 sec, 1 min, 2 min, 5 min and 10 min) werefractionated by HPLC. The profile is plotted according to HPLC elutiontime and optical density of fractions. Reaction was performed at Roomtemperature. Fractionate the reaction products with HPLC (C18 column).Cytotoxic activity is determined with MTT. Chemical structure determinedwith NMR.

Experiment 27

Esterification products of E4A with Cinnamoyl chloride (J) fromdifferent times of esterification reaction. Reaction products obtainedfrom each time of reaction (1 min, 1 hour, 2 hours, 18 hours, 18 hours(heat)) were fractionated by HPLC. The profile is plotted according toHPLC elution time and optical density of fractions. Reaction wasperformed at Room temperature and 75 C. Fractionate the reactionproducts with HPLC (C18 column). Cytotoxic activity is determined withMTT. Chemical structure determined with NMR.

Experiment 28

Esterification products of E4A with pentenoyl, hexanoyl, benzoyl,ethylbutyryl, propionyl, 2-propenoyl, isobutyryl, butyryl,(2E)-2-pentenoyl, 4-Pentenoyl, 5-hexenoyl, heptanoyl, octanoyl,nonanoyl, decanoyl, Lauroyl, myristoyl, from different times ofesterification reaction. Reaction products obtained from each time ofreaction were fractionated by HPLC. The profile is plotted according toHPLC elution time and optical density of fractions. Fractionate thereaction products with HPLC (C18 column). Cytotoxic activity isdetermined with MTT. Chemical structure determined with NMR.

Experiment 29

Esterification products of E4A with propanoyl, propenoyl, butanoyl,butenoyl, pentanoyl, hexenoyl, heptanoyl, heptenoyl, octanoyl, octenoyl,nonanoyl, nonenoyl, decanoyl, decenoyl, propionyl, 2-propenoyl,2-butenoyl, Isobutyryl, 2-methylpropanoyl, 2-ethylbutyryl,ethylbutanoyl, 2-ethylbutanoyl, butyryl, (E)-2,3-Dimethylacryloyl,(E)-2-Methylcrotonoyl, 3-cis-Methyl-methacryloyl, 3-Methyl-2-butenoyl,3-Methylcrotonoyl, 4-Pentenoyl, (2E)-2-pentenoyl, Caproyl, 5-Hexenoyl,Capryloyl, Lauroyl, Dodecanoyl, Myristoyl, Tetradecanoyl, Oleoyl fromdifferent times of esterification reaction. Reaction products obtainedfrom each time of reaction were fractionated by HPLC. The profile isplotted according to HPLC elution time and optical density of fractions.Fractionate the reaction products with HPLC (C18 column). Cytotoxicactivity is determined with MTT. Chemical structure determined with NMR.

Experiment 30

A comparison of non-cancerous with cancer cells: Method: A. Cells

-   -   W138 is a Normal Lung fibroblast. Cells were grew in MEM medium        supplemented with 10% FCS, antibiotics and glutamine. 20K cells        were seeded per well (96-welled plate) for one day before        drug-treatment    -   ES2 is a Ovary Clear cells carcinoma. Cells were grew in        RPMI-1640 medium supplemented with 10% FCS, antibiotics and        glutamine. 1 OK cells were seeded per well (96-welled plate) for        one day before drug-treatment.

B. Drug treatment:

-   -   Drug: Tig-S. stored as 1000× stock solution in DMSO.    -   Drug concentration used: from 0.15-20 ug/ml.    -   Drug-treatment was carried out for 2 days (ES2) or 6 days        (W138). For the 6 days incubation, cells were fed with fresh        medium (with drug) on day 3 and 5.

C. At the end of the drug-treatment, cytotoxic test was performed withMTT assay.

Conclusion:

-   -   For comparison between cancer cells (ES2) and non-cancerous        cells (W138), it is found that:

-   1. the IC₅₀ for ES2 cells (0.3 ug/ml) is lower than those of W138    cells (1.5 ug/ml), and

-   2. The IC₁₀₀ for ES2 cells (0.15-0.3 ug/ml) is lower than those of    W138 cells (10 ug/ml)

-   3. With 10 ug/ml of Tig-S, about 90% of ES2 cells died while only    10% of W138 cells died after the drug-treatment.    -   Based on these studies, it is concluded that the fast growing        tumor cells (ES2) are more sensitive to Tig-S than the slow        growing normal cells (W138).

Experiment 31: Inhibition of Cell Cycling

-   -   1. Method: Cells: Human leukemia cells K562 cultured in RPM11640        medium.    -   2. Drug: compound from present application or Tig-S (1000× stock        solution in DMSO).    -   3. Start cells concentration: 500000/ml.    -   4. Cells were cultured with drug (O-20 ug/ml) for total of three        days.    -   5. Cells were harvested by centrifugation (136×g, 6 minutes);        fixed with 70% ethanol and kept in −20 C before staining.    -   6. Staining: Fixed cells were stained with Propidium        iodide/RNase A/0.1% Triton X-100 in PBS.    -   7. Flow Cytometry Analysis was performed in Baylor Core Facility        with a LSRII instrument.    -   8. Analysis: Single cell was gated and cell count—FL2-Area        histogram were plotted.    -   9. Cell distribution in different cell-cycle phases (G0/G1, S,        G2/M) was analyzed.        Reseults: Cells with no drug, or with 0.15 ug/ml and 0.3 ug/ml        of Tig-S, have a similar (same) cell distribution in the G0/G1,        S and G2/M phases of cell-cycle. With higher Tig-S        concentrations, starting from 0.6 ug/ml, the cells in G2/M phase        decrease. The decrease of G2/M cells correlated with higher drug        concentrations (up to 20 ug/ml). These results indicate that        drug-treated cells were arrested in the S-phase and unable to        enter into the G2/M phase of the cell cycle.

Conclusion: The drug-effect of Tig-S on human leukemia K562 cells isarresting cells in the S-phase and blocking their entering into the G2/Mphase of cell cycle. The Compound Tig-S block the DNA synthesis ofcancer cell.

Experiment 32: Inhibition of H460 Cells Growth with Tig-S

Methods:

A. Cells

-   -   H460 cells are derived from a Human Lung large cell carcinoma.        Cells were grew in RPMI 1640 medium supplemented with 10% FCS,        antibiotics and glutamine. 5000 cells were seeded in a well        (96-welled plate) for one day before drug-treatment

B. Drug treatment:

-   -   Drug: Tig-S (stored as a 1000× stock solution in DMSO) was used.    -   Drug concentration used: from 0.15-20 ug/ml.    -   Drug-treatment was carried out for 1, 2 and 4 days. For the 4        days incubation, cells were fed with fresh medium (with drug) on        day 2.

C. At the end of the drug-treatment, cytotoxic test was performed withMTT assay.

Results:

1. Tig-S inhibits H460 cells' growth with the IC50 of drug 3 ug/ml.

2. Minimum cells growth inhibition was observed beyond drug 5 ug/ml.

3. No dead cell was found at drug concentration in 20 ug/ml.

The results indicate that Tig-S inhibits the H460 cell's growth, but isnot killing cells at high concentrations. Therefore, Tig-S is aneffective drug for inhibition of cancer growth but has low toxicity.

Experiment 33: Inhibition of Human Leukemia K562 Cells by Tig-S

Method:

-   -   1. Cells: Human leukemia cells K562 in RPM11640 medium.    -   2. Drug: Tig-S (1000× stock solution in DMSO).    -   3. Start cells concentration: 50×10K per ml (500000/ml).    -   4. Cells were cultured with or without drug for total of four        days.    -   5. Cell number is doubled after 2 days of incubation. Fresh        medium (equal volume, with or without drug) was then added to        culture.    -   6. Cells were counted every day.

Conclusion:

Tig-S inhibits Leukemia K562 cells growth with IC50 about 0.6 ug/ml.

No grow (IC100) was observed beginning on day 2 at 2.5 ug/ml or higher.

Experiment 34: Apoptosis in K562 Cells Induced by Tig-S

-   -   1. Cells: Human leukemia cells K562 cultured in RPM11640 medium.    -   2. Drug: Tig-S (1000× stock solution in DMSO).    -   3. Start cells concentration: 500000/ml.    -   4. Cells were cultured with drug (0-20 ug/ml) for Two days.    -   5. Collect cells from culture (1-4 million) by centrifugation        (136×g for 5 min. remove supernatant).    -   6. Wash cells with 1 ml of cold PBS, collect cells by        centrifugation (136×g, 5 min, remove supernatant).    -   7. Re-suspend cells in 200 ul Binding buffer (10 mM HEPES, 140        mM NaCl, 2.5 mM CaCl2, pH 7.4).    -   8. Stained cells by adding 10 ul Annexin V (Alexa Fluor 488,        Invitrogen cat # A13201), and 2 ul Propidium iodide (1 mg/ml in        water) to cell suspension. Mix.    -   9. Sit at Room temperature for 15 min.    -   10. Wash cells with 1 ml binding buffer. Collect cells by        centrifugation.    -   11. Re-suspend cells with in 1 ml of binding buffer for analysis        by flow cytometry.    -   12. Flow Cytometry analysis was performed in Baylor Core        Facility with a LSRII instrument.    -   13. Analysis: Control cells stained with PI or Annexin V-488        (Annexin V-FITC) alone were served as references. About 50000        cells were analyzed for each sample.    -   14. Cell distribution in following groups: live, early        apoptosis, late-apoptosis, total apoptosis and dead cells were        determined.    -   15. The percentage of cells in these groups is presented in the        FIG. 19.    -   Results:

The background apoptosis level of K562 cells (no-drug control) is about10-15%.

After drug-treatment with Tig-S, the apoptotic cells populationincreased (from 15% to 27%) with increased drug concentration (from 2.5ug/ml to 20 ug/ml).

Similarly, the dead cell population was also increase with the drugconcentration.

Conclusion:

Tig-S induces cell-death by the apoptosis mechanism (not necrosismechanism).

Experiment 35: The Haemolytic Assay

Erythrocytes (RBC) were isolated from human blood (EDTA whole blood,collected randomly). 50 ul of the 10% RBC suspension (in PBS) was addedto 2 ml of sample solutions (concentration range from 0.1 ug/ml to 400ug/ml) in PBS. The mixture was vortexed briefly and sat for 60 min atroom temperature. The mixture was spun at 3K for 10 min and the relativeamounts of lysed hemoglobin in the supernatant were measured at 540 nm.The synthetic compounds of present application were tested with thismethod.

Experiment 36: Animals Experiments

Methods

-   -   Athymic Nu/Nu mice are divided into two groups (A, and B).    -   On day 0, mice of group A and B were transplanted        intra-peritoneally (i.p.) with ES2 (human ovarian cancer) cells.    -   On day 1 to 5, mice from A group received daily administration        of 127 solvent by i.p. route.    -   On days 1 to 5, mice from B group received daily drug        administration (tested drug Tig-S in 127 solvent) by i.p. route        at dose of 100 mg/kg, twice daily.

Result in FIG. 22

Experiment 37: Animals Experiments

Methods

-   -   Athymic Nu/Nu mice are divided into two groups (A, and B).    -   On day 0, mice of group A and B were transplanted        intra-peritoneally (i.p.) with ES2 (human ovarian cancer) cells.    -   On day 1 to 5, mice from A group received daily administration        of 127 solvent by i.p. route.    -   On days 1 to 5, mice from B group received daily drug        administration (tested drug Tig-R in 127 solvent) by i.p. route        at dose of 100 mg/kg, twice daily.    -   Result in FIG. 23

Experiment 38: Animals Experiments

Methods

-   -   Athymic Nu/Nu mice are divided into two groups (A, and B).    -   On day 0, mice of group A and B were transplanted        intra-peritoneally (i.p.) with ES2 (human ovarian cancer) cells.    -   On day 1 to 5, mice from A group received daily administration        of 127 solvent by i.p. route.    -   On days 1 to 10, mice from B group received daily drug        administration (tested drug Tig-V in 127 solvent) by i.p. route        at dose of 50 mg/kg, twice daily.    -   Result in FIG. 24(U.S. Ser. No. 14/313,080)

Experiment 39: Inhibition of Capan Cells Growth by Tig-S

A. Cells: Capan cells are derived from Human pancreas carcinoma(pancreas). Cells were grew in RPMI 1640 medium supplemented with 10%FCS, antibiotics and glutamine. 10000 cells were seeded in a well(96-welled plate) for one day before drug-treatment

B. Drug treatment:

-   -   Drug: Tig-S (stored as a 1000× stock solution in DMSO) was used.    -   Drug concentration used: from 0.15-20 ug/ml.    -   Drug-treatment was carried out for 3 days.

C. At the end of the drug-treatment, cytotoxic test was performed withMTT assay. The percentage of drug-treated cells' growth as compared tothose of the no drug control is determined. See FIG. 37.

Experiment 40

Using method in Experiment 3, esterification of E4A2Y with acetyl,angeloyl, tigloyl, senecioyl, Crotonoyl, Cinnamoyl, Pentenoyl,4-(dimethylamino)-2-methylbut-2-enoyl, and4-[(2-methoxyethyl)amino]-2-methyl-4oxobut-2-enoyl gave the followingcompounds:

wherein:

-   -   1) R1, R2, R3, R5, R8 are OH; R17, R18 are O-acetyl;    -   2) R1, R2, R3, R5, R8 are OH; R17, R18 are O-angeloyl    -   3) R1, R2, R3, R5, R8 are OH; R17, R18 are O-tigloyl    -   4) R1, R2, R3, R5, R8 are OH; R17, R18 are O-senecioyl    -   5) R1, R2, R3, R5, R8 are OH; R17, R18 are O-Crotonoyl    -   6) R18, R2, R3, R5, R8 are OH; R17, R18 are O-Cinnamoyl    -   7) R18, R2, R3, R5, R8 are OH; R17, R18 are O-Pentenoyl    -   8) R18, R2, R3, R5, R8 are OH; R17, R18 are        O-4-(dimethylamino)-2-methylbut-2-enoyl    -   9) R18, R2, R3, R5, R8 are OH; R17, R18 are        O-4-[(2-methoxyethyl)amino]-2-methyl-4oxobut-2-enoyl    -   10) R18, R2, R3, R5, R8 are OH; R17, R1 are O-acetyl;    -   11) R18, R2, R3, R5, R8 are OH; R17, R1 are O-angeloyl    -   12) R18, R2, R3, R5, R8 are OH; R17, R1 are O-tigloyl    -   13) R18, R2, R3, R5, R8 are OH; R17, R1 are O-senecioyl    -   14) R18, R2, R3, R5, R8 are OH; R17, R1 are O-Crotonoyl    -   15) R18, R2, R3, R5, R8 are OH; R17, R1 are O-Cinnamoyl    -   16) R18, R2, R3, R5, R8 are OH; R17, R1 are O-Pentenoyl    -   17) R18, R2, R3, R5, R8 are OH; R17, R1 are        O-4-(dimethylamino)-2-methylbut-2-enoyl    -   18) R18, R2, R3, R5, R8 are OH; R17, R1 are        O-4-[(2-methoxyethyl)amino]-2-methyl-4oxobut-2-enoyl    -   19) R2, R3, R5, R8 are OH; R1, R17, R18 are O-acetyl;    -   20) R2, R3, R5, R8 are OH; R1, R17, R18 are O-angeloyl    -   21) R2, R3, R5, R8 are OH; R1, R17, R18 are O-tigloyl    -   22) R2, R3, R5, R8 are OH; R1, R17, R18 are O-senecioyl    -   23) R2, R3, R5, R8 are OH; R1, R17, R18 are O-Crotonoyl    -   24) R2, R3, R5, R8 are OH; R1, R17, R18 are O-Cinnamoyl    -   25) R2, R3, R5, R8 are OH; R1, R17, R18 are O-Pentenoyl    -   26) R2, R3, R5, R8 are OH; R1, R17, R18 are        O-4-(dimethylamino)-2-methylbut-2-enoyl    -   27) R2, R3, R5, R8 are OH; R1, R17, R18 are        O-4-[(2-methoxyethyl)amino]-2-methyl-4oxobut-2-enoyl.

What is claimed is:
 1. A synthetic compound selected from the structure:

R1, R2, R3, R4, R5, R8, R9, R11, R12, R13, R14, R15, R16 areindependently selected from the group of H, OH, CH3, CH2OH, COOH,hydrogen, hydroxyl, methyl, O-angeloyl, O-tigloyl, O-senecioyl,O-acetyl, O-Crotonoyl, O-3,3-Dimethylacryloyl, O-Cinnamoyl, O-Pentenoyl,O-Hexanoyl, O-benzoyl, O-Ethylbutyryl, O-alkyl, O-dibenzoyl, O-benzoyl,O-alkanoyl, O-alkenoyl, O-benzoyl alkyl substituted O-alkanoyl,O-alkanoyl substituted phenyl, O-alkenoyl substituted phenyl, O-aryl,O-acyl, O-heterocylic, O-heteroraryl, O-alkenylcarbonyl, O-alkane,O-alkene, O-acid moiety, O-ethanoyl, O-propanoyl, O-propenoyl,O-butanoyl, O-butenoyl, O-pentanoyl, O-hexenoyl, O-heptanoyl,O-heptenoyl, O-octanoyl, O-octenoyl, O-nonanoyl, O-nonenoyl, O-decanoyl,O-decenoyl, O-propionyl, O-2-propenoyl, O-2-butenoyl, O-Isobutyryl,O-2-methylpropanoyl, O-2-ethylbutyryl, O-ethylbutanoyl,O-2-ethylbutanoyl, O-butyryl, O-(E)-2,3-Dimethylacryloyl,O-(E)-2-Methylcrotonoyl, O-3-cis-Methyl-methacryloyl,O-3-Methyl-2-butenoyl, O-3-Methylcrotonoyl, O-4-Pentenoyl,O-(2E)-2-pentenoyl, O-Caproyl, O-5-Hexenoyl, O-Capryloyl, O-Lauroyl,O-Dodecanoyl, O-Myristoyl, O-Tetradecanoyl, O-Oleoyl, O-C(2-18) Acyl,O-4-(dimethylamino)-2-methylbut-2-enoyl,O-4-[(2-methoxyethyl)amino]-2-methyl-4oxobut-2-enoyl, CH2O-angeloyl,CH2O-tigloyl, CH2O-senecioyl, CH2O-acetyl, CH2O-Crotonoyl,CH2O-3,3-Dimethylacryloyl, CH2O-Cinnamoyl, CH2O-Pentenoyl,CH2O-Hexanoyl, CH2O-benzoyl, CH2O-Ethylbutyryl, CH2O-alkyl,CH2O-dibenzoyl, CH2O-benzoyl, CH2O-alkanoyl, CH2O-alkenoyl, CH2O-benzoylalkyl substituted O-alkanoyl, CH2O-alkanoyl substituted phenyl,CH2O-alkenoyl substituted phenyl, CH2O-aryl, CH2O-acyl,CH2O-heterocylic, CH2O-heteroraryl, CH2O-alkenylcarbonyl, CH2O-alkane,CH2O-alkene, CH2O-angeloyl, CH2O-tigloyl, CH2O-senecioyl, CH2O-acetyl,CH2O-Crotonoyl, CH2O-3,3-Dimethylacryloyl, CH2O-Cinnamoyl,CH2O-Pentenoyl, CH2O-Hexanoyl, CH2O-benzoyl, CH2O-Ethylbutyryl, CH3,CH2OH, CH2O-alkyl, CH2O-dibenzoyl, CH2O-benzoyl, CH2O-alkanoyl,CH2O-alkenoyl, CH2O-benzoyl alkyl substituted O-alkanoyl, CH2O-alkanoylsubstituted phenyl, CH2O-alkenoyl substituted phenyl, CH2O-aryl,CH2O-acyl, CH2O-heterocylic, CH2O-heteroraryl, CH2O-alkenylcarbonyl,CH2O-ethanoyl, CH2O-propanoyl, CH2O-propenoyl, CH2O-butanoyl,CH2O-butenoyl, CH2O-pentanoyl, CH2O-hexenoyl, CH2O-heptanoyl,CH2O-heptenoyl, CH2O-octanoyl, CH2O-octenoyl, CH2O-nonanoyl,CH2O-nonenoyl, CH2O-decanoyl, CH2O-decenoyl, CH2O-propionyl,CH2O-2-propenoyl, CH2O-2-butenoyl, CH2O-Isobutyryl,CH2O-2-methylpropanoyl, CH2O-2-ethylbutyryl, CH2O-ethylbutanoyl,CH2O-2-ethylbutanoyl, CH2O-butyryl, CH2O-(E)-2,3-Dimethylacryloyl,CH2O-(E)-2-Methylcrotonoyl, CH2O-3-cis-Methyl-methacryloyl,CH2O-3-Methyl-2-butenoyl, CH2O-3-Methylcrotonoyl, CH2O-4-Pentenoyl,CH2O-(2E)-2-pentenoyl, CH2O-Caproyl, CH2O-5-Hexenoyl, CH2O-Capryloyl,CH2O-Lauroyl, CH2O-Dodecanoyl, CH2O-Myristoyl, CH2O-Tetradecanoyl,CH2O-Oleoyl, CH2O-C(2-18) Acyl,CH2O-4-(dimethylamino)-2-methylbut-2-enoyl,CH2O-4-[(2-methoxyethyl)amino]-2-methyl-4oxobut-2-enoyl; wherein R10 isattached an O-angeloyl, O-tigloyl, O-senecioyl, O-, O-Crotonoyl,O-3,3-Dimethylacryloyl, O-Cinnamoyl, O-Pentenoyl, O-Hexanoyl, O-benzoyl,O-Ethylbutyryl, O-dibenzoyl, O-benzoyl, O-alkanoyl, O-alkenoyl,O-benzoyl alkyl substituted O-alkanoyl, O-alkanoyl substituted phenyl,O-alkenoyl substituted phenyl, O-heterocylic, O-heteroraryl,O-alkenylcarbonyl, O-ethanoyl, O-propanoyl, O-propenoyl, O-butanoyl,O-butenoyl, O-pentanoyl, O-hexenoyl, O-heptanoyl, O-heptenoyl,O-octanoyl, O-octenoyl, O-nonanoyl, O-nonenoyl, O-decanoyl, O-decenoyl,O-propionyl, O-2-propenoyl, O-2-butenoyl, O-Isobutyryl,O-2-methylpropanoyl, O-2-ethylbutyryl, O-ethylbutanoyl,O-2-ethylbutanoyl, O-butyryl, O-(E)-2,3-Dimethylacryloyl,O-(E)-2-Methylcrotonoyl, O-3-cis-Methyl-methacryloyl,O-3-Methyl-2-butenoyl, O-3-Methylcrotonoyl, O-4-Pentenoyl,O-(2E)-2-pentenoyl, O-Caproyl, O-5-Hexenoyl, O-Capryloyl, O-Lauroyl,O-Dodecanoyl, O-Myristoyl, O-Tetradecanoyl, O-Oleoyl,O-4-(dimethylamino)-2-methylbut-2-enoyl,O-4-[(2-methoxyethyl)amino]-2-methyl-4oxobut-2-enoyl, CH2O-angeloyl,CH2O-tigloyl, CH2O-senecioyl, CH2O-acetyl, CH2O-Crotonoyl,CH2O-3,3-Dimethylacryloyl, CH2O-Cinnamoyl, CH2O-Pentenoyl,CH2O-Hexanoyl, CH2O-benzoyl, CH2O-Ethylbutyryl, CH2O-dibenzoyl,CH2O-benzoyl, CH2O-alkanoyl, CH2O-alkenoyl, CH2O-benzoyl alkylsubstituted O-alkanoyl, CH2O-alkanoyl substituted phenyl, CH2O-alkenoylsubstituted phenyl, CH2O-heterocylic, CH2O-heteroraryl,CH2O-alkenylcarbonyl, CH2O-ethanoyl, CH2O-propanoyl, CH2O-propenoyl,CH2O-butanoyl, CH2O-butenoyl, CH2O-pentanoyl, CH2O-hexenoyl,CH2O-heptanoyl, CH2O-heptenoyl, CH2O-octanoyl, CH2O-octenoyl,CH2O-nonanoyl, CH2O-nonenoyl, CH2O-decanoyl, CH2O-decenoyl,CH2O-propionyl, CH2O-2-propenoyl, CH2O-2-butenoyl, CH2O-Isobutyryl,CH2O-2-methylpropanoyl, CH2O-2-ethylbutyryl, CH2O-ethylbutanoyl,CH2O-2-ethylbutanoyl, CH2O-butyryl, CH2O-(E)-2,3-Dimethylacryloyl,CH2O-(E)-2-Methylcrotonoyl, CH2O-3-cis-Methyl-methacryloyl,CH2O-3-Methyl-2-butenoyl, CH2O-3-Methylcrotonoyl, CH2O-4-Pentenoyl,CH2O-(2E)-2-pentenoyl, CH2O-Caproyl, CH2O-5-Hexenoyl, CH2O-Capryloyl,CH2O-Lauroyl, CH2O-Dodecanoyl, CH2O-Myristoyl, CH2O-Tetradecanoyl,CH2O-Oleoyl, CH2O-4-(dimethylamino)-2-methylbut-2-enoyl,CH2O-4-[(2-methoxyethyl)amino]-2-methyl-4oxobut-2-enoyl.
 2. A syntheticcompound having the structure:

wherein R10 is selected from the group of CH2O-angeloyl, CH2O-tigloyl,CH2O-senecioyl, CH2O-crotonoyl, CH2O-3,3-Dimethylacryloyl,CH2O-cinnamoyl, CH2O-pentenoyl, CH2O-hexanoyl, CH2O-benzoyl,CH2O-Ethylbutyryl, CH2O-4-(dimethylamino)-2-methylbut-2-enoyl, andCH2O-4-[(2-methoxyethyl)amino]-2-methyl-4oxobut-2-enoyl, wherein R1, R2,R4, R5, and R8 are independently selected from the group of CH2OH,hydroxyl, O-angeloyl, O-tigloyl, O-senecioyl, O-acetyl, O-crotonoyl,O-3,3-Dimethylacryloyl, O-cinnamoyl, O-pentenoyl, O-hexanoyl, O-benzoyl,O-ethylbutyryl, O-4-(dimethylamino)-2-methylbut-2-enoyl,O-4-[(2-methoxyethyl)amino]-2-methyl-4oxobut-2-enoyl, CH2O-angeloyl,CH2O-tigloyl, CH2O-senecioyl, CH2O-acetyl, CH2O-crotonoyl,CH2O-3,3-Dimethylacryloyl, CH2O-cinnamoyl, CH2O-pentenoyl,CH2O-hexanoyl, CH2O-benzoyl, and CH2O-ethylbutyrylCH2O-4-(dimethylamino)-2-methylbut-2-enoyl,CH2O-4-[(2-methoxyethyl)amino]-2-methyl-4oxobut-2-enoyl, wherein R3 isOH or H; wherein R9, R11, R12, R13, R14, and R15 are CH3.
 3. Thecompound of claim 2, wherein R10 is CH2O-angeloyl, CH2O-tigloyl,CH2O-senecioyl, CH2O-crotonoyl, CH2O-3,3-Dimethylacryloyl,CH2O-cinnamoyl, CH2O-Pentenoyl, CH2O-Hexanoyl, CH2O-benzoyl, orCH2O-Ethylbutyryl CH2O-4-(dimethylamino)-2-methylbut-2-enoyl,CH2O-4-[(2-methoxyethyl)amino]-2-methyl-4oxobut-2-enoyl, and at least ofR1, R2, R4, R5, R8, are O-angeloyl, O-tigloyl, O-senecioyl, O-acetyl,O-crotonoyl, O-3,3-Dimethylacryloyl, O-cinnamoyl, O-pentenoyl,O-hexanoyl, O-benzoyl, O-ethylbutyryl,O-4-(dimethylamino)-2-methylbut-2-enoyl,O-4-[(2-methoxyethyl)amino]-2-methyl-4oxobut-2-enoyl, —CH2O-angeloyl,CH2O-tigloyl, CH2O-senecioyl, CH2O-crotonoyl, CH2O-3,3-Dimethylacryloyl,CH2O-cinnamoyl, CH2O-Pentenoyl, CH2O-Hexanoyl, CH2O-benzoyl, orCH2O-Ethylbutyryl or O-4-(dimethylamino)-2-methylbut-2-enoyl,O-4-[(2-methoxyethyl)amino]-2-methyl-4oxobut-2-enoyl.
 4. The compound ofclaim 2, wherein R1 is O-angeloyl, O-tigloyl, O-senecioyl, O-acetyl,O-crotonoyl, O-3,3-Dimethylacryloyl, O-cinnamoyl, O-pentenoyl,O-hexanoyl, O-benzoyl, or O-ethylbutyryl,O-4-(dimethylamino)-2-methylbut-2-enoyl,O-4-[(2-methoxyethyl)amino]-2-methyl-4oxobut-2-enoyl, and R10 isCH2O-angeloyl, CH2O-tigloyl, CH2O-senecioyl, CH2O-crotonoyl,CH2O-3,3-Dimethylacryloyl, CH2O-cinnamoyl, CH2O-pentenoyl,CH2O-hexanoyl, CH2O-benzoyl, CH2O-ethylbutyryl,CH2O-4-(dimethylamino)-2-methylbut-2-enoyl,CH4O-4-[(2-methoxyethyl)amino]-2-methyl-4oxobut-2-enoyl.
 5. The compoundof claim 2, wherein R10 is CH2O-angeloyl, CH2O-tigloyl, CH2O-senecioyl,CH2O-crotonoyl, CH2O-3,3-Dimethylacryloyl, CH2O-cinnamoyl,CH2O-Pentenoyl, CH2O-Hexanoyl, CH2O-benzoyl, CH2O-EthylbutyrylCH2O-4-(dimethylamino)-2-methylbut-2-enoyl,CH2O-4-[(2-methoxyethyl)amino]-2-methyl-4oxobut-2-enoyl.
 6. The compoundof claim 2, wherein R4 and R10 are independently CH2O-angeloyl,CH2O-tigloyl, CH2O-senecioyl, CH2O-crotonoyl, CH2O-3,3-Dimethylacryloyl,CH2O-cinnamoyl, CH2O-pentenoyl, CH2O-hexanoyl, CH2O-benzoyl, orCH2O-ethylbutyryl O-4-(dimethylamino)-2-methylbut-2-enoyl,O-4-[(2-methoxyethyl)amino]-2-methyl-4oxobut-2-enoyl.
 7. The compound ofclaim 2, wherein the compound is selected from the following: a) Acompound having structure:

b) A compound having structure:

c) A compound having structure:

d) A compound having structure:

e) A compound having structure:

f) A compound having structure:


8. The compound of claim 2, wherein the compound is selected from thefollowing: a) A compound having structure:

b) A compound having structure:

c) A compound having structure:

d) A compound having structure:

e) A compound having structure:

f) A compound having structure:


9. The compound of claim 2, wherein the compound is selected from thefollowing: a) A compound having structure:

b) A compound having structure:

c) A compound having structure:

d) A compound having structure:

e) A compound having structure:

f) A compound having structure:


10. The compound of claim 2, wherein the compound is selected from thefollowing: a) A compound having structure:

b) A compound having structure:

c) A compound having structure:

d) A compound having structure:

e) A compound having structure:

f) A compound having structure:

g) A compound having structure:


11. The compound of claim 2, wherein the compound is attached one ormore of the functional groups selected from the group consisting ofangeloyl, tigloyl, senecioyl, acetyl, crotonoyl, Dimethylacryloyl,cinnamoyl, pentenoyl, hexanoyl, benzoyl, ethylbutyryl, alkyl, phenyl4-(dimethylamino)-2-methylbut-2-enoyl,4-[(2-methoxyethyl)amino]-2-methyl-4oxobut-2-enoyl; wherein the compoundcan be obtained by a method comprising the following steps: 1.Dissolving core compound, triterpenes, hydroxylated triterpenes core inpyridine;
 2. Adding the functional group or acyl chloride;
 3. Stirringthe mixture for a length of time including 5 sec, 1 min, 2 min, 5 min,10 min, 30 min, 1 hr, 2 hr, 18 hr, 2 days or 3 days at differenttemperature;
 4. At the end of reaction, adding an aqueous solution ofacid or base, or water to the reaction mixture;
 5. Extracting thesolution with ethyl acetate followed by lyophilization;
 6. Dissolvingthe reaction product in acetonitrile with Trifluoroacetic acid or DMSO;7. Testing the reaction product of mixtures and individual fractionswith MTT cytotoxic assay;
 8. Selecting the HPLC fractions for isolationaccording to the cytotoxic activity of the reaction product obtained ata specific reaction time;
 9. Purifying the active esterificationproducts with HPLC;
 10. Collecting the products; and
 11. Testing theproducts.
 12. The compound of claim 2, for treating cancer, inhibitingcancer growth, inhibiting cancer invasion, inhibiting cancer metastasis,modulating cell adhesion, modulating cell attachment, wherein the canceris selected from the group of breast cancer, leukocytic cancer, livercancer, ovarian cancer, bladder cancer, prostatic cancer, skin cancer,bone cancer, brain cancer, leukemia cancer, lung cancer, colon cancer,CNS cancer, melanoma cancer, renal cancer, cervical cancer, esophagealcancer, testicular cancer, spleenic cancer, kidney cancer, lymphhaticcancer, pancreatic cancer, stomach cancer and thyroid cancer; whereinthe cells is selected from the group of breast cell, leukocytic cell,liver cell, ovarian cell, bladder cell, prostatic cell, skin cell, bonecell, brain cell, leukemia cell, lung cell, colon cell, CNS cell,melanoma cell, renal cell, cervical cell, esophageal cell, testicularcell, spleenic cell, kidney cell, lymphhatic cell, pancreatic cell,stomach cell and thyroid cell.
 13. The compound of any of claim 1,wherein the compound for treating cancers, inhibition of cancer growth,cancer invasion, cells invasion, cancer cell invasion; cell adhesion,cell attachment, cell circulating; for inhibiting viruses; forpreventing cerebral aging; for improving memory; improving cerebralfunctions; for curing enuresis, frequent micturition, urinaryincontinence; dementia, Alzheimer's disease, autism, brain trauma,Parkinson's disease or other neurodegenerative diseases caused bycerebral dysfunctions; for treating arthritis, rheumatism, poorcirculation, arteriosclerosis, Raynaud's syndrome, angina pectoris,cardiac disorder, coronary heart disease, headache, dizziness, kidneydisorder; cerebrovascular disease; inhibiting NF-kappa B activation; fortreating brain edema, severe acute respiratory syndrome, respiratoryviral diseases, chronic venous insufficiency, hypertension, chronicvenous disease, oedema, inflammation, hemorrhoids, peripheral edemaformation, varicose vein disease, flu, post traumatic edema andpostoperative swelling; for inhibiting blood clots, for inhibitingethanol absorption; for lowering blood sugar; for regulatingadrenocorticotropin and corticosterone levels; for Anti-MS,anti-aneurysm, anti-asthmatic, anti-oedematous, anti-inflammatory,anti-bradykinic, anti-capillarihemorrhagic, anti-cephalagic,anti-cervicobrachialgic, anti-eclamptic, anti-edemic, anti-encaphalitic,anti-epiglottitic, anti-exudative, anti-flu, anti-fracture,anti-gingivitis, anti-hematomic, anti-herpetic, anti-histaminic,anti-hydrathritic, anti-meningitic, antioxidant, anti-periodontic,anti-phlebitic, anti-pleuritic, anti-raucedo, anti-rhinitic,anti-tonsilitic, anti-ulcer, anti-varicose, anti-vertiginous,cancerostatic, corticosterogenic, diuretic, fungicide, hemolytic,hyaluronidase inhibitor, lymphagogue, natriuretic, pesticide, pituitarystimulant, thymolytic, vasoprotective, inhibiting leishmaniases,modulating adhesion or angiogenesis of cells, anti-parasitic; increasethe expression of the genes: ANGPT2, DDIT3, LIF and NFKB1Z, andmanufacturing an adjuvant composition and venotonic treatment.
 14. Thecompound is selected from claim 2 as a medicament.
 15. A compositioncomprising an effective amount of compound of any of claim 2 as amedicament.
 16. The composition comprises compound of any of claim 2,further comprising a pharmaceutically acceptable carrier or diluent. 17.The compound is selected from claim 7 as a medicament.
 18. The compoundis selected from claim 8 as a medicament.
 19. The compound is selectedfrom claim 9 as a medicament.
 20. The compound is selected from claim 10as a medicament.